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Tlr3 apc

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TLR3-APC is a flow cytometry antibody that targets the Toll-like receptor 3 (TLR3) protein. TLR3 is a pattern recognition receptor involved in the detection of double-stranded RNA, a molecular signature of viral infection. The APC conjugate allows for detection of TLR3-expressing cells using flow cytometry.

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Lab products found in correlation

Aurora spectral analyzer, by Cytek (2 mentions) True nuclear transcription buffer set, by BioLegend (2 mentions)

2 protocols using tlr3 apc

1

Maternal Immune Activation Impacts Fetal HSPCs

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8-12-week-old female C57BL/6 females were mated to C57BL/6 male mice and treated with 20mg/kg pIC or saline at E14.5. Females were sacrificed 2hrs later and FLs were harvested from embryos and processed as described above and. Cells were then stained using an HSPC antibody cocktail before being fixed and permeabilized with the True-Nuclear Transcription buffer set (Biolegend) and stained with TLR3-APC (Biolegend).
For TLR3 KO experiments, TLR3KO/KO female mice (RRID:IMSR_JAX:005,217) were mated to C57BL/6 males and treated with 20mg/kg pIC or saline at E14.5. Litters were aged to postnatal day (P)14, genotyped and sacrificed for BM HSPC analysis. Flow cytometric analysis was performed using an Aurora Spectral Analyzer (Cytek).
López D.A., Apostol A.C., Lebish E.J., Valencia C.H., Romero-Mulero M.C., Pavlovich P.V., Hernandez G.E., Forsberg E.C., Cabezas-Wallscheid N, & Beaudin A.E. (2022). Prenatal inflammation perturbs murine fetal hematopoietic development and causes persistent changes to postnatal immunity. Cell reports, 41(8), 111677.
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2

Maternal Immune Activation Impacts Fetal HSPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
8-12-week-old female C57BL/6 females were mated to C57BL/6 male mice and treated with 20mg/kg pIC or saline at E14.5. Females were sacrificed 2hrs later and FLs were harvested from embryos and processed as described above and. Cells were then stained using an HSPC antibody cocktail before being fixed and permeabilized with the True-Nuclear Transcription buffer set (Biolegend) and stained with TLR3-APC (Biolegend).
For TLR3 KO experiments, TLR3KO/KO female mice (RRID:IMSR_JAX:005,217) were mated to C57BL/6 males and treated with 20mg/kg pIC or saline at E14.5. Litters were aged to postnatal day (P)14, genotyped and sacrificed for BM HSPC analysis. Flow cytometric analysis was performed using an Aurora Spectral Analyzer (Cytek).
López D.A., Apostol A.C., Lebish E.J., Valencia C.H., Romero-Mulero M.C., Pavlovich P.V., Hernandez G.E., Forsberg E.C., Cabezas-Wallscheid N, & Beaudin A.E. (2022). Prenatal inflammation perturbs murine fetal hematopoietic development and causes persistent changes to postnatal immunity. Cell reports, 41(8), 111677.
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