Anti cd19 fitc 6d5

Manufactured by BioLegend

Sourced in United States

Anti-CD19-FITC (6D5) is a fluorescently-labeled monoclonal antibody that targets the CD19 antigen. CD19 is a cell surface protein expressed on B cells and is commonly used as a marker for the identification and analysis of B lymphocytes.

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Lab products found in correlation

Facscalibur flow cytometer, by BD (1 mentions) Fvd efluor780, by Thermo Fisher Scientific (1 mentions) Facsdiva software, by BD (1 mentions) 7 aad, by BD (1 mentions) Anti foxp3 percp cy5.5 fjk 16 s, by Thermo Fisher Scientific (1 mentions) Annexin 5 pe, by BioLegend (1 mentions) Apc anti fas, by BioLegend (1 mentions) Streptavidin apc, by BioLegend (1 mentions)

Spelling variants of this product

Anti-CD19 (91 citations) CD19-FITC (53 citations) CD19 (6D5; (32 citations) Anti-CD19-FITC (30 citations) Anti-CD19 (6D5, (19 citations) FITC Cd19 (6D5, (2 citations)

2 protocols using anti cd19 fitc 6d5

Multiparametric Flow Cytometric Phenotyping

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Spleen cells were stained with fluorochrome-conjugated antibodies and analysed with a FACSCalibur flow cytometer and FACSDiva software (BD Biosciences, Oxford, UK). Cells were stained with the following antibodies: anti-Ter119-PE-Cy7 (TER-119; eBioscience, Inc., San Diego, CA, USA), anti-F4/80–fluorescein isothiocyanate (FITC; CI:A3-1), anti-CD11b-PE-Cy7 (M1/70), anti-CD11c-APC (N418), anti-CD4-APC (GK1.5), anti-CD8-PB (53-6.7), anti-CD19-FITC (6D5) (all from BioLegend, San Diego, CA, USA), and anti-Ki67-SAV (SoIA15; eBioscience). Dead cells were excluded with fixable viability dye (FVD)-eFluor 780 (eBioscience).

Schreiber T., Quinting T., Dittmer U., Fandrey J, & Sutter K. (2017). Hypoxia-inducible factor 1α is Essential for Macrophage-mediated Erythroblast Proliferation in Acute Friend Retrovirus Infection. Scientific Reports, 7, 17236.

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Multicolor Flow Cytometry for Lymphocyte Analysis

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Lymphocytes were stained with combinations of anti-CD4-Pacific Blue (GK1.5), anti-B220-FITC (RA3-6B2), anti-PD-1-PE or PE/Cy7 (RMP1-30), Streptavidin-APC, anti-CD69-PE/Cy7 (H1.2F3), anti-CD45-PE/Cy7 (30-F11), anti-CD19-FITC (6D5), anti-GL-7-PE or PerCP/Cy5.5 (GL7), anti-Fas-APC (SA367H8), anti-CD138-BV421 (281-2) anti-CD45.1-PE/Cy7 (A20), anti-IgM-PE (AF6-78), anti-IgD-Pacific Blue (11-26c.2a), anti-CD86-PE/Cy7 (GL-1), anti-CXCR4-PE (L276F12), anti-Bcl6-PE (7D1), and Annexin V-PE (Biolegend). Anti-CXCR5-biotin (2G8), anti-IgG1-APC (A85-1), and 7-AAD were purchased from BD Bioscience. Anti-Foxp3-PerCP/ Cy5.5 (FJK-16 s) was purchased from eBioscience. Anti-IL-4 (11B11), anti-IL-21 (mhalx21) antibodies, and intracellular staining kits (Biolegend) were used to detect intracellular cytokines according to the manufacturer's instructions. Samples were detected on a FACS Aria II. Data were analyzed using FlowJo V10 (TreeStar) software.

Qi H., Tian D., Li M., Zhang C., Jin H., Liu L., Zhao X., Ma L., Zhao W., Ge Q., Duan T, & Zhang D. (2020). Foxo3 Promotes the Differentiation and Function of Follicular Helper T Cells. Cell reports, 31(6).

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