Ecx 400 mhz instrument

All commercially available chemicals were used without further purification. Octamethylazacalix[8]pyridine (Py[8]) was synthesized according to the literature method by the [3 + 5] fragment coupling protocol between a terminal dibrominated linear trimer and a terminal diaminated linear pentamer.25 (link) The solvents used in this study were processed by standard procedures. ¹H and ¹³C NMR experiments were carried out on a JEOL ECX-400 MHz instrument. Elemental analyses were recorded on a Thermo FlashEA 1112 elemental analyzer. Mass spectra were obtained using a Thermo Scientific Exactive Orbitrap instrument. Infrared spectra were recorded on a Perkin-Elmer spectrum 100 FT-IR spectrometer.

All commercially available chemicals were used without further purification. The solvents used in this study were dried by standard procedures. All reactions were carried out under a nitrogen atmosphere unless otherwise noted. [O(AuPPh₃)₃](BF₄),18 2-(R,R)14 (link) and 3-(R,R)14 (link) were prepared according to the published methods. ¹H and ³¹P NMR experiments were carried out on a JEOL ECX-400 MHz instrument. The samples were heated to the target temperature for 10 min and ¹H and ³¹P NMR experiments were carried out in situ. CD and UV-vis measurements were carried out on a Jasco J-815 circular dichroism spectropolarimeter, using 1 cm cuvettes and with pre-dried dimethyl sulfoxide as solvent. The samples were heated to the target temperature for 10 min and the CD and UV-vis spectra were monitored in situ. For the CD measurements, two accumulations of standard sensitivity at a scan rate of 100 nm min^–1 and data pitch of 0.5 nm were averaged. Anisotropy factors were calculated according to g = θ [mdeg]/(32 980 × A) using the UV-vis spectrum data measured by the CD spectrometer. For time-course measurements, the change of temperature begins to detect the change of the CD response at specific wavelengths, which was followed in 0.5 s intervals at different temperatures. Mass spectra were obtained using a Thermo Scientific Exactive Orbitrap instrument.