Transient MCAo was performed as previously described with modifications80 (link). For stroke studies 2 weeks after siPtn or control siRNA ICV injections we used 30 minute MCAo and 24 hr reperfusion (Fig. 4l ,m ); for leucocyte and platelet control immunostaining we used 3 h MCAo and 24 h reperfusion (Fig. S5c ); for edema (ADC) values after stroke we used 1 h MCAo and 15 days reperfusion (Fig. S5g ). Infarction volume was determined as previously described80 (link). Fluoro-Jade B staining (EMD Millipore, AG310) was performed per manufacturer’s instructions.
Fluoro jade b staining
Manufactured by Merck Group
Fluoro-Jade B is a fluorescent stain used for the histological detection of degenerated neurons. It binds to degenerating neurons, allowing their visualization under a fluorescent microscope.
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2 protocols using fluoro jade b staining
1
Transient Middle Cerebral Artery Occlusion Model
2
Transient Middle Cerebral Artery Occlusion Model
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Transient MCAo was performed as previously described with modifications80 (link). For stroke studies 2 weeks after siPtn or control siRNA ICV injections we used 30 minute MCAo and 24 hr reperfusion (Fig. 4l ,m ); for leucocyte and platelet control immunostaining we used 3 h MCAo and 24 h reperfusion (Fig. S5c ); for edema (ADC) values after stroke we used 1 h MCAo and 15 days reperfusion (Fig. S5g ). Infarction volume was determined as previously described80 (link). Fluoro-Jade B staining (EMD Millipore, AG310) was performed per manufacturer’s instructions.
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