Arachidonyl trifluoromethyl ketone (AACOCF3), acetyl salicylic acid, diethylumbelliferyl phosphate (DEUP), ginkgolide B, ibuprofen, platelet-activating factor (PAF), 1-O-Hexadecyl-2-acetyl-sn-glycerol-3-phospho-(N,N,N-trimethyl)-hexanolamine (Hexa-PAF), methyl arachidonyl fluorophosphonate (MAFP), acetyl salicylic acid, ibuprofen, simvastatin, and squalene were all obtained from Sigma-Aldrich. Squalestatin was a gift from GlaxoSmithKline (Uxbridge, Middlesex, UK). Stock solutions were dissolved in ethanol or di-methyl sulphoxide and diluted in medium to obtain final working concentrations. Vehicle controls consisted of equivalent dilutions of ethanol or di-methyl sulphoxide in culture medium.
Ibuprofen
Manufactured by Merck Group
Sourced in United States, Germany, United Kingdom, Italy, India, Switzerland, Australia, China, France, Spain, Macao, Portugal, Poland
Ibuprofen is a laboratory-grade chemical compound used as a reference standard in analytical testing. It is a white, crystalline solid with a melting point of around 78°C. Ibuprofen is a common non-steroidal anti-inflammatory drug (NSAID) and is often used as a calibration standard for the identification and quantification of pharmaceutical and biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Ketoprofen, by Merck Group (29 mentions)
Diclofenac, by Merck Group (28 mentions)
Naproxen, by Merck Group (24 mentions)
Warfarin, by Merck Group (24 mentions)
Indomethacin, by Merck Group (19 mentions)
Caffeine, by Merck Group (19 mentions)
Methanol, by Merck Group (18 mentions)
Carbamazepine, by Merck Group (18 mentions)
Sodium hydroxide (naoh), by Merck Group (18 mentions)
Paracetamol, by Merck Group (17 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (16 mentions)
Bovine serum albumin (bsa), by Merck Group (16 mentions)
Formic acid, by Thermo Fisher Scientific (12 mentions)
Acetylsalicylic acid, by Merck Group (12 mentions)
Acetaminophen, by Merck Group (12 mentions)
Human serum albumin (hsa), by Merck Group (11 mentions)
Formic acid, by Merck Group (11 mentions)
Acetonitrile, by Merck Group (11 mentions)
Diclofenac sodium salt, by Merck Group (11 mentions)
Sodium chloride (nacl), by Merck Group (10 mentions)
272 protocols using ibuprofen
1
Lipidomic Analysis of Cell Cultures
2
Prostate, Breast, and Hepatic Cancer Cell Cultures
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Ibuprofen, cabazitaxel, vincristine, tramadol, Ibuprofen, fluoxetine, aspirin and carboxymethyl cellulose (CMC) were purchased from Sigma Aldrich (Taufkirchen, Germany). Solvents i.e., dimethylsulfoxide (DMSO), n-hexane, ethyl acetate and ethanol were purchased from Sigma Aldrich. Unless stated otherwise, almost all solvents and chemicals were purchased from Sigma Aldrich. ISP4 medium was prepared in the laboratory. Distilled and/or deionized water was freshly prepared.
Prostate Cancer ((DU145 (HTB-81) and PC3 (CRL-1435)), breast cancer ((MCF-7 (HTB-22)) and hepatic cancer ((HuH-7 (CCL-185) and HuH-7.5 (PTA-8561) were used in the study. Cancer cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM), Streptomycin sulfate 100 μg/mL, amphotericin Bin Dulbecco’s Modified Eagle Medium (DMEM; 100 μg/mL streptomycin sulfate, 100 IU/mL penicillin G sodium, 0.25 μg/mL amphotericin B 0.25 μg/mL. penicillin G Sodium 100 IU/mL). RPMI-1640 medium (2.2 g/L sodium bicarbonate, pH 7.4) supplemented with 10% v/v HIFBS (heat inactivated fetal bovine serum) in a humidified incubator (37 °C, 5% CO2). Lymphocytes and macrophages were freshly isolated.
Prostate Cancer ((DU145 (HTB-81) and PC3 (CRL-1435)), breast cancer ((MCF-7 (HTB-22)) and hepatic cancer ((HuH-7 (CCL-185) and HuH-7.5 (PTA-8561) were used in the study. Cancer cells were grown and maintained in Dulbecco’s Modified Eagle Medium (DMEM), Streptomycin sulfate 100 μg/mL, amphotericin Bin Dulbecco’s Modified Eagle Medium (DMEM; 100 μg/mL streptomycin sulfate, 100 IU/mL penicillin G sodium, 0.25 μg/mL amphotericin B 0.25 μg/mL. penicillin G Sodium 100 IU/mL). RPMI-1640 medium (2.2 g/L sodium bicarbonate, pH 7.4) supplemented with 10% v/v HIFBS (heat inactivated fetal bovine serum) in a humidified incubator (37 °C, 5% CO2). Lymphocytes and macrophages were freshly isolated.
