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Ergic 53 p58

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ERGIC-53/p58 is a lab equipment product that serves as a calcium-dependent lectin involved in protein trafficking between the endoplasmic reticulum and Golgi apparatus. It functions as a cargo receptor for the transport of glycoproteins.

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Lab products found in correlation

Sodium butyrate, by Merck Group (2 mentions) α tubulin, by Merck Group (2 mentions) Injectman ni2, by Eppendorf (2 mentions) Gm130, by BD (2 mentions) Tgn46, by Abcam (2 mentions) X tremegene 9 transfection reagent, by Roche (2 mentions) Puromycin, by Thermo Fisher Scientific (1 mentions) Polybrene, by Merck Group (1 mentions) Mouse monoclonal antibodies against flag and β actin, by Merck Group (1 mentions) Fugene and dual specific luciferase assay kit, by Promega (1 mentions) Recombinant ifn β, by R&D Systems (1 mentions) Hsv 1 kos strain, by National Collection of Authenticated Cell Cultures (1 mentions)

3 protocols using ergic 53 p58

1

Visualizing Golgi Apparatus Dynamics

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HeLa cells were microinjected with EspG proteins using a semiautomatic InjectMan NI2 (Eppendorf) with a needle concentration of 25 μM unless stated otherwise. Transfections were performed using XtremeGene 9 Transfection Reagent (Roche) for 16–18 hr. Expression of NAGT I in NAGFP cells was stimulated by the addition of 5 μM sodium butyrate (Sigma-Aldrich) to the media. AMCA-EspG was produced using an NHS-AMCA labeling kit (Pierce). BFA and nocodazole treatments were performed at 5 μg ml−1 for 30 min and 30 μM for 2 hr, respectively. Cellular markers were detected using following antibodies: GM130 (BD Transduction Labs), ERGIC-53/p58 (Sigma-Aldrich), TGN46 (Abcam), β-COP (EAGE, Joachim Seeman, UTSW), and α-tubulin (Sigma-Aldrich).
Selyunin A.S., Reddick L.E., Weigele B.A, & Alto N.M. (2014). Selective Protection of an ARF1-GTP Signaling Axis by a Bacterial Scaffold Induces Bidirectional Trafficking Arrest. Cell reports, 6(5), 878-891.
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2

Visualizing Golgi Apparatus Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols
HeLa cells were microinjected with EspG proteins using a semiautomatic InjectMan NI2 (Eppendorf) with a needle concentration of 25 μM unless stated otherwise. Transfections were performed using XtremeGene 9 Transfection Reagent (Roche) for 16–18 hr. Expression of NAGT I in NAGFP cells was stimulated by the addition of 5 μM sodium butyrate (Sigma-Aldrich) to the media. AMCA-EspG was produced using an NHS-AMCA labeling kit (Pierce). BFA and nocodazole treatments were performed at 5 μg ml−1 for 30 min and 30 μM for 2 hr, respectively. Cellular markers were detected using following antibodies: GM130 (BD Transduction Labs), ERGIC-53/p58 (Sigma-Aldrich), TGN46 (Abcam), β-COP (EAGE, Joachim Seeman, UTSW), and α-tubulin (Sigma-Aldrich).
Selyunin A.S., Reddick L.E., Weigele B.A, & Alto N.M. (2014). Selective Protection of an ARF1-GTP Signaling Axis by a Bacterial Scaffold Induces Bidirectional Trafficking Arrest. Cell reports, 6(5), 878-891.
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3

Innate Immune Sensing Mechanism Protocol

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3′3′-cGAMP, Lipofectamine 2000 (InvivoGen); poly(dA:dT), DNA90, ISD, HSV60, HSV120 (Sangon Biotech); polybrene (Millipore); SYBR (Bio-Rad); FuGene and Dual-Specific Luciferase Assay Kit (Promega); digitonin and VPS34-IN1 (Sigma); puromycin (Thermo); recombinant IFN-β (R&D Systems); ELISA kits for murine IFN-α and IFN-β (PBL) and IL-6 (Biolegend).
Mouse monoclonal antibodies against FLAG and β-actin (Sigma) and HA (Covance); rabbit monoclonal antibodies against phospho-TBK1, TBK1 and SNX8 (Abcam); MITA, VPS34, IRF3, phospho-IRF3, phospho-IκBα and phosphor-MITA (Ser366) (Cell Signaling Technology), rabbit polyclonal antibody against ERGIC-53/p58 (Sigma); Alexa Fluor 555 Mouse anti GM130 (BD Biosciences) were purchased from the indicated companies. Rabbit and mouse anti-SNX8 sera were raised against a recombinant human SNX8 protein.
HEK293 cells were provided by Dr. Gary Johnson (National Jewish Center). THP-1 cells and HeLa cells were purchased from ATCC. HFFs were provided by Dr. Minhua Luo (Wuhan Institute of Virology). HSV-1 (KOS strain) (China Center for Type Culture Collection, Wuhan, China), vaccinia virus (Tian-Tan Strain) (China Center for Type Culture Collection, Wuhan, China) and ECTV (Han-Zhong Wang, Wuhan Institute of Virology, Wuhan, China) viruses have been obtained from the indicated resources.
Wei J., Lian H., Guo W., Chen Y.D., Zhang X.N., Zang R., Zhong L., Yang Q., Hu M.M., Luo W.W., Shu H.B, & Li S. (2018). SNX8 modulates innate immune response to DNA virus by mediating trafficking and activation of MITA. PLoS Pathogens, 14(10), e1007336.
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