Cell lines were purchased from Japan Health Science Research Resource Bank (IM95, KATOIII, MKN1, NUGC4, and OCUM1) and Korean Cell Line Bank (NCC19, NCC24, NCC59, SNU719, SNU484, SNU1750, and SNU1967). YCC6 and YCC21 cells were gifts from Yonsei University College of Medicine, Seoul. Cell lines were authenticated using Short Tandem Repeat profiling using ANSI/ATCC ASN-0002-2011 guidelines and tested Mycoplasma negative according to the MycoAlert Mycoplasma Detection Kit (Lonza). All cell lines used in this study were maintained in a 37 °C incubator, 5% CO2, and propagated in media containing 10% FBS and 1% NEAA in IM95—DMEM with 10 mg/L insulin, OCUM1—DMEM with 0.5 mM Na-Pyruvate, MKN1, NCC19, NCC24, NCC59, SNU484, SNU719, SNU1750, and SNU1967—RPMI.
Ncc24
Manufactured by Korean Cell Line Bank
The NCC24 is a laboratory instrument designed for the cultivation and maintenance of cell lines. It provides a controlled environment for the growth and proliferation of various cell types. The NCC24 maintains consistent temperature, humidity, and gas composition to support the optimal conditions required for cell culture.
Automatically generated - may contain errors
Lab products found in correlation
Snu 719, by Korean Cell Line Bank (6 mentions)
Ncc19, by Korean Cell Line Bank (2 mentions)
Ncc59, by Korean Cell Line Bank (2 mentions)
Mycoalert mycoplasma detection kit, by Lonza (2 mentions)
Snu 484, by Korean Cell Line Bank (2 mentions)
Nugc 3, by American Type Culture Collection (2 mentions)
Mkn28, by Korean Cell Line Bank (2 mentions)
Rpmi 1640, by Thermo Fisher Scientific (2 mentions)
Kato 3, by Korean Cell Line Bank (1 mentions)
Ocum1, by Korean Cell Line Bank (1 mentions)
Snu 16, by Korean Cell Line Bank (1 mentions)
Snu1750, by Korean Cell Line Bank (1 mentions)
Snu 601, by Korean Cell Line Bank (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Nci n87, by Korean Cell Line Bank (1 mentions)
Fetal bovine serum (fbs), by Corning (1 mentions)
Rpmi 1640 medium, by Nacalai Tesque (1 mentions)
Namalwa, by American Type Culture Collection (1 mentions)
8 protocols using ncc24
1
Gastric Cancer Cell Line Authentication
2
Gastric Cancer Cell Line Characterization
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Cell lines were purchased from Japan Health Science Research Resource Bank (IM95, NUGC3, and MKN1), ATCC (SNU16, AGS) and Korean Cell Line Bank (NCC59, NCC24, SNU1967, SNU719, SNU484, NCC19, and SNU1750). YCC10 and YCC11 (Yonsei Cancer Centre in Seoul, South Korea), GES1 (Dr. Alfred Cheng, Chinese University of Hong Kong), and HFE145 (Dr. Hassan Ashktorab, Howard University) were kind gifts. Cell lines were authenticated using Short Tandem Repeat profiling using ANSI/ATCC ASN-0002-2011 guidelines and tested Mycoplasma negative according to the MycoAlert Mycoplasma Detection Kit (Lonza). Normal human stomach antrum and fundus tissue slides were purchased from Novus Biologicals (NBP2-30203, NBP2-30204). Human tissue microarray slides containing matched normal and gastric cancer cases, and human control tissue sections (liver and muscle) were provided and processed by SingHealth Advanced Molecular Pathology Laboratory, Singapore.
3
Gastric Cancer Cell Lines Cultivation
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The gastric cancer cell line AGS was obtained from the American Type Culture Collection (Manassas, VA, USA). EBV (−) GC cell lines (SNU-601, MKN-1, and MKN-28) and EBV (+) GC cell lines (SNU-719 and NCC-24) were obtained from the Korean Cell Line Bank (Seoul, Korea). The six cell lines were cultured in RPMI1640 medium. The EBV (+) YCCEL1 GC cell line was a kind gift from Dr. SY Rha from the Yonsei University College of Medicine. YCCEL1 cells were cultured in minimum essential medium (MEM). Media were supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, USA) and 1% penicillin/streptomycin (Life Technologies, Carlsbad, CA, USA). Cultures were maintained at 37 °C in a humidified atmosphere containing 5% CO2. Informed consent was obtained from all patients and the experimental protocol was reviewed and approved by the Tissue Ethical Committee of Korea University Ansan Hospital for the use of tissue specimens (No. 2016-004). The method was carried out in accordance with the committee’s approved guidelines. Paraffin-embedded GC and non-malignant gastric tissue specimens were used for immunohistochemistry.
