Rp 8 br pet cgmps

Netrin-1 (#1109-N1) was purchased from R&D Systems (Minneapolis, MN). Antibodies of proliferating cell nuclear antigen (PCNA/#sc-56) and Flk-1 (#sc-19530) were purchased from Santa Cruz (Dallas, TX). CD34 antibody (#ab8158) and GFP antibody (#ab290) were obtained from Abcam (Cambridge, MA), and Alexa Fluor 594 conjugate- Isolectin GS-IB4 antibody (#I21413) was purchased from Life Technologies (Carlsbad, CA). CD133 antibody (#orb10288) was obtained from Biorbyt (San Francisco, CA). P38 MAPK inhibitor SB202190 (#559388) and JNK inhibitor SP600125 (#420119) were purchased from EMD Millipore (Germany). ERK1/2 inhibitor U0126 (#U120), cGMP antagonist Rp-8-Br-PET-cGMPs (#B6684), NO scavenger 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), anti-von Willebrand Factor (vWF) antibody (#F3520), and anti-actin antibody (#A2066) were all purchased from Sigma. Antibody for p38 MAPK (#506123) was obtained from Calbiochem. Antibodies for phospho-p38 MAPK (#9211), alpha-Tublin (#2125), eNOS (#9586), and phospho-eNOS (Ser1179) (#9571) were all purchased from Cell Signaling Technology (Danvers, MA). Goat anti-rabbit IgG-HRP conjugate (#172–1019) was obtained from Bio-Rad (Irvine, CA). Other chemicals were obtained from Sigma in the highest purity.