Cd150 pe

Manufactured by Thermo Fisher Scientific

CD150-PE is a laboratory reagent that contains an antibody conjugated to the fluorescent dye phycoerythrin (PE). It is designed for use in flow cytometry applications to detect and analyze the expression of the CD150 cell surface marker.

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Spelling variants of this product

CD150 (5 citations) PE‐conjugated CD150 (2 citations) CD150-PE-Cy7 (2 citations) Anti-mouse CD150/SLAM PE antibody (2 citations) ), PE-anti-CD150 (2 citations)

2 protocols using cd150 pe

Multicolor Flow Cytometry Panel

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The following antibodies were used: mouse lineage cocktail-PE (BioLegend, cat# 78035), mouse lineage cocktail-APC (R&D Systems, cat# FLC001A), c-Kit-FITC (BioLegend, cat# 161603), c-Kit-APC (BioLegend, cat# 135108), c-Kit-PE/Cy7 (BioLegend, cat# 105814), c-Kit-BV421 (BioLegend, cat# 135124), Sca-1-BV605 (BioLegend, cat# 108133), Sca-1-APC (eBioscience, cat# 17-5981-82), Sca-1-BV421 (BioLegend, cat# 108127), Sca-1-PerCP-Cy5.5 (eBioscience, cat# 45-5981-82), CD150-BV421 (BioLegend, cat# 115925), CD150-PE (eBioscience, cat# 12-1501-82), CD150-PE-Cy7 (BioLegend, cat# 115913), CD48-PE/Cy7 (eBioscience, cat# 25-0481-80), CD48-BV711 (BioLegend, cat# 103439), CD48-APC-Cy7 (BioLegend, cat# 103432),CD34-FITC (eBioscience, cat# 11-0341-82), CD135-PE-Cy5 (BioLegend, cat# 135311), CD135-APC (BioLegend, cat# 135310), CD45.1-PerCP-Cy5.5 (BioLegend, cat# 110727), CD45.2-BV421 (BioLegend, cat# 109831), CD45.2-FITC(eBioscience, cat# 11-0454-82), Gr1-PE (BioLegend, cat# 108407), CD11b-APC (BioLegend cat# 101211), CD4-PE (BioLegend,cat# 116005), CD8a-PE (BioLegend, cat# 100707), B220-APC (BioLegend, cat# 103211).

Wang S., Han J., Huang J., Islam K., Shi Y., Zhou Y., Kim D., Zhou J., Lian Z., Liu Y, & Huang J. (2024). Deep learning-based predictive classification of functional subpopulations of hematopoietic stem cells and multipotent progenitors. Stem Cell Research & Therapy, 15, 74.

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Bone Marrow Cell Immunophenotyping Protocol

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Six weeks after tumour cell injection, mice were euthanized humanely. The femurs, tibias, and iliac crest were harvested immediately and placed in PBS. Bone marrow cells were obtained by rinsing femurs, tibias, and iliac crest with PBS. After collection, 1 × 10⁶–5 × 10⁶ cells were suspended in 100 μl of FACS buffer and stained with the appropriate antibodies at 4 °C. According to the manufacturer’s protocol, 10 μl of each antibody was sequentially added to the cell suspension on ice. The workflow is shown in Supplementary Figure 1. Fresh BM cells were stained with the following antibodies: lineage antibodies (B220 (RA3-6B2), CD3 (17A2), Gr-1 (RB6-8C5), CD11b (M1/70), CD8a (53–6.7), CD4 (GK1.5) and Ter-119 (TER-119)), Sca-1-Pe-Cy7 (D7, BD Biosciences), c-Kit-APC (2B8), CD150-PE (TC15-12F12.2), CD48-PerCP (HM48-1, eBioscience), CD41-FITC (MWReg30), CD34-FITC (RAM34, eBioscience), CD16/32-BV421 (93), CD71-FITC (C2, BD Biosciences), CD135-PE (A2F10) and CD138-PE (281-2).

Liu L., Yu Z., Cheng H., Mao X., Sui W., Deng S., Wei X., Lv J., Du C., Xu J., Huang W., Xia S., An G., Zhou W., Ma X., Cheng T., Qiu L, & Hao M. (2020). Multiple myeloma hinders erythropoiesis and causes anaemia owing to high levels of CCL3 in the bone marrow microenvironment. Scientific Reports, 10, 20508.

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