4
Gastric Cancer Cell Line Culturing and Characterization
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AGS, SNU-719, MKN-1, MKN-28, NCI-N87, SNU484, and NCC24 cells were purchased from Korean Cell Line Bank (Seoul, Korea). YCCEL1 cells were given by professor Sun Young Rha (Yonsei University College of Medicine, Republic of Korea). AGS-EBV cells were given by Takada K (Institute for Genetic Medicine, Hokkaido University, Japan). The GC cell lines were cultured in RPMI 1640 (Gibco, Grand Island, NY, USA) containing 10% fetal bovine serum (FBS, Corning, NY, USA), 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco) except for YCCEL1 which was cultured in Eagle’s minimal essential medium (Lonza Benelux BV, Breda, the Netherlands). AGS-EBV is an AGS cell line infected with a recombinant Akata strain of EBV [38 (link)]. To culture AGS-EBV cells, 400 μg/mL of G418 (Gibco) was added to the medium. SNU-719 [39 (link)], YCCEL1 [40 (link)], and NCC-24 [41 (link)] are gastric carcinoma cell lines naturally infected with EBV. The human embryonic kidney cell line HEK293T was cultured in Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin (Gibco). All cells were incubated at 37 °C and supplemented with 5% CO2.
5
Culturing Lymphoma and Leukemia Cell Lines
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The Burkitt lymphoma cell line Namalwa (CRL-1432) was purchased from American Type Culture Collection (Manassas, VA, USA). The EBVaGC cell lines SNU-719 and NCC-24 were purchased from Korean cell line bank (Seoul, Korea). The EBV-negative acute monocytic leukemia cell line THP-1 (with diploid chromosomes) was obtained from Cell Resource Center for Biomedical Research, Institute of Development, Aging and Cancer, Tohoku University (Sendai, Japan). Cell lines were cultured in RPMI 1640 medium (Nacalai Tesque, Kyoto, Japan) containing 10% fetal bovine serum and 1% penicillin and streptomycin.
6
Gastric Cancer Cell Line Culture and Manipulation
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The GC cell line SNU-216,HGC-27 and SNU-1 were purchased from American Type Culture Collection(ATCC), and NCC-24 was purchased from Korean Cell Line Bank. The GC cells were cultured in RPMI-1640 (Gibco,C11875500BT) with 10% fetal bovine serum, penicillin (100U/ml), and streptomycin (100 g/ml) in a humidated incubator with 5% CO2 at 37 ℃. Lentiviral shRNA and overexpression vectors targeting VCAM1 were purchased from GeneChem(Shanghai,China).The inhibitors of STAT3 phosphorylation(MCE,HY-13818) was added in culture medium to stimulate GC cells for one day.
7
Culturing EBV-Positive & MSS GC Cell Lines
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The EBV-positive GC cell lines SNU-719 and NCC24 were purchased from the Korean Cell Line Bank (Seoul, Korea). AGS cell line was cultured to represent microsatellite stable (MSS)/EBV-negative GC cell line. All three cell lines were cultured in RPMI 1640 medium containing 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin. Cell lines were maintained and passaged for up to 12 months.
8
Cell Line Cultivation for EBV-Associated Gastric Cancer
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The EBVaGC cell lines SNU719 and NCC24 were obtained from the Korea Cell Line Bank (Seoul, South Korea), and the YCCEL1 cell line was obtained from Prof. SY Rha from the Yonsei Cancer Center [22] [23] [24] . The EBV-negative gastric cancer cell lines AGS and NUGC3 and the T-cell lymphoma cell line Jurkat were obtained from ATCC.
The SNU719, AGS, NUGC3, and Jurkat cells were cultured in RPMI-1640; 25 mM HEPES and 25 mM NaHCO3 were added to the RPMI-1640 for NCC24. YCCEL1 was cultured in MEM. All cultures were supplemented with 10% heat-inactivated FBS (Sigma-Aldrich, St. Louis, MO, USA) and cultured at 37 °C in a humidified incubator with 5% CO 2 .
The SNU719, AGS, NUGC3, and Jurkat cells were cultured in RPMI-1640; 25 mM HEPES and 25 mM NaHCO3 were added to the RPMI-1640 for NCC24. YCCEL1 was cultured in MEM. All cultures were supplemented with 10% heat-inactivated FBS (Sigma-Aldrich, St. Louis, MO, USA) and cultured at 37 °C in a humidified incubator with 5% CO 2 .
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