The soluble sugars were analyzed by high performance liquid chromatography (HPLC) coupled to a pulse amperometric detector according to Shiga et al. [38 (link)]. The organic acid contents were analyzed by HPLC in a HP1100 system (Hewlett-Packard Company, Palo Alto, CA, USA) coupled with a diode-array detector, equipped with a μBondpack C18 (300 mm × 3.6 mm i.d., Waters, Milford, MA, USA) and elution (flow rate of 0.5 mL.min−1) was carried out in isocratic conditions with 0.1% H3PO4, monitored at 210 nm. The content of total dietary fiber (TDF) and fractions were measured according to the method described by Association of Official Analytical Chemists (AOAC) (AOAC 991.43) [39 ].
Hp1100 system
Manufactured by Hewlett-Packard
Sourced in United States
The HP1100 system is a versatile laboratory equipment designed for a range of analytical applications. It provides reliable and precise data processing capabilities. The core function of the HP1100 system is to perform various analytical tasks, such as chromatography, spectroscopy, and other laboratory-based experiments.
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Lab products found in correlation
5 protocols using hp1100 system
1
Quantification of Soluble Sugars and Organic Acids
2
HPLC Analysis of 1-Deoxynojirimycin
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HPLC analysis was performed using an HP-1100 system (Hewlett Packard, Palo Alto, CA, USA). A reversed phase column (Eclipse XDB-C18, 150 mm × 4.6 mm id, 5 μm; Agilent, Palo Alto, CA, USA) was connected with a guard column (Eclipse XDB-C18, 4.0 mm × 4.6 mm id, 5 μm; Agilent, Palo Alto, CA, USA). An isocratic mobile phase system composed of acetonitrile and 0.1% v/v trifluoroacetic acid at a ratio of 45:55 was used to elute the sample at a flow rate of 1 mL/min. All samples were detected for UV absorbance (Hewlett Packard, Palo Alto, CA, USA) at 254 nm. 1-Deoxynojirimycin was used as a marker for quantitative analysis. All experiments were performed in triplicate.
3
HPLC Quantification of Ocimum sanctum
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HPLC analyses were performed using an HP1100 system with a UV detector set at 250 nm (Hewlett Packard, Palo Alto, CA, USA). A reversed phase column, (Eclipse XDB-C18 150 mm × 4.6 mm id, 5 μm Agilent, Palo Alto, CA, USA), was connected with an (Eclipse XDB-C18 guard column 4.0 mm × 4.6 mm id, 5 μm Agilent, Palo Alto, CA, USA) A gradient mobile phase system consisting of 0.5% formic acid (phase A) and acetonitrile (phase B) at a flow rate of 1.0 mL/min and 20 µL of 1 mg/mL O. sanctum ethanolic solution were injected. Each sample and mobile phase was filtrated through a 0.45-mm millipore filter, type GV (Millipore, Bedford, MA) prior to the HPLC injection. The gradient elution program was 90% A (0–2 min), 75% A (2–5 min) 0% A (5–8 min), and 90% A (8–10 min). All samples were analyzed in triplicate. RA was used as a marker for the quantitative analysis of O. sanctum ethanolic extract.
4
RP-HPLC Analysis of Compounds
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Chromatographic experiments were performed by RP-HPLC analysis, with Hewlett-Packard HP1100 system (Palo Alto, CA, USA) consisting of a quaternary pump, continuous vacuum degasser, equipped with a Rheodyne 7125 manual sample injector and a Hewlett-Packard HP UV–vis diode array detector (DAD). A HP ChemStation data system was used for data acquisition and handling. Chromatographic separations were achieved using a LiChroCART Purospher Star RP18-e column (250 mm × 4.6 mm i.d.) (5 µm) (Merck, Darmstad, Germany) combined with a Merck LiChroCART 4-4 LiChrospher 100 RP18 (5 µm) guard column. Elution was carried out isocratically with a mobile phase acetonitrile:water (90:10, v/v) containing 0.1% trifluoroacetic acid, at a flow rate 2 mL/min, and an injection volume of 20 µL. Detection and chromatographic acquisitions were performed at 406 nm, and peak spectra were recorded by setting the diode array detector from 190 to 500 nm. Identification was carried out by comparing retention times (Rt) and UV absorbance spectra with the reference standard.
5
Vitamin E Quantification by HPLC
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The content of vitamin E in the diets was quantified following AOCS method C-8-89 using High Performance Liquid Chromatography (HPLC) employing a Hewlett Packard HP 1100 system (Hewlett-Packard, Palo Alto, CA, USA) with fluorescence detector (excitation 259 nm, emission 325 nm) and a YMC 150 × 6.0 mm column [15 (link)].
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