Diva v6

Manufactured by BD

Diva v6.1.2 is a laboratory equipment product from BD. It is a software tool designed for data analysis and visualization.

Automatically generated - may contain errors

Lab products found in correlation

Facs lsr 2, by BD (3 mentions) Special order facs aria 2 system, by BD (2 mentions) Fix and perm cell fixation and cell permeabilization kit, by Thermo Fisher Scientific (2 mentions) Alexa fluor 647 conjugate, by Thermo Fisher Scientific (2 mentions) Annexin 5 apc, by BD (2 mentions) Alexa fluor 647, by BD (2 mentions) Cd44 fitc, by Thermo Fisher Scientific (1 mentions) Epcam pe, by Miltenyi Biotec (1 mentions) Cd49f apc, by BioLegend (1 mentions) Epcam clone g8.8, by Thermo Fisher Scientific (1 mentions) Cd19 clone 1d3, by BD (1 mentions) Mac 1 clone m1 70, by BD (1 mentions)

5 protocols using diva v6

Quantifying Surface CXCR4 Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols

The non-treated and 10 ng/ml TNFα-treated OTICs were suspended in PBS, 0.5%BSA and stained with anti-CXCR4 antibody for 30 min at 4°C. After washing with PBS, cells were incubated with Alexa Fluor 594 anti-rabbit antibody for 30 min at 4°C. Fluorescence-activated cell sorting and analysis were performed on a BD Special Order FACS Aria II system and Diva v6.1.1 (BD Biosciences, San Jose, California). Live single cells were gated based on scatter properties and analyzed for their surface CXCR4 expression.

Yang-Hartwich Y., Gurrea-Soteras M., Sumi N., Joo W.D., Holmberg J.C., Craveiro V., Alvero A.B, & Mor G. (2014). Ovulation and extra-ovarian origin of ovarian cancer. Scientific Reports, 4, 6116.

+ Open protocol

+ Expand

Single Cell Surface Marker Isolation

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single cells were suspended in PBS, 2 mM EDTA, 0.5% BSA and stained with antibody for 15
min at 4°C. Fluorescence-activated cell sorting and analysis were performed on a BD Special
Order FACS Aria II system and Diva v6.1.1 (BD Biosciences). Live single cells were gated based on
scatter properties and analyzed for their surface marker expression as previously described
[15 (link)]. Antibodies used for FACS include Epcam-PE
(Miltenyi Biotech), CD44⁻FITC (ebioscience), and CD49f-APC (BioLegend).

Liu H., Cadaneanu R.M., Lai K., Zhang B., Huo L., An D.S., Li X., Lewis M.S, & Garraway I.P. (2015). Differential Gene Expression Profiling of Functionally and Developmentally Distinct Human Prostate Epithelial Populations. The Prostate, 75(7), 764-776.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

GFP, Annexin V (APC, BD Bioscience 550474), γH2AX (Alexa Fluor 647, BD Bioscience 560447), THY-1 (PE anti-human CD90 (Biolegend 328110)) and CD75 (Purified anti-human CD75 (LN-1, 326901 Biolegend) levels were analyzed with an LSR II FACS (BD Biosciences) using Diva v6.1.2 (BD Biosciences) and FlowJo software v10 (TreeStar). Cell permeabilization was performed using the Fix and Perm Cell fixation and cell permeabilization Kit (ThermoFisher Scientific, GAS003) following the manufacturer instructions. Goat anti-Mouse IgM Heavy Chain Secondary Antibody, Alexa Fluor® 647 conjugate (1:500) (A-21238, Thermo Fisher) was used as secondary antibody.

Stefano B.D., Ueda M., Sabri S., Brumbaugh J., Huebner A.J., Sahakyan A., Clement K., Clowers K.J., Erickson A.R., Shioda K., Gygi S.P., Gu H., Shioda T., Meissner A., Takashima Y., Plath K, & Hochedlinger K. (2018). Reduced MEK inhibition preserves genomic stability in naïve human ES cells. Nature methods, 15(9), 732-740.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

GFP and cell surface marker expression was analyzed with an LSR II FACS (BD Biosciences) using Diva v6.1.2 (BD Biosciences) and FlowJo software v10.2 (TreeStar). Primary antibodies used were CD19 (clone 1D3, BD Biosciences), MAC-1 (clone M1/70, BD Biosciences), SSEA1 (clone eBioMC-480, eBiosciences), THY1 (clone 53–2.1, eBiosciences), and EPCAM (clone G8.8, eBiosciences).

Brumbaugh J., Di Stefano B., Wang X., Borkent M., Forouzmand E., Clowers K.J., Ji F., Schwarz B.A., Kalocsay M., Elledge S.J., Chen Y., Sadreyev R.I., Gygi S.P., Hu G., Shi Y, & Hochedlinger K. (2017). Nudt21 controls cell fate by connecting alternative polyadenylation to chromatin signaling. Cell, 172(1-2), 106-120.e21.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!