Dulbecco’s modified Eagle’s medium (DMEM) and heat-inactivated fetal bovine serum (FBS) were obtained from Fisher Scientific (Chicago, IL, USA). Penicillin and streptomycin were obtained from HyClone (Logan, UT, USA). Doxorubicin was obtained from Sigma (St. Louis, MO, USA). 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) was purchased from Thermo Fisher Scientific (Asheville, NC, USA). Rabbit monoclonal antibodies for PD-L1 (clone E1L3N®) were obtained from Cell Signaling Technology (Danvers, MA, USA), and monoclonal mouse anti-β-actin (clone AC-15) from Sigma (St. Louis, MO, USA). Secondary goat anti-rabbit immunoglobin G (IgG)-HRP (W401B) and goat anti-mouse IgG-HRP (W402B) monoclonal antibodies were purchased from Promega (Madison, WI, USA). Enhanced chemiluminescence reagents were obtained from Thermo Fisher Scientific (Asheville, NC, USA).
Rabbit monoclonal antibodies for pd l1 clone e1l3n
Manufactured by Cell Signaling Technology
Sourced in United States
Rabbit monoclonal antibodies for PD-L1 (clone E1L3N®) from Cell Signaling Technology are laboratory reagents designed for research use. They target the PD-L1 protein.
Automatically generated - may contain errors
Lab products found in correlation
Enhanced chemiluminescence reagent, by Thermo Fisher Scientific (2 mentions)
Doxorubicin, by Merck Group (2 mentions)
Mouse monoclonal anti β actin clone ac 15, by Merck Group (2 mentions)
Secondary goat anti rabbit immunoglobin g igg hrp w401b, by Promega (2 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Corning (1 mentions)
Heat inactivated fetal bovine serum, by Corning (1 mentions)
3 4 5 dimethyl 2 thiazolyl 2 5 diphenyltetrazolium bromide mtt, by Merck Group (1 mentions)
2 protocols using rabbit monoclonal antibodies for pd l1 clone e1l3n
1
Investigating Doxorubicin Cytotoxicity and PD-L1 Expression
2
Osteosarcoma Cell Line Characterization
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The SJSA-1 osteosarcoma cell line was purchased from the American Type Culture Collection (ATCC). Dulbecco's modified Eagle's medium (DMEM) and heat-inactivated fetal bovine serum (FBS) were obtained from Cellgro, whereas penicillin and streptomycin were obtained from HyClone. Doxorubicin and 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich. Rabbit monoclonal antibodies for PD-L1 (clone E1L3N) were obtained from Cell Signaling Technology, and monoclonal mouse anti–β-actin (clone AC-15) was from Sigma-Aldrich. Secondary goat anti-rabbit immunoglobin G (IgG)-HRP (W401B) and goat anti-mouse IgG-HRP (W402B) monoclonal antibodies were purchased from Promega. Enhanced chemiluminescence reagents were obtained from Thermo Fisher Scientific (32106).
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