The standard compounds, including cyanidin-3-O-galactoside (90% purity), cyanidin-3-O-glucoside (95% purity), cyanidin (95% purity), 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) (98% purity), 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) (98% purity), 2,9-dimethyl-1,10-phenanthroline (Neocuproine) (99% purity), potassium persulfate (99% purity), acetonitrile, formic acid, ethanol, methanol, and dimethyl sulfoxide (DMSO), were obtained from Sigma-Aldrich (Darmstadt, Germany). cyanidin-3-O-galactoside and cyanidin-3-O-arabinoside standards were bought from Polyphenols (Sandnes, Norway). HCl, NaNO2, H2O2, and CuCl2 were purchased from Merck (Darmstadt, Germany). Fetal bovine serum (FBS), Lonza, Dulbecco's Modified Eagle Medium (DMEM), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Lonza Group Ltd. (Basel, Switzerland). Glutamine, penicillin and streptomycin, and amphotericin were purchased from Sigma Chemical Co. (St. Louis, MO). The water used for experiments was treated in a Milli-Q water purification system.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
Manufactured by Lonza
Sourced in Switzerland, United States
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) is a colorimetric assay used to measure cell viability and proliferation. It is a tetrazolium-based compound that is reduced by metabolically active cells, converting the yellow MTT into purple formazan crystals.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Merck Group (2 mentions)
Cholesterol, by Biosynth (2 mentions)
Span 60, by Biosynth (2 mentions)
Dimethyl sulfoxide (dmso), by Serva Electrophoresis (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Lonza (2 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (2 mentions)
Acetonitrile, by Merck Group (2 mentions)
Cyanidin, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Amphotericin, by Merck Group (1 mentions)
Glutamine, by Merck Group (1 mentions)
Penicillin and streptomycin, by Merck Group (1 mentions)
Cyanidin 3 o galactoside, by Merck Group (1 mentions)
2 9 dimethyl 1 10 phenanthroline neocuproine, by Merck Group (1 mentions)
Nano2, by Merck Group (1 mentions)
Cyanidin 3 o glucoside, by Merck Group (1 mentions)
Ethanol, by Merck Group (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
5 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt
1
Standardized Compounds for Antioxidant Assays
2
Cytosolic Compounds Characterization
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The standard compounds—including Cy-3-O-samb-5-gluc chloride, Cy-3-O-samb chloride, Cy-3-O-gluc, and Cy—were purchased from Extrasynthese (Lyon, France). Water used for current experiments was treated in a Milli-Q water purification system. In Vitro Toxicology Assay Kit (LDH based TOX7), glutamine, penicillin, and streptomicin were purchased from Sigma Chemical Co. (St. Louis, MO, USA). Fetal bovine serum (FBS), Dulbecco’s Modified Eagle Medium (DMEM), and 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Lonza Group Ltd. (Basel, Switzerland). ApopTag Red In Situ Apoptosis Detection Kit was aquisitioned from Chemicon (Millipore, Bedford, MA, USA) and Draq5 was purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). Methanol (MeOH), ethyl acetate (EtOAc), formic acid, acetonitrile, hydrochloric acid (HCl), dimethylsulfoxide (DMSO), ethidium bromide/ acridine orange (EB/AO), and paraformaldehyde were bought from Merck (Darmstadt, Germany).
3
Geranium Oil-Mediated Cytotoxicity in MCF-7 Cells
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Cholesterol, Span 60, and
Tween 60 were acquired from Biosynth Carbosynth, Berkshire, UK. Geranium
oil was purchased from a local company (Imtenan, Cairo, Egypt). Ascorbic
acid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s
modified Eagle’s medium (DMEM) containing glucose (4.5 g/L),
trypsin (0.05%), and phosphate-buffered saline (PBS, pH 7.4), penicillin–streptomycin,
and 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT)
were purchased from Lonza Bioscience (Walkersville, MD, USA). Fetal
bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA). Dimethyl
sulfoxide (DMSO) was acquired from Serva (Heidelberg, Germany). 2′,7′-Dichlorodihydrofluorescein
diacetate (H2DCFDA) was purchased from Invitrogen-Thermo Fisher Scientific
(Waltham, USA), and an Annexin V-FITC/7-AAD apoptosis detection kit
(cat. no. E-CK-A212.50) was purchased from Elabscience (Wuhan, China).
Propidium iodide (PI) was purchased from Invitrogen (Waltham, MA,
USA). All other chemicals were purchased from Sigma-Aldrich. The MCF-7
cell line (cat. no. HTB-22) was purchased from ATCC (Manassas, VA,
USA).
Tween 60 were acquired from Biosynth Carbosynth, Berkshire, UK. Geranium
oil was purchased from a local company (Imtenan, Cairo, Egypt). Ascorbic
acid was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s
modified Eagle’s medium (DMEM) containing glucose (4.5 g/L),
trypsin (0.05%), and phosphate-buffered saline (PBS, pH 7.4), penicillin–streptomycin,
and 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT)
were purchased from Lonza Bioscience (Walkersville, MD, USA). Fetal
bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA). Dimethyl
sulfoxide (DMSO) was acquired from Serva (Heidelberg, Germany). 2′,7′-Dichlorodihydrofluorescein
diacetate (H2DCFDA) was purchased from Invitrogen-Thermo Fisher Scientific
(Waltham, USA), and an Annexin V-FITC/7-AAD apoptosis detection kit
(cat. no. E-CK-A212.50) was purchased from Elabscience (Wuhan, China).
Propidium iodide (PI) was purchased from Invitrogen (Waltham, MA,
USA). All other chemicals were purchased from Sigma-Aldrich. The MCF-7
cell line (cat. no. HTB-22) was purchased from ATCC (Manassas, VA,
USA).
4
Cholesterol Nanoparticle Cytotoxicity Assessment
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Cholesterol, Span 60, and Tween 60 were obtained from Biosynth Carbosynth, Berkshire, UK. Dulbecco’s modified Eagle’s medium (DMEM) with 4.5 g/L glucose, 0.05% Trypsin and phosphate-buffered saline (PBS, pH 7.4), Penicillin-streptomycin, and 3-(4,5-Dimethylthiazol-2-yl)-2,5 diphenyltetrazolium bromide (MTT) were purchased from Lonza Bioscience (Walkersville, MD, USA). Fetal bovine serum (FBS) was obtained from Gibco (Waltham, MA, USA). Dimethyl sulfoxide (DMSO) was obtained from Serva (Heidelberg, Germany). All other chemicals were purchased from Sigma-Aldrich, St. Louis, MO, USA.
5
Nanomaterial Synthesis and Cytotoxicity Assessment
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Hydrogen tetrachloroaurate(III) (HAuCl4*4H2O) was purchased from Merck (Darmstadt, Germany). Warfarin sodium (3-(α-Acetonylbenzyl)-4-hydroxycoumarin sodium salt) was purchased from Aldrich (St Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Lonza Group Ltd. (Basel, Switzerland). Kaighn’s Modification of Ham’s F-12 Medium was purchased from GIBCO (Cralsbad, CA, USA). The mixture of penicillin and streptomycin was purchased from Sigma Chemical Co. (St. Louis, MO, USA). Glutaraldehyde, OsO4, Epon 812 resin, and copper grids were purchased from Agar Sientific (Essex, UK); phosphate buffer components and acetone were purchased from Sigma.
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