3
Solubilization of Pharmaceutical Compounds
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Model chemicals were reagent-grade caffeine, American Chemical Society reagentgrade benzoic acid (both Sigma-Aldrich), and ≥98% ibuprofen (Sigma). Amphibian Ringer's solution (ARS) was prepared according to published methods (Wright and Whitaker 2001; 6.6 g/L sodium chloride, 0.15 g/L potassium chloride, 0.15 g/L calcium chloride, 0.2 g/L sodium bicarbonate), spiked with 2.75 g/L 2-hydroxypropyl-beta-cyclodextrin (HPβCD;
Aldrich Chemistry) to assist solubilization in experiments using ibuprofen. Ethyl 3aminobenzoate methanesulfonate solution (MS-222, 0.02 g/L; Aldrich Chemistry) was buffered to pH 7.3 with sodium bicarbonate. Methanol and acetonitrile were highperformance liquid chromatography (HPLC) grade (Fisher Chemicals and Thermo Fisher Scientific), formic acid was analytical grade (Thermo Fisher Scientific), and the water used in HPLC analyses was ultrapure (Milli-Q Integral; Millipore). All solutions were freshly prepared.
Aldrich Chemistry) to assist solubilization in experiments using ibuprofen. Ethyl 3aminobenzoate methanesulfonate solution (MS-222, 0.02 g/L; Aldrich Chemistry) was buffered to pH 7.3 with sodium bicarbonate. Methanol and acetonitrile were highperformance liquid chromatography (HPLC) grade (Fisher Chemicals and Thermo Fisher Scientific), formic acid was analytical grade (Thermo Fisher Scientific), and the water used in HPLC analyses was ultrapure (Milli-Q Integral; Millipore). All solutions were freshly prepared.
4
Characterization of Drug Interactions
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All reagents, including ibuprofen, digitoxin, phenylbutazone, Ludox®, TFV, TDF, TAF, and HSA (purity ≥ 99%, catalog number A3782), were provided by Merck KGaA company (Darmstadt, Germany) and used without further purification. The phosphate buffer solution (PBS) was prepared with 137 mM sodium chloride (NaCl), 2.7 mM potassium chloride (KCl), 8 mM disodium phosphate (Na2HPO4), and 2 mM potassium dihydrogen phosphate (KH2PO4) to achieve pH 7.4.
5
Quantitative Analysis of Paracetamol and Ibuprofen
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Standards of paracetamol and ibuprofen were obtained from Merck, Germany with >99% purity. A stock solution was prepared with 1mg of paracetamol and 10mg of ibuprofen into a beaker and dissolved with 5ml of the mobile phase, acetonitrile (1% acetic acid in water (65:35 v/v)). The solution was then transferred quantitatively into a 100ml flask and topped up to the mark. Using serial dilution, the following calibration solution concentrations were prepared, paracetamol (ranging from 0 - 10 ug/ml) and ibuprofen (ranging from 0 - 16 ug/ml).
6
Comprehensive Chemical Reagents Procurement
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Tetracycline, erythromycin, benzoyl peroxide, crystal violet, actinomycin D, dimethyl sulfoxide (DMSO), Tris, KCl, 5,5’-dithio-bis-(2-nitrobenzoic acid) (DTNB), DMPTB, Triton X-100, ibuprofen, porcine hematin, L-epinephrine, formic acid, Na2EDTA, Griess reagent, citric acid, Na2HPO4, NaCl, BSA, NaOH, and CTAB were purchased from Merck SA (Pty) Ltd. (Sandton, Johannesburg, SA). PrestoBlue was purchased from Thermo Fisher Scientific (Randburg, South Africa). TRIzol and molecular grade water were purchased from Thermo Fisher Scientific (Waltham, Massachusetts, USA). Dexamethasone was purchased from Sigma Chemicals Co. (St. Louis, Missouri, USA). Ultra pure liquid chromatography water and acetonitrile (Romil-UpS™) were purchased from Microsep, South Africa. formic acid (99% purity) was purchased from Thermo Scientific, South Africa. Fluka® Analytical Ammonium hydroxide ≥ 25% in H2O, eluent additive for liquid chromatography (LC) was purchased from Sigma-Aldrich, South Africa.
