6545 quadrupole time of flight

Manufactured by Agilent Technologies

The 6545 quadrupole time-of-flight is a high-resolution mass spectrometry instrument designed for accurate mass measurements and compound identification. It combines a quadrupole mass analyzer with a time-of-flight analyzer to provide high-resolution, accurate mass data.

Automatically generated - may contain errors

Lab products found in correlation

Spectrum 400 ftir instrument, by PerkinElmer (1 mentions) 1290 infinity 2 hplc, by Agilent Technologies (1 mentions) Eclipse plus c18, by Agilent Technologies (1 mentions) Agilent 1290 infinity 2 uhplc system, by Agilent Technologies (1 mentions) Dual jet stream, by Agilent Technologies (1 mentions)

5 protocols using 6545 quadrupole time of flight

Detailed Spectroscopic Analysis of Organic Compounds

Check if the same lab product or an alternative is used in the 5 most similar protocols

All chemicals and reagents were purchased from available
commercial
companies and were used without further purification. Reactions were
monitored by thin-layer chromatography using pre-coated aluminum sheets
(silica gel 60 F₂₅₄, 0.2 mm thickness). Formation of the
desired product was confirmed by infrared (IR), ¹H NMR, ¹³C NMR, and mass spectra (LC–MS/HRMS). IR spectra were
recorded on a Perkin Elmer Spectrum 400 FTIR instrument, and the frequencies
are expressed in cm^–1. ¹H NMR and ¹³C NMR spectra were recorded on a Bruker Avance II-400 spectrometer
in CDCl₃, and chemical shifts were recorded in ppm with
TMS as the internal standard. The HMBC spectrum was recorded on an
ECZR series 600 MHz NMR spectrometer (Jeol, Japan). Melting points
were recorded by an open capillary tube method. Mass spectral data
were obtained with a Waters UPLC-TQD mass spectrometer (ESI-MS). High-resolution
mass spectra (ESI-HRMS) were recorded on an Agilent 6545 Quadrupole
Time-of-Flight. All reactions were purified by column chromatography
over silica gel (100–200 mesh) using ethyl acetate and hexane
as the eluent.

Marpna I.D., Shangpliang O.R., Wanniang K., Kshiar B., Lipon T.M., Laloo B.M, & Myrboh B. (2021). Trifluoroacetic Acid-Mediated Oxidative Self-Condensation of Acetophenones in the Presence of SeO2: A Serendipitous Approach for the Synthesis of Fused [1,3]Dioxolo[4,5-d][1,3]dioxoles. ACS Omega, 6(22), 14518-14524.

+ Open protocol

+ Expand

PFAS Quantitation by LC-MS/QTOF

Check if the same lab product or an alternative is used in the 5 most similar protocols

LC-MS analysis was performed using an Agilent 1290 Infinity II HPLC coupled to an Agilent 6545 quadrupole time-of-flight (QTOF) mass spectrometer. Details of the analytical method are described in the Supporting Information (Text S1). Authentic standards were used to determine all PFAS concentrations. When possible, quantification was conducted with an isotope dilution approach, in which the analyte response was normalized to that of an isotopically labeled analog.^{19 ,21 (link)} For other PFASs, the analyte response was normalized to that of an isotopically labeled PFAS with an LC retention time similar to that of the analyte (Table S1). Details of the PFAS quantitation and quality assurance/quality control (QA/QC) are provided in the Supporting Information (Texts S2–S3).

Zhang C., McElroy A.C., Liberatore H.K., Alexander N.L, & Knappe D.R. (2021). Stability of Per- and Polyfluoroalkyl Substances in Solvents Relevant to Environmental and Toxicological Analysis. Environmental science & technology, 56(10), 6103-6112.

+ Open protocol

+ Expand

Untargeted Metabolite Profiling by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

