Analyst 800 system

Information about As concentrations in well water used by the mothers and their children was collected from HEALS WAs exposure database to verify lifetime consistent high or low WAs exposure in adolescent participants. Additionally, concurrent well WAs concentration for each mother and child was also obtained from this database. Laboratory analysis procedure for arsenic in drinking water has been described elsewhere (Cheng et al., 2004 (link); Van Geen et al., 2005 (link)). Concurrent UAs concentration was measured by graphite furnace atomic absorption spectrophotometry (GFAA), using a Perkin-Elmer Analyst 800 system as described (Nixon et al., 1991 (link)). All the samples were above the detection limit of 2 μg/L. Urinary creatinine (UCr) concentrations were analyzed by a colorimetric method based on Jaffe’s reaction (Heinegard and GunnarTiderstrom, 1973 ).

Urine samples were collected in our field clinic, frozen at 80°C, and shipped to Columbia University (CU) for a nalysis. UAs metabolite concentrations were measured in the CU Trace Metals Core Laboratory. Graphite furnace atomic absorption spectrophotometry (GFAA) via a Perkin-Elmer Analyst 800 system was used to assess total uAs concentrations.^{34 (link)} UAs metabolites were measured via HPLC separation of arsenobetaine, InAs^III, InAs^V, MMAs, and DMAs, and via ICP-MS-DRC.³⁵ In storage and analysis InAs^III can oxidize to InAs^V, so these species are reported jointly as total InAs.^{13 (link)}