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Wako labassay triglyceride kit

Manufactured by Fujifilm
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Sourced in Japan

The WAKO LabAssay Triglyceride Kit is a laboratory equipment product designed for the quantitative determination of triglycerides in biological samples. It provides a reliable and accurate method for measuring triglyceride levels.

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Pierce bca protein assay reagent, by Thermo Fisher Scientific (2 mentions) Pierce bca protein assay kit, by Thermo Fisher Scientific (2 mentions) Citrate assay kit, by Merck Group (1 mentions) Lactate assay kit, by Merck Group (1 mentions) Wako labassay cholesterol kit, by Fujifilm (1 mentions) Tissuelyser 2, by Qiagen (1 mentions) Free glycerol reagent, by Merck Group (1 mentions)

5 protocols using wako labassay triglyceride kit

1

Hepatic Lipid and Metabolite Analysis

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Flash frozen liver tissue (50 mg) was homogenized in a TissueLyser II (Qiagen Cat. No./ID: 85300). For TAG and cholesterol assays, liver homogenate was prepared as previously described.[25] The dried sample was resuspended in water before TAG and cholesterol analysis. Hepatic TAG (Wako LabAssay Triglyceride kit, Fuggerstraße, Neuss, Germany) and hepatic cholesterol (Wako LabAssay Cholesterol kit) was measured as per manufacturer's instructions. Hepatic citrate (Sigma‐Aldrich Citrate Assay Kit) and lactate (Sigma‐Aldrich Lactate Assay Kit) were measured as per manufacturer's instructions.
Mitchelson K.A., Tran T.T., Dillon E.T., Vlckova K., Harrison S.M., Ntemiri A., Cunningham K., Gibson I., Finucane F.M., O'Connor E.M., Roche H.M, & O'Toole P.W. (2022). Yeast β‐Glucan Improves Insulin Sensitivity and Hepatic Lipid Metabolism in Mice Humanized with Obese Type 2 Diabetic Gut Microbiota. Molecular Nutrition & Food Research, 66(22), 2100819.
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2

Quantifying Serum and Liver Triglycerides

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Measurement of serum TG was performed using the Wako LabAssay Triglyceride kit (290-63701) (Wako Chemicals USA). Liver TG was determined through the measurement of its glycerol contents38 (link). Briefly, liver tissue chunks were subjected to saponification in ethanolic KOH, and the released free glycerol from TG was measured with the Free Glycerol Reagent (F6428, Sigma-Aldrich).
Wei W., Chen Q., Liu M., Sheng Y., OuYang Q., Feng W., Yang X., Ding L., Su S., Zhang J., Fang L., Vidal-Puig A., Wang H.Y, & Chen S. (2022). TRIM24 is an insulin-responsive regulator of P-bodies. Nature Communications, 13, 3972.
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3

Adipocyte Differentiation and Triglyceride Quantification

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3T3-L1 cells were differentiated into adipocytes for 6 days in DMEM in the presence or absence of CG-1. Intracellular triglyceride levels were measured by using a WAKO LabAssay Triglyceride Kit (FUJIFILM Wako Pure Chemical, Osaka, Japan). Protein concentrations were determined by the use of a Pierce BCA Protein Assay Reagent (Thermo Fisher Scientific, Waltham, MA, USA). The intracellular triglyceride concentration was normalized to the protein concentration.
Matsumoto C., Koike A., Tanaka R, & Fujimori K. (2019). A Limonoid, 7-Deacetoxy-7-Oxogedunin (CG-1) from Andiroba (Carapa guianensis, Meliaceae) Lowers the Accumulation of Intracellular Lipids in Adipocytes via Suppression of IRS-1/Akt-Mediated Glucose Uptake and a Decrease in GLUT4 Expression. Molecules, 24(9), 1668.
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4

Triglyceride Quantification in Adipocytes

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3T3-L1 cells were differentiated into adipose cells for 6 days in DMEM containing baicalein or not. The triglyceride content in the cells as measured at day 6 past differentiation both in presence and absence of baicalein by using a WAKO LabAssay Triglyceride Kit (Wako Pure Chemical). Protein concentration was determined by using a Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA). Intracellular triglyceride level was adjusted with the protein concentration.
Nakao Y., Yoshihara H, & Fujimori K. (2016). Suppression of Very Early Stage Of Adipogenesis by Baicalein, a Plant-Derived Flavonoid through Reduced Akt-C/EBPα-GLUT4 Signaling-Mediated Glucose Uptake in 3T3-L1 Adipocytes. PLoS ONE, 11(9), e0163640.
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5

Intracellular Triglyceride Quantification

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Intracellular TG levels were measured by using a WAKO LabAssay Triglyceride Kit (Wako Pure Chemical, Osaka, Japan). Protein concentration was determined using a Pierce BCA Protein Assay Reagent (Thermo Fisher Scientific, Waltham, MA, USA) with bovine serum albumin to make the standard curve.
Nagai S., Matsumoto C., Shibano M, & Fujimori K. (2018). Suppression of Fatty Acid and Triglyceride Synthesis by the Flavonoid Orientin through Decrease of C/EBPδ Expression and Inhibition of PI3K/Akt-FOXO1 Signaling in Adipocytes. Nutrients, 10(2), 130.
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