Rna ish tissue assay kits

Manufactured by Thermo Fisher Scientific

The RNA ISH tissue assay kits are designed for the detection and visualization of RNA targets within fixed tissue sections. These kits utilize in situ hybridization techniques to enable the specific and sensitive localization of RNA transcripts in the tissue context.

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Lab products found in correlation

Tcs sp8 confocal system, by Leica (3 mentions)

Spelling variants of this product

RNA Assay Kit (15 citations) ISH kit (6 citations)

3 protocols using rna ish tissue assay kits

Brain Tissue Preparation and RNA ISH

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Example 11

Mice were euthanized then transcardially perused with PBS with heparin followed by 4% PFA. Brains were then removed and drop fixed in 4% PFA for 24 hours, frozen in OCT, and 12 μM sections were cut on a cryostat. Fluorescent in situ hybridization was performed using RNA ISH tissue assay kits (Affymetrix) following the manufacture's protocol. Tissues were treated with protease for 20 minutes at 40° C. 63× Images were acquired on a Leica TCS SP8 confocal system (Leica Microsystems) using the LAS AF Software.

US11389509B1. Methods for treatment of social dysfunction neurological disorders and seizures (2022-07-19). University of Virginia Patent Foundation [US]. Inventors: Anthony J. Filiano [US], Jonathan Kipnis [US].

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Fluorescent in situ Hybridization of Brain Tissue

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Mice were euthanized then transcardially perused with PBS with heparin followed by 4% PFA. Brains were then removed and drop fixed in 4% PFA for 24 hours, frozen in OCT, and 12μM sections were cut on a cryostat. Fluorescent in situ hybridization was performed using RNA ISH tissue assay kits (Affymetrix) following the manufacture’s protocol. Tissues were treated with protease for 20 minutes at 40°C. 63× Images were acquired on a Leica TCS SP8 confocal system (Leica Microsystems) using the LAS AF Software.

Filiano A.J., Xu Y., Tustison N.J., Marsh R.L., Baker W., Smirnov I., Overall C.C., Gadani S.P., Turner S.D., Weng Z., Peerzade S.N., Chen H., Lee K.S., Scott M.M., Beenhakker M.P., Litvak V, & Kipnis J. (2016). Unexpected role of interferon-γ in regulating neuronal connectivity and social behavior. Nature, 535(7612), 425-429.

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Fluorescent in situ Hybridization of Brain Tissue

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Mice were euthanized then transcardially perused with PBS with heparin followed by 4% PFA. Brains were then removed and drop fixed in 4% PFA for 24 hours, frozen in OCT, and 12μM sections were cut on a cryostat. Fluorescent in situ hybridization was performed using RNA ISH tissue assay kits (Affymetrix) following the manufacture’s protocol. Tissues were treated with protease for 20 minutes at 40°C. 63× Images were acquired on a Leica TCS SP8 confocal system (Leica Microsystems) using the LAS AF Software.

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