Acetaldehyde

Acetaldehyde and [ethyl-D₅]EtNH₃Cl were purchased from Millipore Sigma (St. Louis, MO, USA). In addition, 6-Chloropurine-2′-deoxyriboside was obtained from Carbosynth (Compton, UK). Water (LC-MS grade), methanol (MeOH, LC-MS grade), acetonitrile (ACN, LC-MS grade), 2-propanol (IPA, LC-MS grade), and formic acid (FA, 98% v/v) were purchased from Fisher Scientific (Hanover Park, IL, USA). Distilled water was purified by a Milli-Q system (Milford, MA, USA). Deoxyribonuclease I recombinant expressed by Pichia pastoris (R-DNase, 10,000 U/mg, phosphodiesterase-1 extracted from Crotalus adamanteus (PDE-1, 0.4 U/mg, recombinant alkaline phosphatase expressed by Pichia pastoris (R-ALP, 7000 U/mg, calf thymus DNA (CT-DNA, 5 mg), NaBH₃CN, Acetaldehyde, Tris base, double-filtration membrane Amicon Ultra (30 kDa cutoff, 0.5 mL), and single-filtration membrane Microcone (10 kDa cutoff, 0.5 mL) were purchased from Millipore Sigma (St. Louis, MO, USA). Silanized vials (0.3 mL, 1.2 mL, 4 mL, 20 mL) were purchased from ChromTech (Apple Valley, MN, USA). Cell lysis solution, protein precipitation solution, RNase A, and proteinase K were obtained from Qiagen (Hilden, Germany).

Reagent grade chemicals were purchased from Sigma-Aldrich (or as indicated): acetaldehyde (AA); acetate; acetylcholine chloride (ACh); Alda1 and cyanamide (gifts of Dr. B.G. Hill, University of Louisville); A967079 (AdooQ); 1h-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (ODQ); ethanol (100%); N^ω-nitro-L-arginine methyl ester hydrochloride (L-NAME); nicotine bitartrate; L-phenylephrine hydrochloride (PE); sodium nitroprusside (SNP); U46,619 (thromboxane A₂ analog); and 2,3,4,5-pentafluorobenzyl bromide (PFBBr).
Aortic Krebs PSS for aorta was (in mM): NaCl 118, KCl 4.7, CaCl₂ 2.5, KH₂PO₄ 1.2, MgSO₄ 1.2, NaHCO₃ 12.5, and glucose 5.5; pH 7.4. SMA Krebs physiological salt solution (PSS) was (in mM): NaCl, 119; KCl, 4.7; MgCl₂, 1.2; KH₂PO₄, 1.2; NaHCO₃, 24; glucose, 7.0; pH 7.4. High potassium (60 mM) PSS (High K⁺) was prepared by substituting K⁺ equimolar for Na⁺.

Pristine zeolite of faujasite-type structure denoted as HFAU (Si/Al = 31) was produced by Zeolyst company (CBV 760). It was dealuminated by steaming and acid treatment by the producer. Cu-containing zeolites Cu(2)HFAU and Cu(5)NaFAU were obtained by the impregnation method with 0.5 M Cu(NO₃)₂ solution. The amounts of Cu ions in solutions corresponded to 2% or 5% of Cu in impregnated zeolites. Zeolite Cu(2)HFAU was obtained by the impregnation of pristine HFAU. It contained 2 wt.% of Cu. In order to obtain Cu(5)NaFAU, zeolite HFAU was transformed into sodium form by four-fold exchange with 0.5 M NaNO₃, and then washed in distilled water. The resulting NaFAU was subsequently impregnated with 0.5 M Cu(NO₃)₂, producing zeolite containing 5 wt.% of Cu. All samples were dried at 390 K and next calcined at 770 K.
Bulk CuO and Cu₂O (Aldrich) were used in the IR measurements. Both oxides were mixed with SiO₂ Cabosil in proportion with 8 wt.% of oxides, and 92 wt.% of SiOEthanol, acetaldehyde and acetic acid (Aldrich) were used in IR adsorption experiments.