7
Zeolite X and Y for Drug Adsorption
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Zeolite X and Y were supplied by SPAG, Iran. Ibuprofen and indomethacin was purchased from Merck, Germany. All chemicals and solvents were obtained from commercially available suppliers (Merck, Germany) and used without further purification.
8
Analytical Method Development for Pharmaceuticals
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Ibuprofen (IBF), fenoprofen calcium (FPC), methocarbamol (MTC), clonazepam (CZP), ferric chloride hexahydrate, ferrous chloride dehydrate, sodium acetate, aqueous ammonia solution (NH4OH), acetonitrile, methanol, ethanol, acetone, cetyltrimethylammonium bromide (CTAB), tetraethylorthosilicate (TEOS), hydrochloric acid, and 3, 5-dinitrosalicylic acid (DNS) were purchased from Merck (Darmstadt, Germany).
Aminopropyltrimethoxysilane (APTMS), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and pluronic P123 (EO20–PO70–EO20) were obtained from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of target analytes (100 mg L−1) was prepared in ethanol and working standard solutions were prepared by dilution of stock solution with double distilled water (DDW).
Aminopropyltrimethoxysilane (APTMS), 2, 2-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), and pluronic P123 (EO20–PO70–EO20) were obtained from Sigma-Aldrich (St. Louis, MO, USA). A stock solution of target analytes (100 mg L−1) was prepared in ethanol and working standard solutions were prepared by dilution of stock solution with double distilled water (DDW).
9
Synthesis and Characterization of Functionalized Polymers
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Pluronic F127 (MW = 12,600, 70% PEG, composition confirmed with proton (1H) nuclear magnetic resonance (NMR) spectroscopy), allyl bromide, sodium hydride, 2,2′-Azobis(2-methylpropionitrile) (AIBN), thioacetic acid (purum, ≥ 95.0% (GC)), Tetronic 701 (MW = 3600, 10% PEG), acryloyl chloride (purity: 97.0%, contains <210 ppm MEHQ as stabilizer), triethylamine (purity ≥ 99.5%), tris(2-carboxyethyl)phosphine hydrochloride (TCEP) (purity 98%), sodium hydroxide (purity ≥ 97%, pellets), hydrochloric acid (37%), potassium phosphate monobasic, sodium phosphate dibasic, toluene, N,N-Dimethylformamide, ammonium persulfate (APS), tetramethylethyldiamine (TEMED), and ibuprofen (purity 98%) were purchased from Merck (Sigma-Aldrich, Milan, IT, USA) and used as received.
All the functionalized polymers obtained were characterized using a 400 MHz Bruker 1H NMR spectrometer and a Thermo Scientific Nicolet iS50 Fourier-transform infrared (FT-IR) spectrometer in Attenuated total reflection (ATR) mode. Dynamic light scattering (DLS) measurements were carried out with Malvern Zetasizer ZS (Malvern, UK). Size Exclusion Chromatography (SEC) analysis was performed with the Jasco GPC System in tetrahydrofuran (THF) at a flow rate of 1 mL/min at 35 °C, calibrated using polystyrene standards.
All the functionalized polymers obtained were characterized using a 400 MHz Bruker 1H NMR spectrometer and a Thermo Scientific Nicolet iS50 Fourier-transform infrared (FT-IR) spectrometer in Attenuated total reflection (ATR) mode. Dynamic light scattering (DLS) measurements were carried out with Malvern Zetasizer ZS (Malvern, UK). Size Exclusion Chromatography (SEC) analysis was performed with the Jasco GPC System in tetrahydrofuran (THF) at a flow rate of 1 mL/min at 35 °C, calibrated using polystyrene standards.
10
Pharmaceutical Reference Material Preparation
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Acetylsalicylic acid for the standard preparation was purchased from TCI (Zwijndrecht, Belgium) with a purity ˃ 98.0%. Ibuprofen and hydrocortisone as the pharmaceutical reference materials were obtained from Merck. Ethanol absolute was purchased from Riedel-de Haën (Seelze, Germany). MiliQ® water (18.2 MΩcm−1; purified by MiliQ water purification system (Millipore, Bedford, MA, USA)) and HPLC gradient-grade methanol (J.T.Baker, Center Valley, PA, USA) were used with analytical-grade formic acid (FA) (Acros Organics, Geel, Belgium) for the mobile phase preparation. Stock solutions of acetylsalicylic acid, Ibuprofen, and hydrocortisone were prepared as 1 mg/mL solutions in methanol.
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