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Ultra 512x512 emccd camera

Manufactured by Oxford Instruments
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The ULTRA 512x512 EMCCD camera is a high-performance scientific imaging device. It features a 512x512 pixel sensor and utilizes Electron Multiplying CCD (EMCCD) technology to enable low-light imaging. The camera provides high quantum efficiency and low read noise, making it suitable for a range of scientific applications.

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Lab products found in correlation

Zen black, by Zeiss (1 mentions) Lsm 880 confocal laser scanning microscope, by Zeiss (1 mentions) Ti2e body, by Nikon (1 mentions) Plan apo λ 100 oil objective, by Nikon (1 mentions)

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EMCCD camera (203 citations) Ultra EMCCD camera (12 citations)

2 protocols using ultra 512x512 emccd camera

1

TIRF Microscopy for CD3 and ICAM-1 Quantification

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Total internal reflection fluorescence (TIRF) microscopy imaging was performed on an Olympus IX83 motorized TIRF microscopy with a 150×/1.45 NA objective and an Andor IXON ULTRA 512X512 EMCCD camera. For each cell the mean intensity of CD3 and ICAM-1 was calculated by dividing the total fluorescence intensity of the region of interest (ROI) minus background fluorescence of a neighbouring area (measured using the same ROI) and then divided by the area of the ROI.
Sanchez-Blanco C., Clarke F., Cornish G.H., Depoil D., Thompson S.J., Dai X., Rawlings D.J., Dustin M.L., Zamoyska R., Cope A.P, & Purvis H.A. (2018). Protein tyrosine phosphatase PTPN22 regulates LFA-1 dependent Th1 responses. Journal of Autoimmunity, 94, 45-55.
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2

Spinning Disk Confocal Microscopy Protocol

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All cells were imaged on a spinning disk confocal microscope except for Figure 3, which was on a Zeiss LSM 880 Confocal Laser Scanning Microscope equipped with a Plan-Apochromat 63× oil objective (1.4 N.A) and Airyscan detector and controlled by Zen Black (Zeiss). The spinning disk is a Nikon Ti2E body with PSF; CSU-X1 Yokogawa confocal scanner unit; OBIS 405, 488, 561, and 640 lasers and an ANDOR iXON Ultra 512X512 EMCCD camera. Images were acquired with Nikon Plan Apo λ 100× oil objective (1.4 N.A). Images were acquired with micro-manager 2.0. Images were processed in Fiji (NIH) and Adobe Illustrator (Adobe).
Nguyen T.T, & Voeltz G.K. (2022). An ER phospholipid hydrolase drives ER-associated mitochondrial constriction for fission and fusion. eLife, 11, e84279.
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