Rat anti ph3

Manufactured by Abcam

Sourced in United Kingdom

Rat anti-pH3 is a primary antibody that specifically recognizes the phosphorylated form of histone H3. Histone H3 phosphorylation is a well-established marker of cell division and mitosis.

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Lab products found in correlation

Mouse anti mira, by Abcam (2 mentions) Chick anti gfp, by Abcam (2 mentions) Rabbit anti rfp, by Abcam (2 mentions) Alexa 568, by Thermo Fisher Scientific (1 mentions) Alexa650, by Thermo Fisher Scientific (1 mentions) Alexa505, by Thermo Fisher Scientific (1 mentions) Rabbit anti myc, by Santa Cruz Biotechnology (1 mentions) Rabbit anti apkc, by Santa Cruz Biotechnology (1 mentions) Alexa 488, by Thermo Fisher Scientific (1 mentions) Sp5 confocal microscope, by Leica camera (1 mentions) Rat anti mira, by Abcam (1 mentions) Chicken anti gfp, by Merck Group (1 mentions) Rabbit anti ph3, by Merck Group (1 mentions) Mouse anti mira, by Merck Group (1 mentions) Vectashield, by Clinisciences (1 mentions) Alexa fluor 488 donkey anti rabbit, by Thermo Fisher Scientific (1 mentions) Alexa fluor 594 goat anti rabbit, by Thermo Fisher Scientific (1 mentions) Mouse anti zo 1, by Thermo Fisher Scientific (1 mentions) Mouse anti γ tubulin, by Merck Group (1 mentions) Rabbit anti trfp, by Evrogen (1 mentions)

4 protocols using rat anti ph3

Immunostaining of Larval Drosophila Tissues

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Larval tissues were fixed for 20 min in 4% formaldehyde/phosphate-buffered saline (PBS) and immunostained as described (Bello et al. 2003 (link)). The primary antibodies used were rabbit anti-aPKC (1:500; Santa Cruz Biotechnology), mouse anti-Mira (1:50; gift of A. Gould), rat anti-pH3 (1:500; Abcam), chick anti-GFP (1:2000; Abcam), rabbit anti-Dpn (1:100; gift of Y.N. Jan), guinea pig anti-Dpn (1:1000; gift of James Skeath), rat anti-Pros (1:50; gift of F. Matsuzaki), rabbit anti-Ase (1:50; gift of F. Matsuzaki), rat anti-CycE (1:500; gift of Helena Richardson), mouse anti-CycA (1:50; Developmental Studies Hybridoma Bank), mouse or rat anti-Elav (1:100; Developmental Studies Hybridoma Bank), rabbit anti-Insc (1:1000; gift of W. Chia), rabbit anti-Myc (1:100; Santa Cruz Biotechnology), guinea pig anti-Nerfin-1 (1:5000; gift of A. Kuzin), rabbit anti-RFP (1:100; Abcam), and mouse anti-Fib (1:200; Abcam). Secondary goat antibodies conjugated to Alexa488, Alexa568, Alexa650, and Alexa505 (Molecular Probes) were used at 1:200. Images were collected on a Leica SP5 confocal microscope, and all images shown are single sections unless otherwise stated.

Froldi F., Szuperak M., Weng C.F., Shi W., Papenfuss A.T, & Cheng L.Y. (2015). The transcription factor Nerfin-1 prevents reversion of neurons into neural stem cells. Genes & Development, 29(2), 129-143.

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Immunostaining Larval and Pupal Brains

