20r cp003

Manufactured by Abcam

The 20R-CP003 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor and can accommodate various sample tubes or microplates. The centrifuge is capable of achieving a maximum speed of 6,000 RPM and a maximum relative centrifugal force (RCF) of 3,500 xg. This product is intended for use in research and diagnostic laboratories.

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Lab products found in correlation

Ab22378, by Abcam (3 mentions) Ab180117, by Abcam (2 mentions) Ab219799, by Abcam (2 mentions) 780 confocal microscope, by Zeiss (2 mentions) Axioscan, by Zeiss (2 mentions) Cd8 antibody, by Abcam (1 mentions) Foxp3 antibody, by Cell Signaling Technology (1 mentions)

3 protocols using 20r cp003

Quantification of Immune Infiltration in Mouse Tissues

Cited 1 time

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Briefly, tissues (tongue, cervical lymph nodes, and spleen) were harvested, fixed, and paraffin embedded. Slides were stained for CK5 (Fitzgerald, 20R-CP003) (1:500) and CD8 (abcam, ab22378) (1:400) antibodies. Quantification of immune infiltration was done using QuPath, an open source software for digital pathology image analysis^{60 (link)}. For the quantification, at least three regions of interest (ROI) were selected for each condition and the percentage of positive cells for the CD8 marker was calculated. In order to quantify the immune-fluorescent-stained Foxp3 and CD8 positive cells in the Foxp3^DTR mice, we quantified the number of positive cells in each ROI. CD8 antibody (catalog # ab22378) (1:400) was purchased from Abcam (Cambridge, United Kingdom) and FoxP3 antibody (catalog #D608R) (1:200) was purchased from Cell Signaling Technology (Danvers, MA).

Wang Z., Wu V.H., Allevato M.M., Gilardi M., He Y., Luis Callejas-Valera J., Vitale-Cross L., Martin D., Amornphimoltham P., Mcdermott J., Yung B.S., Goto Y., Molinolo A.A., Sharabi A.B., Cohen E.E., Chen Q., Lyons J.G., Alexandrov L.B, & Gutkind J.S. (2019). Syngeneic animal models of tobacco-associated oral cancer reveal the activity of in situ anti-CTLA-4. Nature Communications, 10, 5546.

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Immunological Profiling of Tumor Microenvironment

Cited 2 times

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Briefly, after the treatment with ICB, tissues (tongue, cervical lymph nodes, and spleen) were harvested, fixed, and paraffin embedded. Tissues were stained for Cytokeratin 5 (CK5, Fitzgerald, 20R-CP003) (1:500), CD8 (Abcam, ab22378) (1:400), anti-Syrian hamster IgG (Abcam, ab180117) and CD11c (Abcam, ab219799) antibodies. Secondary antibodies were used to reveal the specific marker signal in multiplex immune fluorescence (IF). Hematoxylin and eosin (H&E) staining was performed on tissue sections for histopathology analysis. Zeiss 780 confocal microscope was used for fluorescence imaging. Quantification of immune infiltration was performed using QuPath, an open-source software for digital pathology image analysis (12 (link)). Zeiss Axioscan was used to scan the H&E-stained sections. For the quantification, at least three regions of interest (ROI) were selected for each condition and the percentage of positive cells for each marker was calculated as we previously reported (13 (link)).

Gilardi M., Saddawi-Konefka R., Wu V.H., Lopez-Ramirez M.A., Wang Z., Soto F., Ramms D.J., Proietto M., Mikulski Z., Miki H., Sharabi A., Kupor D., Rueda R., Hollern D.P., Wang J, & Gutkind J.S. (2022). Microneedle-mediated intratumoral delivery of anti-CTLA-4 promotes cDC1-dependent eradication of oral squamous cell carcinoma with limited irAEs. Molecular cancer therapeutics, 21(4), 616-624.

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Quantifying Immune Infiltration in Treated Tissues

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Briefly, after the treatment with ICB, tissues (tongue, cervical lymph nodes, and spleen) were harvested, fixed, and paraffin embedded. Tissues were stained for Cytokeratin 5 (CK5, Fitzgerald, 20R-CP003) (1:500), CD8 (Abcam, ab22378) (1:400), anti-Syrian hamster IgG (Abcam, ab180117) and CD11c (Abcam, ab219799) antibodies. Secondary antibodies were used to reveal the specific marker signal in multiplex immune fluorescence (IF). Hematoxylin and eosin (H&E) staining was performed on tissue sections for histopathology analysis. Zeiss 780 confocal microscope was used for fluorescence imaging. Quantification of immune infiltration was performed using QuPath, an open-source software for digital pathology image analysis (12) (link). Zeiss Axioscan was used to scan the H&E-stained sections. For the quantification, at least three regions of interest (ROI) were selected for each condition and the percentage of positive cells for each marker was calculated as we previously reported (13) (link).

Gilardi M., Saddawi‐Konefka R., Wu V.H., Lopez‐Ramirez M.A., Wang Z., Soto F., Steffen D.J., Proietto M., Mikulski Z., Miki H., Sharabi A.B., Kupor D., Rueda R., Hollern D.P., Wang J., & Gutkind J.S. (2021). Microneedle-mediated intratumoral delivery of anti-CTLA-4 promotes cDC1-dependent eradication of oral squamous cell carcinoma with limited irAEs.

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