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Mouse anti glyr

Manufactured by Synaptic Systems
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Mouse anti-GlyR is a laboratory reagent used in research applications. It is an antibody that binds to the glycine receptor (GlyR) protein, which is a key component of inhibitory neurotransmission in the central nervous system. The antibody can be used to detect and study the expression and localization of the GlyR protein in biological samples.

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Lab products found in correlation

Prolong gold antifade reagent, by Thermo Fisher Scientific (2 mentions) Chicken anti gfp, by Aves Labs (2 mentions) Citrate buffer based antigen retrieval medium, by Biocare Medical (2 mentions) Rabbit anti gabaa receptor gamma 2 subunit, by Synaptic Systems (2 mentions) Guinea pig anti viaat, by Synaptic Systems (2 mentions) Decloaking chamber, by Biocare Medical (2 mentions) Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Dylight 488, by Jackson ImmunoResearch (2 mentions) Dylight 549, by Jackson ImmunoResearch (2 mentions) Dylight 649, by Jackson ImmunoResearch (2 mentions) Bca assay, by Thermo Fisher Scientific (1 mentions) Protease inhibitor, by Roche (1 mentions) Ecl reagent, by GE Healthcare (1 mentions) Lithium dodecyl sulfate (lds), by Thermo Fisher Scientific (1 mentions) Mouse anti β tubulin, by Merck Group (1 mentions) T per tissue protein extraction reagent, by Thermo Fisher Scientific (1 mentions) Nitrocellulose, by Bio-Rad (1 mentions) Mouse anti glyt2, by Merck Group (1 mentions)

3 protocols using mouse anti glyr

1

Immunostaining of Neuronal Markers in Tissue Sections

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Sections were cut at a thickness of 7 μm. After removing paraffin, sections were processed in a decloaking chamber (Biocare Medical, Concord, CA) using a citrate-buffer-based antigen retrieval medium (Biocare Medical) for 20 min at 110–115°C. They were then processed in PBS with 15% methanol and 0.3% H2O2 to block endogenous peroxidase activity. Aldehyde groups were removed by incubating the sections in sodium borohydride (1%) in PBS. After these treatments, the slices were incubated in a blocking PBS-based solution containing cold-water fish-skin gelatin (0.1%) and 0.1% Triton X-100. Tissues were then incubated overnight at 4°C with the following primary antibodies: chicken anti-GFP (1:1000 Aves labs, Tigard, OR), rabbit anti-GABAa receptor gamma 2 subunit (1:1500; Synaptic Systems, 224 003, Göttingen, Germany) mouse anti-GlyR (1:1000; Synaptic Systems, 146 011), guinea pig anti-VIAAT (1:1500; Synaptic Systems, data not shown). Primary antibodies were revealed by incubation for 2 h with secondary antibodies coupled to either Alexa Fluor-488 (Invitrogen, Saint Aubin, France) or DyLight 488, DyLight 549, and DyLight 649 (Jackson ImmunoResearch, Newmarket, UK). Sections were finally mounted using Prolong Gold Antifade Reagent (Invitrogen). For all experiments, control sections were incubated without primary antibodies.
Giber K., Diana M.A., Plattner V., Dugué G.P., Bokor H., Rousseau C.V., Maglóczky Z., Havas L., Hangya B., Wildner H., Zeilhofer H.U., Dieudonné S, & Acsády L. (2015). A subcortical inhibitory signal for behavioral arrest in the thalamus. Nature neuroscience, 18(4), 562-568.
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2

Immunostaining of Neuronal Markers in Tissue Sections

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Sections were cut at a thickness of 7 μm. After removing paraffin, sections were processed in a decloaking chamber (Biocare Medical, Concord, CA) using a citrate-buffer-based antigen retrieval medium (Biocare Medical) for 20 min at 110–115°C. They were then processed in PBS with 15% methanol and 0.3% H2O2 to block endogenous peroxidase activity. Aldehyde groups were removed by incubating the sections in sodium borohydride (1%) in PBS. After these treatments, the slices were incubated in a blocking PBS-based solution containing cold-water fish-skin gelatin (0.1%) and 0.1% Triton X-100. Tissues were then incubated overnight at 4°C with the following primary antibodies: chicken anti-GFP (1:1000 Aves labs, Tigard, OR), rabbit anti-GABAa receptor gamma 2 subunit (1:1500; Synaptic Systems, 224 003, Göttingen, Germany) mouse anti-GlyR (1:1000; Synaptic Systems, 146 011), guinea pig anti-VIAAT (1:1500; Synaptic Systems, data not shown). Primary antibodies were revealed by incubation for 2 h with secondary antibodies coupled to either Alexa Fluor-488 (Invitrogen, Saint Aubin, France) or DyLight 488, DyLight 549, and DyLight 649 (Jackson ImmunoResearch, Newmarket, UK). Sections were finally mounted using Prolong Gold Antifade Reagent (Invitrogen). For all experiments, control sections were incubated without primary antibodies.
Giber K., Diana M.A., Plattner V., Dugué G.P., Bokor H., Rousseau C.V., Maglóczky Z., Havas L., Hangya B., Wildner H., Zeilhofer H.U., Dieudonné S, & Acsády L. (2015). A subcortical inhibitory signal for behavioral arrest in the thalamus. Nature neuroscience, 18(4), 562-568.
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3

Spinal Cord Protein Analysis

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Spinal cord homogenates were prepared in T-PER tissue protein extraction reagent (ThermoFisher Scientific) containing protease inhibitors (Roche). Protein concentration was measured by BCA assay (ThermoFisher Scientific). Protein equivalents from each sample were boiled in LDS (ThermoFisher Scientific) containing 2.5% β-mercaptoethanol and electrophoresed on 4–12% Bis-Tris acetate gels (BioRad). Protein was transferred to nitrocellulose (BioRad) in transfer buffer containing 25 mM Tris, pH 8.3, 192 mM glycine, 0.1% (w/v) SDS, and 20% methanol. Membranes were blocked for 1 h in 20 mM Tris, 500 mM NaCl, pH 7.5 (TBS) containing 5% milk, then incubated overnight at 4 °C with primary antibodies diluted in TBS-0.1% tween-20 (TBST) containing 5% milk. After washing in TBST, membranes were incubated with HRP-conjugated goat-anti-rabbit or goat-anti-mouse antibodies (GE Life Sciences) and detected using ECL reagents (GE Life Sciences). Primary antibodies included rabbit anti-beta-galactosidase (1:500, MP/Cappel), mouse anti-beta-tubulin (1:1000, Sigma), rabbit anti-SLC7A10, N-term (1:1000, Acris, lot #FGI263), mouse anti-GLYR (1:1000, Synaptic Systems), rabbit anti-VIAAT (1:1000, Aviva), rabbit anti-GLYT1 (1:1000, Aviva), and mouse anti-GLYT2 (1:1000, Millipore). Densitometry was performed using ImageJ.
Ehmsen J.T., Liu Y., Wang Y., Paladugu N., Johnson A.E., Rothstein J.D., du Lac S., Mattson M.P, & Höke A. (2016). The astrocytic transporter SLC7A10 (Asc-1) mediates glycinergic inhibition of spinal cord motor neurons. Scientific Reports, 6, 35592.
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