Tissues collected from the nude mice were fixed with 4% paraformaldehyde, embedded into paraffin, then stained with hematoxylin solution and eosin solution. After dehydration with graded ethanol, the slides were photographed using a Nanozoomer 2.0-RS fluorescence microscope (Hamamatsu, Japan).
Nanozoomer 2.0 rs fluorescence microscope
Manufactured by Hamamatsu Photonics
Sourced in Japan
The Nanozoomer 2.0-RS is a high-resolution fluorescence microscope designed for detailed imaging of biological samples. It utilizes a wide range of excitation and emission wavelengths to capture fluorescent signals from various fluorophores. The system provides high-speed scanning and superior image quality, making it a versatile tool for a variety of applications.
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Lab products found in correlation
3 protocols using nanozoomer 2.0 rs fluorescence microscope
1
Histological Analysis of Nude Mouse Tissues
2
Quantifying DNA Synthesis via Click-iT Assay
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DNA synthesis was quantified using a Click-iT™ EdU Imaging kit (Invitrogen; Carlsbad, CA, USA) according to the manufacturer's instruction. Briefly, cells were seeded in 96-well plates and 10 μM EdU was added to culture for 2 h. Next, cells were fixed with 4% paraformaldehyde for 15-30 min and permeabilized with 0.5% Triton X-100 for 20 min at room temperature. After washing with PBS, 100 μL Click-iT reaction mixture was incubated with the cells for 30 min, followed by 100 μL Hoechst 33342 in PBS for 30 min. The results were visualized using a NanoZoomer 2.0-RS fluorescence microscope (Hamamatsu, Japan).
3
Lung Metastasis Quantification
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Lungs collected from the nude mice were fixed with 4% paraformaldehyde for 24 h. After dehydration and paraffin embedding, the samples were sectioned at 4 µm thickness and stained with hematoxylin solution for 4 min. Then the sections were stained with eosin solution for 2 min and dehydrated with graded ethanol. The slides were mounted and photographed using a Nanozoomer 2.0-RS fluorescence microscope (Hamamatsu, Japan). The number of metastatic foci in the lungs was counted.
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