TIAR (Santa Cruz, sc-1749), NPM (Santa Cruz, sc-70392), FBL (Cell signaling Technology, 2639), RPA194 (Santa Cruz, sc-4669), eIF2α (Santa Cruz, sc-133132), phospho-eIF2α (AbCam, 131505), 4EBP (Cell Signaling Technology, 9454), non-phospho-4EBP (Cell Signaling Technology, 4923S), TIF-IA/RRN3 (Santa Cruz, 2c-390464), RPL7A (Cell Signaling Technology, 2415) Nol9 (Protein Tech Group, 16083-1-AP), UBF (Santa Cruz, sc-13125), eIF3B (Santa Cruz, sc-16377)
Rpa194
Manufactured by Santa Cruz Biotechnology
Sourced in United Kingdom
RPA194 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a core component used in various scientific research applications. The detailed function and specifications of this product are not available at this time.
Automatically generated - may contain errors
Lab products found in correlation
Fibrillarin, by Abcam (3 mentions)
Fibrillarin, by Merck Group (2 mentions)
Cenpa, by Thermo Fisher Scientific (2 mentions)
Nis elements ar 4, by Nikon (2 mentions)
Coilin, by Santa Cruz Biotechnology (2 mentions)
Eif3b, by Santa Cruz Biotechnology (1 mentions)
Phospho eif2α, by Abcam (1 mentions)
Eif2α, by Santa Cruz Biotechnology (1 mentions)
Orf45, by Santa Cruz Biotechnology (1 mentions)
Phosphate buffered saline (pbs), by Sartorius (1 mentions)
Hoechst dye, by Merck Group (1 mentions)
Nucleolin, by Abcam (1 mentions)
Alexa fluor 488, by Jackson ImmunoResearch (1 mentions)
Cyanine3 (cy3), by Jackson ImmunoResearch (1 mentions)
Alexa fluor 647, by Jackson ImmunoResearch (1 mentions)
Eclipse ti e inverted, by Nikon (1 mentions)
Lsm880 fluorescence microscope, by Zeiss (1 mentions)
Eclipse ti e, by Nikon (1 mentions)
Brca1, by Santa Cruz Biotechnology (1 mentions)
Paxillin, by Merck Group (1 mentions)
8 protocols using rpa194
1
Protein Expression Analysis in Cell Lines
2
Immunocytochemistry of Nucleolar Proteins in mES Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
E14 mES cells were grown on gelatin-coated glass coverslips with MEFs and cultured 12 h before fixed with 4% PFA for 10 min, and then permeabilized with 0.5% Triton X-100 in PBS for 20 min at room temperature (RT). The cell samples were blocked in blocking buffer (3% BSA, 2% donkey serum in PBS) for 10 min at RT and then stained with a primary antibody (1:100, Nucleolin, CST, cat. no. 145745; 1:500, NPM1, Sigma, cat. no. B0556; 1:100, Fibrillarin, Abcam, cat. no. ab4566; RPA194, 1:200, Santa Cruz, cat. no. sc-48385) for 12 h at 4 °C. After three washes with 0.1% Triton X-100 in PBS, cells were stained with a secondary antibody (1:200, Goat polyclonal Secondary Antibody to Mouse IgG, Abcam, cat. no. ab150113) for 2–12 h at 4 °C. Followed by washing three times with 0.5% Triton X-100/PBS, DAPI was used for nucleus staining. The samples were then imaged by Zeiss LSM880 fluorescence microscope at a 63× oil objective. For high regulation microscopy imaging, LSM800 with Airyscan module was used.
3
Immunofluorescence Staining of Nuclear Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded on coverslips in a 24-well plate, washed with PBS (Biological Industries, Israel), and fixed with 4% formaldehyde in PBS for 25 min at room temperature. After fixation, cells were washed with PBS, and permeabilized and blocked in PBS containing 0.2% Triton X-100% and 1% BSA. Slides were incubated with primary antibodies to UBF (Sigma/Santa Cruz, CA, USA), Fibrillarin (Abcam, Cambridge, UK), NPM (Abcam, Cambridge, UK), Nucleolin (Abcam, Cambridge, UK), RPA194 (Santa Cruz, CA, USA), PICT-1 (Santa Cruz, CA, USA), PML (Santa Cruz, CA, USA), ORF45 (Santa Cruz, CA, USA), ORF65 [64 (link)] (a kind gift from Shou Jiang Gao) or ORF59 (a kind gift from Prof. Bala Chandran, University of South Florida) [65 (link)] at 40 C, followed by incubation with secondary conjugated antibodies (Rhodamine, Cy3, Alexa Fluor 647 or Alexa Fluor 488, Jackson ImmunoResearch Laboratories, Inc., West Grove, PA) for 1 h at room temperature. To stain the nuclei, cells were incubated for 10-min with 0.05 µg/mL Hoechst dye (Sigma) in PBS. The slides were mounted with anti-fading medium (1% n-Propyl gallate, 90% Glycerol in PBS). Cells were examined and photographed under a confocal laser-scanning microscope (Leica SP8 Confocal Live Microscope).
