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122 protocols using 8 channel head coil

1

Structural Brain Imaging of Patients

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For patients, we acquired structural brain images. Twenty-nine patients received research-quality MRI or CT scans. For 19 of 29 patients, we collected high-resolution, whole-brain T1 -weighted MR images on a 3T Siemens Trio scanner (repetition time = 1620 ms, echo time = 3.87 ms, field of view = 192 × 256 mm, 1 × 1 × 1 mm voxels) using a Siemens 8-channel head coil. Three of 29 patients were contraindicated for a 3T environment, so we collected whole-brain T1 -weighted MR images on a 1.5T Siemens Sonata scanner (repetition time = 3000 ms, echo time = 3.54 ms, field of view = 24 cm, 1.25 × 1.25 × 1.2 mm voxels) using a Siemens 8-channel head coil. Seven of 29 patients were contraindicated for MRI and underwent whole-brain CT scans without contrast (60 axial slices, 3-5 mm slice thickness) on a 64-slice Siemens SOMATOM Sensation scanner. Two patients declined to receive research-quality scans, so we acquired recent clinical MRI (n = 1) and CT (n = 1) scans.
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2

Multimodal MRI Neuroimaging Protocol

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All the MRI data were collected on 3.0 Tesla MR scanners (Siemens, Erlangen, Germany) with 8-channel head coils at the Department of Radiology of the First Affiliated Hospital of Nanchang University. All participants were asked to keep still, and keep their eyes closed but not to fall asleep or think about anything during the MRI scan. Foam pads were used to reduce head movements, and earplugs were used to decrease the noise. First, conventional axial T2-weighted imaging [repetition time (TR) = 4000 ms, echo time (TE) = 113 ms, thickness = 5 mm, gap = 1.5 mm, FOV = 220 mm × 220 mm, slices = 19] and axial T1-weighted imaging [TR = 250 ms, TE = 2.46 ms, thickness = 5 mm, gap = 1.5 mm, field-of-view (FOV) = 220 mm × 220 mm, slices = 19] were performed. Then, high-resolution three-dimensional T1-weighted images were obtained using a brain volume sequence (TR = 1900 ms, TE = 2.26 ms, thickness = 1.0 mm, gap = 0.5 mm, FOV = 250 mm × 250 mm, matrix = 256 × 256, flip angle = 9°, 176 sagittal slices). Finally, resting-state fMRI data were collected using an echo-planar imaging sequence with the following parameters: TR = 2000 ms, TE = 30 ms, flip angle = 90°, FOV = 230 mm × 230 mm, matrix = 64, thickness = 4 mm, gap = 1.2 mm. Each brain volume consisted of 30 axial sections, and each functional run comprised 240 volumes.
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3

Multimodal MRI Protocol for Brain Imaging

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All the MRI datas were collected on 3.0 Tesla MR scanners (Siemens, Erlangen, Germany) with 8-channel head coils. All participants laid down on the scanning bed; earplugs were used to minimize scanner noise, and foam pads were used to reduce head movements. The participants were asked to stop thinking as soon as possible and to closed their eyes, but remain awake during MRI scan. First, conventional T2-weighted images (repetition time (TR) = 4000 ms, echo time (TE) = 113 ms, slices = 19, 5-mm slice thickness, 1.5-mm gap, field of view (FOV) = 220 mm × 220 mm) and T1-weighted images (TR = 250 ms, TE = 2.46, the same slices, slice thickness, gap, and FOV to T2WI) and were acquired. A total of 176 high-resolution T1-weighted images were collected using sagittal orientation three-dimensional spoiled gradient-recalled echo sequence (TR = 1900 ms; TE = 2.26 ms; 1.0-mm thickness with no gap; acquisition matrix = 256 × 256; FOV = 250 mm × 250 mm, flip angle = 9°); 30 axial slices and 240 functional images were obtained using gradient-recalled echo-planar imaging pulse sequence (TR = 2000 ms; TE = 30 ms; 4.0-mm thickness; 1.2-mm gap; acquisition matrix = 64 × 64; FOV =230 mm × 230 mm; flip angle =90°).
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4

Resting-State fMRI Brain Imaging Protocol

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A superconducting MR scanner (produced by Siemens, Germany), with an 8-channel head coil, was used for brain data collection. The research objects were instructed to stay awake and relax during the image acquisition process. The patients from both groups underwent routine T1-weighted imaging (T1WI) and T2-weighted imaging (T2WI). Besides, the gradient field strength was 40 mT/m, gradient switching rate was 150 mT/m/ms, axial view was T2WI image, repetition time = 4,000 ms, echo time = 113 ms, slice thickness = 5.0 mm, field of view = 225 mm × 225 mm, and matrix = 320 × 300. T2 gradient echo sequence was applied in the brain resting-state fMRI scanning, including repetition time = 2,000 ms, echo time = 30 ms, reversal angle = 90°, field of view = 220 mm × 220 mm, matrix was 64 × 64, and there was interval scanning of 30-slice cross section, with the layer thickness of 4.0 mm and layer spacing of 1.2 mm.
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5

