Isoproterenol hydrochloride

Manufactured by Bio-Techne

Sourced in United Kingdom

Isoproterenol hydrochloride is a synthetic catecholamine compound. It acts as a non-selective beta-adrenergic agonist, stimulating both beta-1 and beta-2 adrenergic receptors.

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Lab products found in correlation

Forskolin, by Bio-Techne (1 mentions) Serotonin hydrochloride, by Bio-Techne (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) M csf, by Thermo Fisher Scientific (1 mentions) Hepes solution, by Thermo Fisher Scientific (1 mentions) Phorbol 12 myristate 13 acetate pma, by Bio-Techne (1 mentions) Ro 20 1724, by Merck Group (1 mentions) Dantrolene sodium salt, by Bio-Techne (1 mentions) Nkh 477, by Bio-Techne (1 mentions) Isoproterenol hydrochloride, by R&D Systems (1 mentions) 3 isobutyl 1 methylxanthine ibmx, by MP Biomedicals (1 mentions) Ym 254890, by Focus Biomolecules (1 mentions) Deferoxamine, by Merck Group (1 mentions) Sodium ascorbate, by Merck Group (1 mentions) Recombinant heregulinβ 1, by Thermo Fisher Scientific (1 mentions) Poly l lysine (pll), by Merck Group (1 mentions) Bafilomycin a1, by Merck Group (1 mentions) Forskolin, by Merck Group (1 mentions) Kt5720, by Merck Group (1 mentions) 2 2 bipyridyl, by Merck Group (1 mentions)

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Isoproterenol (30 citations)

5 protocols using isoproterenol hydrochloride

Macrophage Activation Signaling Compounds

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RPMI-1640 and penicillin-streptomyocin (P/S) from Invitrogen (Carlsbad, CA). Human AB serum and fetal bovine serum (FBS) from Mediatech (Tewksbury, MA). HEPES solution from Fisher Scientific (Fair Lawn, NJ). Dantrolene sodium salt, serotonin hydrochloride, isoproterenol hydrochloride, NKH 477, forskolin and phorbol 12-myristate 13-acetate (PMA) from Tocris Biosciences (Minneapolis, MN). isoproterenol hydrochloride from R&D Systems (Minneapolis MN). Dopamine and H89 dihydrochloride from Sigma-Aldrich (St. Louis, MO). 3-Isobutyl-1-methylxanthine (IBMX) from MP Biomedicals (Santa Ana, CA). RO-20-1724 from EMD Millipore (Temecula, CA). YM-254,890 from Focus Biomolecules (Plymouth Meeting, PA). Dopamine and the beta-adrenergic receptor agonist Isoproterenol were resuspended in distilled H₂O to make stock concentrations of 10 mM, then diluted into media as needed. RO-20-1724 (200 mM), IBMX (100 mM), forskolin (10 mM), YM-254,890 (10 mM) and PMA (100 μM) were resuspended in DMSO to the indicated concentrations, then diluted into media as indicated. Human macrophage colony stimulating factor (M-CSF) was from Peprotech (Rocky Hill, NJ) and resuspended in 100 μL distilled H₂O.

Nickoloff E.A., Mackie P., Runner K., Matt S.M., Khoshbouei H, & Gaskill P.J. (2019). Dopamine increases HIV entry into macrophages by increasing calcium release via an alternative signaling pathway. Brain, behavior, and immunity, 82, 239-252.

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Cyclic AMP and Neuregulin Regulation

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cAMP [8-(4-Chlorophenylthio) adenosine-3',5'-cyclic monophosphate] and AMP (8- Bromoadenosine-5-O-monophosphate) were purchased from Biolog (US distributor, Axxora LLC, San Diego, CA). Recombinant heregulin-β1 (herein referred to as neuregulin) was purchased from Peprotech (Rocky Hill, NJ). Forskolin, poly-l-lysine, bicarbonate, sodium ascorbate, bafilomycin A1, 2,2’-bipyridyl, deferoxamine and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane were purchased from Sigma-Aldrich (St. Louis, MO). Caffeine was bought from Enzo Life Sciences (Farmingdale, NY). SQ 22536, IBMX, ESI-09, CGRP (calcitonin gene-related peptide (rat)) and isoproterenol hydrochloride were purchased from Tocris Bioscience (Bristol, UK). KT5720 and H-89 Dihydrochloride were purchased from Millipore (Billerica, MA). LCD (L-cis-Diltiazem) was purchased from Abcam (Cambridge, UK). Dulbecco’s modified eagles’s medium (DMEM) that contain reduced level of sodium bicarbonate (1.5 g/L) (cat#30–2002) was purchased from American Type Culture Collection (ATCC) (Manassas, VA).

Camarena V., Sant D.W., Huff T.C., Mustafi S., Muir R.K., Aron A.T., Chang C.J., Renslo A.R., Monje P.V, & Wang G. (2017). cAMP signaling regulates DNA hydroxymethylation by augmenting the intracellular labile ferrous iron pool. eLife, 6, e29750.

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Optimization and Characterization of Isoproterenol Analogs

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The reagents used were as follows: Isoproterenol hydrochloride (Tocris Bioscience), dobutamine (Cayman chemical), sotalol (Cayman chemical), and DMSO (Focus Biomolecules). Reagents and solvents used in the synthesis of OptoIso, DiIsoprot, and MonoIsoprot were acquired from Oakwood Chemicals (reagents) or Sigma-Aldrich (solvents). According to the manufacturer’s instructions, all the reagents were dissolved in appropriate solvents and diluted in 1% Hank’s balanced salt solution supplemented with NaHCO₃, or a regular cell culture medium, before adding to cells.

Thotamune W., Ubeysinghe S., Shrestha K.K., Mostafa M.E., Young M.C, & Karunarathne A. (2024). Optical Control of Cell-Surface and Endomembrane-Exclusive β-Adrenergic Receptor Signaling. bioRxiv.

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Pharmacological Evaluation of Neurotransmitter Modulators

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Isoproterenol hydrochloride, tetrodotoxin citrate, and picrotoxin were purchased from Tocris Bioscience. Norepinephrine bitartrate salt monohydrate, methoxamine hydrochloride, and prazosin hydrochloride were purchased from Sigma-Aldrich. All experimental drugs were bath applied at their final concentrations as noted in the text. Dimethylsulfoxide (DMSO) was the solvent used for stock solution of picrotoxin where the maximum final concentration of DMSO in ACSF was 0.02% by volume. All other drugs, with the exception of prazosin, were easily dissolved in water. Prazosin was dissolved in water with vigorous heating and stirring.

Williams M.A., Li C., Kash T.L., Matthews R.T, & Winder D.G. (2014). Excitatory drive onto dopaminergic neurons in the rostral linear nucleus is enhanced by norepinephrine in an α1 adrenergic receptor-dependent manner. Neuropharmacology, 86, 116-124.

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Chemical Reagents for Metabolic Studies

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Rosiglitazone was purchased from LKT Laboratories (R5773), USA. Conjugated linoleic acid (1:1 ratio of c9,t11 and t10,c12) was purchased from Nu Chek Prep (UC-59-AX), USA. (R)-(-)-Phenylephrine hydrochloride and isoproterenol hydrochloride (isoprenaline) were purchased from Tocris Bioscience, UK.

Chai B.K., Lau Y.S., Loong B.J., Rais M.M., Ting K.N., Dharmani D.M, & Mohankumar S.K. (2018). Co-administration of conjugated linoleic acid and rosiglitazone increases atherogenic co-efficient and alters isoprenaline-induced vasodilatation in rats fed high fat diet. Physiological research, 67(5).

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