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Larval and pupal brains were dissected out in phosphate buffered saline (PBS), fixed for 20 min in 4% formaldehyde in PBS and rinsed in 0.2% PBST (PBS + 0.2% TritonX‐100). For immunostaining, brains were incubated with primary antibodies overnight at 4°C, followed by an overnight secondary antibody incubation at 4°C. Samples were mounted in 80% glycerol in PBS for image acquisition. The primary antibodies used were mouse anti‐Mira (1:50; gift of Alex Gould), rat anti‐Mira (1:100, Abcam), rat anti‐pH3 (1:500; Abcam), chick anti‐GFP (1:2000; Abcam), rabbit anti‐RFP (1:100, Abcam), mouse anti‐Repo (1:50, DSHB), guineapig anti‐Hth (1:100, Claude Desplan), mouse anti‐Ey (1:50, DSHB), guineapig anti‐Slp (1:500, Kuniaki Saito), rabbit anti‐D (1:1,000, Steve Russell), guineapig anti‐Tll (1:100, Kuniaki Saito), rabbit anti‐Tll (1:100, Kuniaki Saito) and guineapig anti‐Toy (1:50, Uwe Walldorf). Secondary donkey antibodies conjugated to Alexa 555 and Alexa 647, and goat antibody conjugated to Alexa 405, 488, 555 and 647 (Molecular Probes) were used at 1:500.

Veen K., Nguyen P., Froldi F., Dong Q., Alvarez‐Ochoa E., Harvey K.F., McMullen J.P., Marshall O., Jusuf P.R, & Cheng L.Y. (2023). Dedifferentiation‐derived neural stem cells exhibit perturbed temporal progression. EMBO Reports, 24(6), e55837.

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Immunostaining of Neural Progenitor Markers

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Dissected tissues were fixed 5 min or more in 4% formaldehyde/PBS depending on the primary antibody. Stainings were performed in 0.5% triton/PBS with antibody incubations separated by several washes. Tissues were then transferred in Vectashield (Clinisciences, France) with or without DAPI for image acquisition. Primary antibodies were: chicken anti-GFP (1:1000, Tebubio, France), mouse anti-Mira (1:50, A. Gould), guinea-pig anti-Mira (1:1000, A. Wodarz), guinea-pig anti-Asense (1:1000, J. Knoblich), rabbit anti-PH3 (1:500, Millipore, Billerica, MA), rat anti-PH3 (1:500, Abcam, UK), rat anti-Elav (1:50, DSHB, Iowa City, IA), rat anti-Chinmo (1:500, N. Sokol), rabbit anti-Castor (1:500, W. Odenwald), guinea-pig anti-Hunchback (1:500, J. Reinitz), guinea-pig anti-Kruppel (1:500, J. Reinitz), rabbit anti-Pdm (1:500, X. Yang), mouse anti-Svp (1:50, DSHB), rabbit anti-Imp (1:500, P. Macdonald), rat anti-Lin-28 (1:500, N. Sokol). Adequate combinations of secondary antibodies (Jackson ImmunoResearch, West Grove, PA) were used to reveal expression patterns.

Narbonne-Reveau K., Lanet E., Dillard C., Foppolo S., Chen C.H., Parrinello H., Rialle S., Sokol N.S, & Maurange C. (2016). Neural stem cell-encoded temporal patterning delineates an early window of malignant susceptibility in Drosophila. eLife, 5, e13463.

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Multicolor Immunostaining of Embryos

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Embryos were fixed in 4% paraformaldehyde (PFA) for 8-12 hr. Whole-mount staining was carried out according to previously described protocols (Norden et al., 2009) . Primary Antibodies Mouse anti-g-tubulin (Sigma-Aldrich; 1:250), rat anti-pH3 (Abcam; 1:500), rabbit anti-pH3 (Chemicon, 1:500), rabbit anti-tRFP (Evrogen, 1:500), and mouse anti-ZO1 (Invitrogen 1:200) were used. Secondary Antibodies Alexa Fluor 488 donkey antirabbit, Alexa Fluor 488 chicken antimouse, Alexa Fluor 568 goat antirat, Alexa Fluor 594 goat antirabbit, Alexa Fluor 647 goat antirat, Alexa Fluor 647 goat antirabbit, Alexa Fluor 647 goat antimouse (all Molecular Probes, all 1:1,000) were used.
Additionally, for detection of GFP and RFP fluorophores, GFP booster and RFP booster (ChromoTech) were used (1:400 dilution). Nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI) (1:5,000).

Strzyz P.J., Lee H.O., Sidhaye J., Weber I.P., Leung L.C, & Norden C. (2015). Interkinetic nuclear migration is centrosome independent and ensures apical cell division to maintain tissue integrity. Developmental cell, 32(2).

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