fMRI Acquisition and Preprocessing Protocol

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Images were acquired at the “CERMEP-Imagerie du vivant” imaging center of Lyon (France) on a 1.5T Siemens Sonata scanner with a standard 8-channel head coil. For fMRI, echo planar T2*-weighted axial images were acquired with the following parameters: 26 interleaved slices; repetition time = 2500 ms; echo time = 60 ms; field of view = 240 mm2; flip angle = 90°; matrix = 64 × 64 (voxel size: 3.75 × 3.75 × 4 mm). Manual shimming was performed on the whole brain to improve the local field homogeneity and minimize susceptibility artifacts. Before the first fMRI session, a 3D T1-weighted anatomical scan was acquired (176 axial slices; repetition time = 1970 ms; echo time = 3.93 ms; field of view = 256 mm2; voxel size = 1 mm3).
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6

Multimodal Neuroimaging Acquisition Protocol

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Neuroimaging data were prospectively acquired with a Siemens 3 T Verio scanner using an 8-channel head coil. For gray and white matter analyses we acquired two T1-weighted scans (176 contiguous slices, 1 mm3 voxels, TR/TE = 2500/3.77 ms). For diffusion analyses we acquired two separate single-shot EPI diffusion weighted images with gradients applied along six directions (b = 100 s/mm2) and 64 directions (b = 1000 s/mm2) respectively. Diffusion imaging parameters were set at 73 contiguous axial slices with 2 mm3 voxels and TR/TE = 17,300/81 ms. For skull segmentation, we acquired T2-weighted images with the following parameters: 176 contiguous slices, 1 mm3 voxels, TR/TE = 3200/409 ms.
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7

Structural MRI Acquisition Protocol

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All the participants were scanned on a 3-T MRI system (Siemens Trio, Munich, Germany) with the same sequence and an 8-channel head coil. A high-resolution magnetization-prepared rapid gradient echo (MPRAGE) T1-weighted sequence was used for morphological MRI data acquisition with the following parameters: repetition time = 1900 ms; echo time = 2.26 ms; flip angle = 9°; field of view = 240 × 240 mm; matrix size = 256 × 256; thickness = 1.0 mm; and 176 sagittal slices without interslice gap.
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8

Structural and Functional Neuroimaging with 3T MRI

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We used a 3T MRI scanner (Trio, Siemens Medical Solutions, Erlangen, Germany) with an 8-channel head coil for all measurements. Structural images were acquired using a T1-weighted 3D magnetization-prepared-rapid-gradient-echo sequence. The parameters used were: flip angle = 8°, voxel size = 1 mm isotropic, TR = 2000 ms, TI = 990 ms, TE = 4.38 ms, and number of voxels = 208 × 256 × 208. Functional images were acquired with a T2-weighted echo-planar imaging sequence. The parameters used were: flip angle = 90°, voxel size = 3 mm (isotropic, with no slice gap), TR = 3000 ms, TE = 30 ms, and number of voxels = 64 × 64 × 44. The slices were acquired in interleaved order.
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9

3T Functional and Structural MRI Protocol

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MR images were acquired with a 3T Siemens Trio MRI scanner with an 8-channel head coil (Siemens Medical, Erlangen, Germany). T2-weighted gradient echo planar imaging (EPI) were used to obtain the functional images (TR/TE = 2000 ms/30 ms; Flip angle = 90°; Slices = 32; Field of view = 220 mm × 220 mm; matrix size = 64 × 64; Voxel size = 3.4 mm × 3.4 mm × 4 mm). High resolution T1-weighted anatomical images were also recorded for anatomical reference (TR/TE = 1900 ms/2.52 ms; Flip angle = 9°; Slices Thickness = 1 mm; Slices = 176; Resolution matrix = 256 mm × 256 mm; Voxel size = 1 mm × 1 mm × 1 mm).
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10

Multimodal Neuroimaging of MS Patients

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Patients and HC underwent 11C-PBR28 MR-PET imaging on a Siemens (Erlangen, Germany) BrainPET system, a PET scanner operating in the bore of a 3T whole-body MRI system equipped with an 8-channel head coil19 (link). All subjects received an intravenous bolus injection of 11C-PBR28, which was produced in house (mean±SD administered dose= 11.4±0.6mCi in patients and 11.06±0.8 mCi in HC, p= 0.1), as previously detailed15 (link). The PET data were acquired in list-mode format during a 90 minutes scan. Multiple gradient echo 3-dimensional (3D) magnetization-prepared rapid acquisition (ME-MPRAGE) images (1 mm isotropic voxels) for attenuation correction20 , co-registration of PET maps to 3T data, FreeSurfer anatomical reconstruction for brain cortex and WM segmentation, and conventional 3D fluid-attenuated inversion recovery (FLAIR) images (1 mm isotropic voxels) for brain WM lesions segmentation, were simultaneously acquired to PET images. Patients also underwent 7T MRI on a Siemens scanner using a 32-channel head coil, within ~1 week from the MR-PET session to acquire 0.6×0.6×1.5 mm voxels T1-weighted Turbo-FLASH (TFL) images for cerebellar lesion segmentation. Three MS patients did not undergo 7T imaging due to the presence of implants not approved for 7T.
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