The high-resolution analysis of GJ was performed with a liquid chromatography-mass spectrometry (LC-MS) system consisting of a Thermo Scientific Accela-HPLC System (composed of Accela 80 Hz PDA Detector (200 to 800 nm), Accela Autosampler, and Model 600 Pump) (Thermo Fisher Scientific, Waltham, MA, USA) and a Thermo Scientific LTQ Velos Mass Spectrometer System (Thermo Fisher Scientific, Waltham, MA, USA). The specific conditions of the analysis are noted in Table 1 .
Accela 80 hz pda detector
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Accela 80 Hz PDA detector is a high-speed photodiode array (PDA) detector designed for liquid chromatography (LC) applications. It operates at a sampling rate of up to 80 Hz, enabling fast data acquisition for improved peak resolution and accuracy in chromatographic analysis.
Automatically generated - may contain errors
Lab products found in correlation
Accela autosampler, by Thermo Fisher Scientific (3 mentions)
Accela 600 lc pump, by Thermo Fisher Scientific (2 mentions)
Accela hplc system, by Thermo Fisher Scientific (1 mentions)
Ltq velos mass spectrometer system, by Thermo Fisher Scientific (1 mentions)
Ptfe syringe filter, by Avantor (1 mentions)
3 protocols using accela 80 hz pda detector
1
High-Resolution LC-MS Analysis of GJ
2
Quantitative Analysis of Orange Juice Phenolics
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The major phenolic compounds present in orange juice were identified and quantified by high-performance liquid chromatography (HPLC-UV-MS), as described by Santos et al. [40 (link)]. The HPLC system consisted of a variable loop Accela autosampler (200 vial capacity set at 15 °C), an Accela 600 LC pump and an Accela 80 Hz PDA detector (Thermo Fisher Scientific, San Jose, CA, USA). The separation of orange juice compounds was carried out at 25 °C, with a gradient elution program at a flow rate of 0.2 mL/min. The mobile phase consisted of water/acetonitrile (90:10, v/v) (A) and acetonitrile (B), both with 0.1% of formic acid, applying the following linear gradient: 0–3 min, 0% B; 3–10 min, 0–10% B; 10–30 min, 10–20% B; 30–35 min, 20–25% B; 35–50 min, 25–50% B; 50–55 min, 50–100% B; followed by a ten min re-equilibration time before the next run. Before the injection, orange juice samples were filtered through a 0.2 µm PTFE syringe filter (VWR Internationa l, Radnor, PA, USA). Double online detection was carried out in the diode array detector, at 280 and 365 nm, and UV spectra in a range of 210–600 nm were also recorded.
3
UHPLC Analysis of Phytochemical Compounds
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The UHPLC system consisted of a variable loop Accela autosampler (200 vial capacity set at 15 °C), an Accela 600 LC pump and an Accela 80 Hz PDA detector (Thermo Fisher Scientific, San Jose, CA, USA). The separation of the compounds was carried out with a gradient elution program at a flow rate of 0.5 mL min À1 , at 45 °C, by using a Kinetex TM C 18 (50 mm  2.1 mm  1.7 lm) column supplied by Phenomenex (Thermo Fisher Scientific, San Jose, CA, USA). The injection volume in the UHPLC system was 6 lL and the mobile phase consisted in water:acetonitrile (99:1, v/v) (A) and acetonitrile (B), both with 0.1% of formic acid. The following linear gradient was applied: 0-4 min: 2%B, 4-7.5 min: 2-4. 5%B, 7.5-25.5 min: 4.5-11%B, 25.5-26 min: 11-12.8%B, 26-26.5 min: 12.8-30%B, 26.5-32 min: 30-39%B, 32-35 min: 39-47.5%B, 35-39 min: 47 .5-65%B, 39-44 min: 65-100%B, 44-48 min: 100-0%B, followed by re-equilibration of the column for 4 min before the next run. Double online detection was carried out in the diode array detector, at 280 and 340 nm, and UV spectra in a range of 200-600 nm were also recorded. Before the injection, each MeOH:H 2 O extract was dissolved in MeOH/H 2 O (50:50, v/v) HPLC grade, to obtain a final extract concentration of 30 mg mL À1 , and then filtered through a 0.2 lm PTFE syringe filter.
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