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Combur10test m stick

Manufactured by Roche
Sourced in Germany

The Combur10Test®M stick is a laboratory testing device used to detect the presence of various analytes in urine samples. It provides a quick and convenient way to perform basic urinalysis. The stick contains multiple test pads that can detect parameters such as pH, protein, glucose, ketones, bilirubin, and other urinary constituents. The test results are obtained by comparing the color changes on the test pads to a provided color chart.

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3 protocols using combur10test m stick

1

Urinalysis of Animals During Treatment

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Urinalysis was performed on all animals in the main groups during the 13th week of treatment and in the recovery groups during the 4th week of recovery. Freshly voided urine (urine voided within 3 h) was collected, and the following indicators were measured using a Combur10Test®M stick (Roche, Germany) and a urine chemistry analyzer (cobas u 411, Roche, Germany): pH, protein, glucose, ketone body, bilirubin, and occult blood. Urine color and transparency were examined macroscopically, and urine sediment was examined microscopically. Concentrated urine (urine voided within approximately 24 h) was collected to measure volume and specific gravity using a measuring cylinder and a refractometer (Vet360, Reichert, USA), respectively.
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2

Comprehensive Urinalysis of Rats

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Urinalysis was performed for 5 rats in each group on week 13 of administration. Fresh urine (sampled within approximately 3 hr of urine excretion) was collected to determine pH, protein, glucose, ketone body, bilirubin, and occult blood using a urine analyzer (Miditron® Junior II, Roche Diagnostics, Mannheim, Germany) or test kits (Combur 10 Test®M stick; Roche Diagnostics). Urine was also visually observed for color and turbidity and the sediment was examined microscopically (Olympus BX50, Olympus Optical Co., Tokyo, Japan). In addition, 24-hr urine (after excretion) samples were examined for urine volume, specific gravity, sodium, and potassium using a measuring cylinder, a specific gravimeter (Vet360, Reichert, Depew, NY, USA), or an automatic analyzer (AVL9181, Roche Diagnostics, Mannheim, Germany).
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3

Urinary Biomarker Evaluation in Rats

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On day 90, fresh urine was collected for 3 h from 5 rats of each sex per group in the fasting state, and a 21-h urine sample was subsequently collected while allowing access to food. Rats in metabolic cages (Biotoxtech, Cheongju, Republic of Korea) were allowed free access to water during both urine collection periods. The urine samples were stored at4°C until analysis was performed within 2 h of sample collection. The Combur10Test®M stick (Roche Diagnostics, Mannheim, Germany) was used to measure pH, protein, glucose, and occult blood, while urine chemistry was done with a Cobas u 411 urine analyzer (F. Hoffmann-La Roche, Basel, Swizerland). Urine color and turbidity were checked by visual inspection. The 24-h urine was used for measurement of the urine volume and specific gravity. The specific gravity was measured with a Gravimeter (VET 360, Reichert Technologies, New York, USA).
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