Ly6g bv421

Manufactured by Thermo Fisher Scientific

The Ly6G-BV421 is a fluorescent-labeled antibody that specifically binds to the Ly6G surface antigen, which is expressed on mature neutrophils. This product can be used for the identification and analysis of neutrophils in various research applications.

Automatically generated - may contain errors

Lab products found in correlation

Cd9 exo flow capture beads, by System Biosciences (1 mentions) Cd326 pe, by Thermo Fisher Scientific (1 mentions) F4 80 apc, by Thermo Fisher Scientific (1 mentions) Cd31 fitc, by Thermo Fisher Scientific (1 mentions) Mhcii apc efluor 780, by Thermo Fisher Scientific (1 mentions) Cd19 bv421, by BioLegend (1 mentions) Cd19 apch7, by Thermo Fisher Scientific (1 mentions) Cd11b pe cy7, by Miltenyi Biotec (1 mentions) Ly6c apc, by Miltenyi Biotec (1 mentions) Facscanto 2, by BD (1 mentions) Facsdiva version 8, by BD (1 mentions) Cd11c pe cy7, by Thermo Fisher Scientific (1 mentions) Siglecf pe, by BD (1 mentions) Cd11b bv510, by BioLegend (1 mentions) Cd45 percp, by BioLegend (1 mentions) Nk1.1 pe, by Thermo Fisher Scientific (1 mentions) F4 80, by BioLegend (1 mentions)

Spelling variants of this product

Anti-LY6G-BV421 antibody (3 citations) Anti-Ly6G-BV421 (2 citations)

2 protocols using ly6g bv421

Phenotyping Extracellular Vesicles by Flow

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cellular source of EVs was determined by flow cytometry using CD9 Exo-Flow capture beads (System Biosciences; Palo Alto, CA, USA) as per manufacturers' instructions. EVs were attached to CD9 labeled Exo-Flow beads at 4°C, for 16 h, and stained with F4/80-APC, CD31-FITC, CD326-PE, and Ly6G-BV421 (eBioscience) antibodies, and analyzed on BD LSR Fortessa Flow Cytometer at OSU Flow cytometry core facility. Data were analyzed using FlowJo.

Nirujogi T.S., Kotha S.R., Chung S., Reader B.F., Yenigalla A., Zhang L., Shapiro J.P., Wisler J., Christman J.W., Maddipati K., Parinandi N.L, & Karpurapu M. (2022). Lipidomic Profiling of Bronchoalveolar Lavage Fluid Extracellular Vesicles Indicates Their Involvement in Lipopolysaccharide-Induced Acute Lung Injury. Journal of Innate Immunity, 14(5), 555-568.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

Heparinized whole blood was used for flow cytometry using the following antibody cocktail: CD45-PerCP (Clone 30-F11; Biolegend 103130), CD3-eFLUO450 (Clone 17A2; eBioscience 48-0032), NK1.1-PE (Clone PK136; BD557391), Ly6G-APC-CY7 (Clone 1A8; BD560600), CD11b PE-CY7 (Clone M1/70; BD552850), Ly6C-APC (Clone 1G7.G10; Miltenyi 130-093-136), CD19-APC-H7 (Clone 1D3, eBioscience 47-0193) and Siglec-F-PE (Clone E50-2440; BD552126). Further, cells from the whole brain were isolated for FACS. Anesthetized mice were perfused with PBS to remove the peripheral blood. Subsequently, brains were dissected, mechanically dissociated and digested with a collagenase mix (including collagenase IX, collagenase I, DNAse and RPMI-HEPES). Immune cells were separated using a Percoll-gradient and stained with CD45 PerCP (Clone 30-F11; Biolegend 103130), CD11c PE-Cy7 (Clone N418, eBioscience 25-0114)), F4/80 (Clone BM8; Biolegend 123116), CD11b BV510 (Clone M1/70; Biolegend 101245), CD3 BV421 (Clone 17A2; eBioscience 48-0032-82), CD19 BV421 (Clone 6D5; Biolegend 115538), Ly6G BV421 (Clone eBio927; eBioscience 48-3172), MHC II-APC-eFluor780 (Clone M5/114.15.2; eBioscience 47-5321-82). All samples were measured with a FACS-Canto II (BD Biosciences). Results were analysed with FACSdiva version 8 (BD Biosciences).

Kerkhofs D., van Hagen B.T., Milanova I.V., Schell K.J., van Essen H., Wijnands E., Goossens P., Blankesteijn W.M., Unger T., Prickaerts J., Biessen E.A., van Oostenbrugge R.J, & Foulquier S. (2020). Pharmacological depletion of microglia and perivascular macrophages prevents Vascular Cognitive Impairment in Ang II-induced hypertension. Theranostics, 10(21), 9512-9527.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!