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Ot 1 cd8 t cell receptor tg

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The OT-I CD8+ T cell receptor (TCR)-Tg is a laboratory tool used for research purposes. It consists of CD8+ T cells expressing a transgenic T cell receptor specific for the ovalbumin (OVA) peptide presented by the MHC class I molecule H-2Kb. This tool allows for the study of CD8+ T cell responses to the OVA antigen.

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Lab products found in correlation

Balb c mice, by Charles River Laboratories (3 mentions) Myd88, by Jackson ImmunoResearch (2 mentions) Cd11c cre tg, by Jackson ImmunoResearch (2 mentions) Tmem173gt, by Jackson ImmunoResearch (2 mentions) Cd11c itgax dtr, by Jackson ImmunoResearch (2 mentions) Balb c tg mmtv neu, by Jackson ImmunoResearch (2 mentions) Egfp tg mice, by Jackson ImmunoResearch (1 mentions) C57bl 6 female, by Charles River Laboratories (1 mentions)

3 protocols using ot 1 cd8 t cell receptor tg

1

Evaluating Tumor Growth in Mice

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Female C57BL/6 and BALB/c mice were purchased from Charles River Laboratories (Yokohama, Japan). OT-I CD8+ T cell receptor (TCR)-Tg, and EGFP-Tg mice were purchased from The Jackson Laboratory (Bar Harbor, ME, USA). The EGFP mice were crossed with OT-I mice to generate GFP-expressing OT-1 mice (GFP+ OT-1 mice). In all experiments, animals were randomly assigned to various experimental groups. For experiments designed to evaluate tumor growth, six to ten mice were used for each experiment, which were repeated twice. Animals were sacrificed at the end of the study. All mice were maintained under specific pathogen-free conditions and used when they were 6–12 weeks of age. All animal studies were performed in accordance with the procedures approved by the Animal Research Committee of the National Cancer Center (Tokyo, Japan).
Mashima H., Zhang R., Kobayashi T., Hagiya Y., Tsukamoto H., Liu T., Iwama T., Yamamoto M., Lin C., Nakatsuka R., Mishima Y., Watanabe N., Yamada T., Senju S., Kaneko S., Idiris A., Nakatsura T., Ohdan H, & Uemura Y. (2020). Generation of GM-CSF-producing antigen-presenting cells that induce a cytotoxic T cell-mediated antitumor response. Oncoimmunology, 9(1), 1814620.
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2

Murine Immune Cell Protocols

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Six- to eight-week old female C57BL/6J mice were purchased from Harlan or Jackson laboratory. Six- to eight-week old female Balb/c mice were purchased from Charles River laboratory in China. Myd88−/−, Trif−/−, Tmem173gt, OT-I CD8+ T cell receptor (TCR)-Tg, CD11c–Cre-Tg, CD11c(itgax)-DTR and Balb/c-Tg (MMTV-neu) mice were purchased from The Jackson Laboratory. ifnar1flox/floxmice were kindly provided by Dr. Ulrich Kalinke from the Institute for Experimental Infection Research, Hanover, Germany. All the mice were maintained under specific pathogen free conditions and used between 6–12 weeks of age in accordance to the animal experimental guidelines set by the Institutional Animal Care and Use Committee of the University of Chicago and Institute of Biophysics, CAS.
Liu X., Pu Y., Cron K., Deng L., Kline J., Frazier W.A., Xu H., Peng H., Fu Y.X, & Xu M.M. (2015). CD47 Blockade Triggers T cell-mediated Destruction of Immunogenic Tumors. Nature medicine, 21(10), 1209-1215.
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3

Murine Immune Cell Protocols

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Six- to eight-week old female C57BL/6J mice were purchased from Harlan or Jackson laboratory. Six- to eight-week old female Balb/c mice were purchased from Charles River laboratory in China. Myd88−/−, Trif−/−, Tmem173gt, OT-I CD8+ T cell receptor (TCR)-Tg, CD11c–Cre-Tg, CD11c(itgax)-DTR and Balb/c-Tg (MMTV-neu) mice were purchased from The Jackson Laboratory. ifnar1flox/floxmice were kindly provided by Dr. Ulrich Kalinke from the Institute for Experimental Infection Research, Hanover, Germany. All the mice were maintained under specific pathogen free conditions and used between 6–12 weeks of age in accordance to the animal experimental guidelines set by the Institutional Animal Care and Use Committee of the University of Chicago and Institute of Biophysics, CAS.
Liu X., Pu Y., Cron K., Deng L., Kline J., Frazier W.A., Xu H., Peng H., Fu Y.X, & Xu M.M. (2015). CD47 Blockade Triggers T cell-mediated Destruction of Immunogenic Tumors. Nature medicine, 21(10), 1209-1215.
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