For untargeted metabolite production assays, 200 μl of yeast culture from 96-well plates was flash-frozen, lyophilized overnight, and dissolved in 100 μl of 75% methanol (with 25% water) with 0.1% formic acid. The sample was analyzed by the Agilent 1260 Infinity Binary HPLC paired with an Agilent 6545 Quadrupole Time-of-Flight LC-MS, with a reversed-phase column (Agilent EclipsePlus C18, 2.1 × 50 mm, 1.8 μm), water with 0.1% formic acid as solvent A, and acetonitrile with 0.1% formic acid as solvent B, at a constant flow rate of 0.6 ml/min and an injection volume of 1 μl. The following gradient was used for compound separation: 0 to 0.40 min, 5% B; 0.40 to 8.40 min, 5 to 95% B; 8.40 to 10.40 min, 95% B; 10.40 to 10.41 min, 95 to 5% B; 10.41 to 12.00 min, 5% B. The liquid chromatogram eluent was directed to the MS for 1 to 12 min with ESI source in positive mode, gas temperature at 250°C, gas flow rate at 12 liters/min, nebulizer pressure at 10 psig, Vcap at 3500 V, fragmentor at 100 V, skimmer at 50 V, octupole 1 RF Vpp at 750 V, and acquisition scan rate at 2.50 spectra/s.

Kong D., Li S, & Smolke C.D. (2020). Discovery of a previously unknown biosynthetic capacity of naringenin chalcone synthase by heterologous expression of a tomato gene cluster in yeast. Science Advances, 6(44), eabd1143.

+ Open protocol

+ Expand

Comprehensive Lipid Profiling Using QTOF-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were analyzed using an Agilent 1290 Infinity II UHPLC system coupled to an Agilent 6545 quadrupole time-of-flight (QTOF) mass spectrometer. The Agilent 1290 Infinity II Multisampler system, equipped with a multiwash option, was used to uptake 1 µl for the positive ionization mode and 2 µl for the negative ionization mode of the extracted samples. The method is described in detail in Supplementary Data 1 and in our previous publications (28 (link)). Data were processed using MassHunter Qualitative software v B.10.00 (Agilent Technologies Inc.) and MassHunter Profinder software v 10.0.2. MS/MS data sets were processed using MassHunter Lipid Annotator (Agilent Technologies Inc., Santa Clara, CA, USA) and MS-DIAL v.4 (RIKEN Center of Sustainable Resource Science, Yokahoma City, Kanagawa, Japan). To complete the lipid series, a tentative identification of lipid features was carried out based on Full Scan (MS1) data, retention time mapping (RT mapping), and the literature, using the online tool CEU Mass Mediator (CMM) (29 (link)) and the software MassHunter Qualitative v 10.0 (Agilent Technologies Inc). A final in-house library of glycerophospholipids (GPs) and sphingomyelins (SMs) was generated and used for the identification of lipid species.

Piédrola I., Martínez S., Gradillas A., Villaseñor A., Alonso-Herranz V., Sánchez-Vera I., Escudero E., Martín-Antoniano I.A., Varona J.F., Ruiz A., Castellano J.M., Muñoz Ú, & Sádaba M.C. (2023). Deficiency in the production of antibodies to lipids correlates with increased lipid metabolism in severe COVID-19 patients. Frontiers in Immunology, 14, 1188786.

+ Open protocol

+ Expand

Untargeted Metabolite Profiling of Plant Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

The metabolite profiling of roots and shoots was performed using an Agilent Infinity 1290 UHPLC system coupled to Agilent 6545 quadrupole time of flight (QTOF) mass spectrometer equipped with an Agilent dual-jet stream electrospray ion source. The whole system was controlled by Masshunter software. An HSS T3 (C18) 1.8 μ m, 2.1 mm × 150 mm was used for chromatographic separation of non-targeted compounds with a flow rate of 0.5 mL/min and a sample volume of 10 μ L was injected for each run. The temperature of the column oven was set at 40 °C. The mobile phases were (A): 100% LC-MS grade water with 0.02 M formic and (B): acetonitrile with 0.02 M formic acid. The gradient was as follows: 0 min, 0% B; 1 min, 0% B; 3 min, 10% B; 10 min, 70% B; 15 min, 100% B; 17.5 min, 100% B; 18 min, 0% B; 21 min, 0% B. Spectra were acquired in full scan MS1 and data-dependent MS2. Data were collected in ESI(±) modes with a mass range of m/z 75-1500 Da. QTOF-MS parameters were set as follows: Fragmentor voltage at 120 V, capillary voltage at 4000 V, skimmer voltage at 65 V, collision energy at 30 eV, drying gas temperature at 325 °C (8 L/min), nebulising gas pressure at 40 psi, sheath gas temperature and flow at 300 °C and 12 L/min, respectively.

Hazrati H., Fomsgaard I.S., Ding L., & Kudsk P. (2021). Mass spectrometry-based metabolomics unravel the extensive transfer of bioactive compounds between rye and neighboring plants.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!