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Anti mouse elisa kit

Manufactured by R&D Systems
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The Anti-mouse ELISA kit is a laboratory tool used to detect and quantify mouse-specific proteins or analytes in biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique to provide a sensitive and accurate measurement of the target analyte.

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Lab products found in correlation

Ripa buffer, by Beyotime (1 mentions) Bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

ELISAs (144 citations) Mouse ELISA kits (102 citations) Anti-mouse TNF-α ELISA kits (2 citations) Anti-human or mouse quantitative ELISA kits (2 citations) Anti-mouse IL-1β Quantikine ELISA kit (2 citations)

6 protocols using anti mouse elisa kit

1

Quantifying Platelet Factor 4 Release

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The total amount of PF4 in platelet lysates was measured using the anti-mouse ELISA kit from R & D Systems (Minneapolis, MN) following the manufacturer’s instruction.
Xiang B., Zhang G., Ye S., Zhang R., Huang C., Liu J., Tao M., Ruan C., Smyth S.S., Whiteheart S.W, & Li Z. (2015). Characterization of a Novel Integrin Binding Protein, VPS33B, Which is Important for Platelet Activation and in vivo Thrombosis and Hemostasis. Circulation, 132(24), 2334-2344.
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2

Platelet PF4 Secretion Assay

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Washed platelets (3 × 108/ml) from C57BL/6J mice were pre-incubated with vehicle, EGTA, or RGDS at RT for 15 min and then stored at RT or 4°C for 24 h. After incubation, supernatant of washed platelets was collected by centrifuging at 1,000g for 4 min. The amount of PF4 in platelet supernatant was measured using the anti-mouse ELISA kit from R & D Systems (Minneapolis, MN) following the manufacturer’s instruction.
Xiang B., Zhang G., Zhang Y., Wu C., Joshi S., Morris A.J., Ware J., Smyth S.S., Whiteheart S.W, & Li Z. (2020). Calcium ion chelation preserves platelet function during cold storage. Arteriosclerosis, thrombosis, and vascular biology, 41(1), 234-249.
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3

Hepatic Vein Serum Cytokine Assay

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Blood was collected from hepatic veins and serum obtained by centrifugation for 15 minutes at 1500g at 4°C. Serum samples were stored at −80°C until analysis. Serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) were measured by Antech Diagnostics (Morrisville, NC). Cytokine levels were measured using an anti-mouse ELISA kit (RD Systems, Minneapolis).
Avni D., Harikumar K.B., Sanyal A.J, & Spiegel S. (2021). Deletion or inhibition of SphK1 mitigates fulminant hepatic failure by suppressing TNFα-dependent inflammation and apoptosis. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 35(3), e21415.
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4

Quantifying Lung Protein Levels

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The levels of TNFα and CXCL1 proteins in mouse lung tissue homogenates were quantified using anti-mouse ELISA kits ordered from R&D Systems. The measurements were conducted according to the manufacturer’s instructions. To ensure consistency, the obtained values were normalized to the total protein content as previously described [12 (link)]. To prepare human lung tissue homogenates, the lung tissues were rapidly frozen and homogenized on ice in RIPA buffer (Beyotime, #R0010) with phenylmethylsulfonyl fuoride (PMSF). Homogenates were sonicated (3 times in 5 s), centrifuged (500 g, 10 min at 4℃), and the central layer was collected. Total protein concentrations were determined using a BCA Protein Assay Kit (Thermo Fisher Scientific Pierce). RIPA buffer was used to dilute lung tissue homogenates to a final protein concentration of 500 mg/mL.
Lu F., Yao L.P., Gao D.D., Alinejad T., Jiang X.Q., Wu Q., Zhai Q.C., Liu M., Zhu S.M., Qian M.X., Xu L.F., Chen C.S, & Zhang F. (2024). MicroRNA-377-3p exacerbates chronic obstructive pulmonary disease through suppressing ZFP36L1 expression and inducing lung fibroblast senescence. Respiratory Research, 25, 67.
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5

Quantifying Lung IL-1β Levels in Mice

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Mouse lung IL‐1β protein levels were measured in lung tissue homogenates using anti‐mouse ELISA kits (R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.
Donovan C., Kim R.Y., Galvao I., Jarnicki A.G., Brown A.C., Jones‐Freeman B., Gomez H.M., Wadhwa R., Hortle E., Jayaraman R., Khan H., Pickles S., Sahu P., Chimankar V., Tu X., Ali M.K., Mayall J.R., Nguyen D.H., Budden K.F., Kumar V., Schroder K., Robertson A.A., Cooper M.A., Wark P.A., Oliver B.G., Horvat J.C, & Hansbro P.M. (2022). Aim2 suppresses cigarette smoke‐induced neutrophil recruitment, neutrophil caspase‐1 activation and anti‐Ly6G‐mediated neutrophil depletion. Immunology and Cell Biology, 100(4), 235-249.
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6

Cytokine Production in EAE Murine Model

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Spleens from the EAE WT and Nrf2 KO mice were harvested and mechanically disrupted to obtain single cell suspensions. Cells (2.5 × 106/ml) were stimulated with MOG35–55 (10 µg/ml) for 72 h in complete Roswell Park Memorial Institute (RPMI) 1640 (Gibco, Paisley, UK). Following culture, the cells were spun down, and supernatants were collected for enzyme-linked immunosorbent assay (ELISA). Following the manufacturer’s instructions, the concentrations of interleukin-6 (IL-6), IL-17, and interferon-gamma (IFN-γ) were determined using anti-mouse ELISA kits (R&D Systems, Minneapolis, MN).
Larabee C.M., Desai S., Agasing A., Georgescu C., Wren J.D., Axtell R.C, & Plafker S.M. (2016). Loss of Nrf2 exacerbates the visual deficits and optic neuritis elicited by experimental autoimmune encephalomyelitis. Molecular Vision, 22, 1503-1513.
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