4
Immunofluorescence Staining of Nuclear Proteins
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were fixed in 4% paraformaldehyde for 15 min and washed in phosphate-buffered saline (PBS) three times of 10-min each before permeabilized by 0.2% Triton-X 100/PBS for 10 min and additional PBS wash three times. Primary antibodies were then incubated for 1 h at room temperature. They are from the indicated sources and used at the stated dilutions: RPA194, Santa Cruz Biotechnology, catalogue#sc-48385, 1:100; PTB (S. Huang laboratory), 1:300; UBF, Santa Cruz Biotechnology, catalogue#: sc-13125, 1:50; fibrillarin, Sigma, catalogue#: ANA-N, 1:10; CENPA (Thermo Fisher #MA1-20832, 1:100; coilin, Santa Cruz Biotechnology, catalogue#: sc-32860, 1:300; Flash histone locus protein (Joseph Gall, Carnegie Institution for Science), 1:500; SMN, Santa Cruz Biotechnology, catalogue# sc-15320, 1:300, SC-35 1:200 (David Spector, Cold Spring Harbor Laboratory). After incubation with primary antibodies, the fluorescein-conjugated (Jackson ImmunoResearch) and Alexa Fluor-labeled (ThermoFisher Scientific ) secondary antibodies were incubated with cells at a dilution of 1:200 for 1 h at room temperature. Cell bearing coverslips were then mounted with Vector mounting media. Slides were visualized on a Nikon Eclipse Ti-E inverted fluorescence microscope using NIS-Elements AR 4.2 software (Nikon).
5
Immunofluorescence Staining of Embryos
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Embryos were first fixed with 1 and 2% paraformaldehyde (PFA) in 1× PBS for 3 min sequentially, followed by treatment with 4% PFA for 30 min at room temperature (RT). Embryos were washed three times with 1× PBS, permeabilized for 15 min in PBS/0.25% Triton X-100 and blocked-in blocking buffer (PBS/0.2% BSA/0.01% Tween-20) for 1 h at RT, followed by incubation overnight with primary antibodies (1:200, Nucleolin, CST, 145745; 1:400, NPM1, Sigma, B0556; 1:200, Fibrillarin, Abcam, ab4566; RPA194, 1:50, Santa Cruz, sc-48385) at 4° or for 1 h at 37 °C. Subsequently, embryos were washed four times for 10 min each and incubated with a secondary antibody (daylight 488-conjugated anti-rabbit, 1:100 or daylight 594-conjugated anti-mouse, 1:200) for 1 h at 37 °C and washed three times with PBS. Nuclei were stained with DAPI for 1 min. Embryos were observed under Zeiss LSM880 fluorescence microscope at 63× magnification with an oil immersion objective.
6
Immunofluorescence Staining and Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Primary antibodies were from the indicated sources and used at the stated dilutions: RPA194, Santa Cruz Biotechnology, cat #sc-48385, 1:100; PTB (S. Huang laboratory), 1:300; UBF, Santa Cruz Biotechnology, cat#: sc-13125, 1:50; fibrillarin, Sigma, cat #: ANA-N, 1:10; CENPA (Thermo Fisher #MA1-20832, 1:100; coilin, Santa Cruz Biotechnology, cat#: sc-32860, 1:300; Flash histone locus antibody protein (Gall lab), 1:500; SMN, Santa Cruz Biotechnology, cat.# sc-15320, 1:300. The fluorescein conjugated (Jackson ImmunoResearch) and Alexa Fluor-labeled (ThermoFisher) secondary antibodies were used at a dilution of 1:200. Slides were visualized on a Nikon Eclipse Ti-E inverted fluorescence microscope using NIS-Elements AR 4.2 software (Nikon).
7
Polyclonal Antibody Production for HERC2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Rabbit polyclonal antibody against the C-terminal region of HERC2, generated against recombinant HERC2 protein (residues 4389–4834), was established as per previously described methods27 (link). The commercially available antibodies used in the study were: rabbit polyclonal antibodies against BLM (Bethyl Laboratories, A300-110A), WRN (Bethyl Laboratories, A300-239A), BRCA1 (Santa Cruz Biotechnology, C20), mouse monoclonal antibodies against HERC2 (epitope 1781–1974, BD Biosciences, 17), NPM1 (Zymed Laboratories Inc), FBL (Abcam, 38F3), RPA194 (Santa Cruz Biotechnology, C1), RPA2 (Calbiochem, RPA34-20 NA19L), and α-tubulin (Neomarkers, DM1A, MS-581-P).
8
Comprehensive Immunofluorescence Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Following primary antibodies were used in this study: lamin B (Santa Cruz cat. no. sc-6217); filamin (Santa Cruz cat. no. sc-28284); alpha-actinin-4 (Abcam cat. no. ab96866); spectrin (Sigma Aldrich cat. no. S1390); paxillin (Millipore cat. no. 05-471); vinculin (Sigma Aldrich cat. no. V4505); mDia1 (BD Biosciences cat. no. P66520-050); SUN2 (Abcam cat. no. ab124916); emerin (Abcam cat. no. ab40688); Arp3 (Welch et al. 1997 (link)); cofilin (Abcam cat. no. ab11062); P-cofilin (Cell Signaling cat. no. 3313); Arp6 (Sigma Aldrich cat. no. R35554); Arp5 (Kitayama et al. 2009 (link)); Arp8 (Aoyama et al. 2008 (link)); Brg1 (Abcam cat. no. ab70558); hnRNP U (Santa Cruz, clone 3G6); H3K9Me2 (Millipore cat. no. 17-648); H3K4Me2 (Millipore cat. no. 07-030); CTD-phosphoS2 (Abcam cat. no. ab24758); RPA194 (Santa Cruz, cat. no. sc-28714); and BrdU (Sigma Aldrich, clone BU-33).
Secondary antibodies used in this study are donkey anti-rabbit IgG conjugated with Alexa Fluor 568 (A10042), goat anti-mouse IgG conjugated with Alexa Fluor 647 (A21236) and donkey anti-goat IgG cojugated with Alexa Fluor 647 (A21447) all purchased from Life Sciences.
Secondary antibodies used in this study are donkey anti-rabbit IgG conjugated with Alexa Fluor 568 (A10042), goat anti-mouse IgG conjugated with Alexa Fluor 647 (A21236) and donkey anti-goat IgG cojugated with Alexa Fluor 647 (A21447) all purchased from Life Sciences.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!