Pe anti mouse cd138 syndecan 1

Manufactured by BioLegend

PE anti-mouse CD138 (Syndecan-1) is a laboratory reagent used for the detection and analysis of mouse CD138 (Syndecan-1) expression on cell surfaces. It is a fluorescently labeled antibody that binds specifically to the CD138 protein.

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CD138-PE (14 citations) Anti-CD138 (14 citations) Anti-CD138-PE (6 citations) PE-anti-mouse CD138 (4 citations) CD138 (Syndecan-1 (2 citations)

3 protocols using pe anti mouse cd138 syndecan 1

Flow Cytometry Analysis of Murine Immune Cells

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Lymphocytes from blood, spleen, and bone marrow of immunized mice were harvested on day 7 after the final immunization and analyzed by flow cytometry. Dead cells were excluded by viability dye staining, and adherent cells were excluded by SSC/A and SSC/H gating analysis. Cells were analyzed by a BD LSRFortessa™ Flow Cytometry (BD Biosciences, USA). Data were acquired and analyzed by FlowJo version 10.5.2. LIVE/DEAD™ Fixable Dead Cell Stain Kits (Invitrogen) were used for viability dye staining. For surface staining, splenocytes were stained with the following antibodies: APC anti-mouse CD19 (Clone: 1D3/CD19, BioLegend), PE anti-mouse CD138 (Syndecan-1) (Clone: 281-2, BioLegend), FITC anti-mouse/rat/human CD27 (Clone: LG.3A10, BioLegend), PE anti-mouse CD279 (PD-1) (Clone: 29F.1A1, BioLegend), PE/Cyanine7 anti-mouse CD4 (Clone: GK1.5, BioLegend), and APC/Cyanine7 anti-mouse CD185 (CXCR5) (Clone: L138D7, BioLegend) for Tfh cell analysis; with FITC anti-mouse/human GL7 Antigen (Clone: GL7, BioLegend), PerCP/Cyanine5.5 anti-mouse CD95 (Fas), (Clone: SA367H8, BioLegend), and anti-B220-PerCP-Cy5.5 (Clone: RA3-6B2, BD Pharmingen™) mAb for GC B-cell analysis. Alexa Fluor 700 anti-mouse CD3 (Clone: 17A2, BioLegend), PE anti-CD8a-200 (Clone: 53-6.7, BioLegend), Brilliant Violet 605™ anti-mouse CD69 (Clone: H1.2F3, BioLegend), FITC anti-mouse CD107a (LAMP-1) (Clone: 1D4B, BioLegend).

Gao F., Huang J., Li T., Hu C., Shen M., Mu S., Luo F., Song S., Hao Y., Wang W., Han X., Qian C., Wang Y., Wu R., Li L., Li S, & Jin A. (2021). A Highly Conserved Peptide Vaccine Candidate Activates Both Humoral and Cellular Immunity Against SARS-CoV-2 Variant Strains. Frontiers in Immunology, 12, 789905.

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Immune Cell Dynamics in RBMRNA-405 Treatment

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RBMRNA-405-induced effects on proliferation and dynamics of immune cell populations were evaluated using Accuri C6 (BD Biosciences). For T-cell subset analysis, 1×10⁶ mice splenocytes (100 μl) were seeded per well into 96-well plates. Following two washes with PBS, the viability of cells was determined using Zombie Green™ Fixable Viability Kits (BioLegend, 423111). After washing once with FC buffer (PBS + 2% FBS), cells were resuspended in FC buffer. Then, splenocytes were stained with PerCP/Cyanine5.5 anti-mouse CD3 (BioLegend, 100218), PE anti-mouse CD8a (BioLegend, 100708), APC anti-mouse CD4 (BioLegend, 100412), PE anti-mouse CD62L (BioLegend, 161203), and APC anti-mouse/human CD44 (BioLegend, 103011). For GC response analysis, inguinal and iliac lymph nodes (LNs) were pooled and lymphocytes were isolated. Cells were treated with the same method as splenocytes except for the following antibodies: APC anti-mouse CD4 (BioLegend, 100412), PE anti-mouse CD185 (CXCR5) (BioLegend, 145503), PerCP/Cyanine5.5 anti-mouse CD278 (ICOS) (BioLegend, 117424), APC anti-mouse CD19 (BioLegend, 115512), PE anti-mouse CD95 (Fas) (BioLegend, 152608), PE anti-mouse CD138 (Syndecan-1) (BioLegend, 142504), APC anti-mouse/human CD45R/B220 (BioLegend, 103212), and PE anti-mouse CD79b (Igβ) (BioLegend, 132804).

Ma Q., Li M., Ma L., Zhang C., Zhang H., Zhong H., Wen J., Wang Y., Yan Z., Xiong W., Wu L., Guo J., Yang W., Yang Z, & Zhang B. (2023). SARS-CoV-2 bivalent mRNA vaccine with broad protection against variants of concern. Frontiers in Immunology, 14, 1195299.

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Isolation and Analysis of Mouse Immune Cells

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Spleens and bone marrow were harvested from mice and mashed first through a 70-μm and then a 40-μm nylon strainer (Sigma-Aldrich, St. Louis, MO) into RPMI medium to generate single-cell suspensions, which were then treated with ACK (Ammonium-Chloride-Potassium) lysis buffer (Lonza, Anaheim, CA) to remove red blood cells (RBCs). Cells (10 × 10⁶) were suspended in 0.2 mL PBS and incubated with PE anti-mouse CD138 (syndecan-1) (BioLegend, San Diego, CA) and APC Rat Anti-Mouse CD45R/B220 (clone RA3–6B2) (BD Biosciences, San Diego, CA) on ice in the dark room for 15 min. Cells were then washed twice with PBS and resuspended in 0.5 mL PBS; flow cytometric analysis was performed on a MACSQuant Analyzer 10 (BD Biosciences). A total of 1 × 10⁵ events were collected. FlowJo software (BD Biosciences) was used for data analysis.

Wang L., Kumar M., Deng Q., Wang X., Liu M., Gong Z., Zhang S., Ma X., Xu-Monette Z.Y., Xiao M., Yi Q., Young K.H., Ramos K.S, & Li Y. (2018). 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) Induces Peripheral Blood Abnormalities and Plasma Cell Neoplasms Resembling Multiple Myeloma in Mice. Cancer letters, 440-441, 135-144.

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