All tissue samples were obtained from patients treated at The First Affiliated Hospital of Zhengzhou University. Each patient signed an informed consent form, and this study was approved by the First Affiliated Hospital of Zhengzhou University Institutional Review Board. Tissues samples were collected from OC patients with unilateral ovarian invasion and normal contralateral ovary, including 17 cases of high-grade serous carcinoma, 2 cases of mucinous cystadenoma and 1 case of mucinous carcinoma. Human OC cell lines (A2780, SKOV3, OVCAR3) and a normal ovary cell line (IOSE80) were purchased from the Cell Bank of Chinese Academy of Sciences (Shanghai, China). All cell lines were authenticated using the short tandem repeat (STR) profiling test. Then, IOSE80, A2780, OVCAR3 cells were cultured in RPMI-1640 Medium (Biological Industries, USA) supplemented with 10% fetal bovine serum (FBS, Biological Industries, USA), 100 units/ml penicillin, and 100 μg/ml streptomycin (Biological Industries, USA). SKOV3 cell was maintained in McCoy’s 5A Medium (Biological Industries, USA) supplemented with 10% FBS, 100 U/ml penicillin, and 100 μg/ml streptomycin.
Ovcar3
Manufactured by Sartorius
Sourced in Israel
The OVCAR3 is a laboratory equipment product. It is a cell line derived from a human ovarian adenocarcinoma. This product is intended for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Sartorius (3 mentions)
A2780, by Sartorius (2 mentions)
Mccoy s 5a medium, by Sartorius (2 mentions)
Skov3, by Sartorius (2 mentions)
Ovcar3, by American Type Culture Collection (2 mentions)
Dulbecco modified eagle medium (dmem), by Sartorius (2 mentions)
Penicillin, by Sartorius (1 mentions)
Streptomycin, by Sartorius (1 mentions)
Iose80, by Sartorius (1 mentions)
A2780, by American Type Culture Collection (1 mentions)
Skov3, by American Type Culture Collection (1 mentions)
Penicillin streptomycin, by Beyotime (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
L glutamine, by Sartorius (1 mentions)
Non essential amino acids (neaa), by Sartorius (1 mentions)
Vitamin solution, by Sartorius (1 mentions)
Ovcar8, by Sartorius (1 mentions)
Sodium pyruvate, by Sartorius (1 mentions)
Ex cell advanced cho fed batch medium, by Merck Group (1 mentions)
Fetal calf serum (fcs), by Merck Group (1 mentions)
4 protocols using ovcar3
1
Ovarian Cancer Tissue Samples and Cell Lines
2
Culturing Ovarian Cancer Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The SKOV3, A2780 and OVCAR-3 human epithelial ovarian cancer cell lines were obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA). Their TP53 status were null (SKOV3), wild-type (A2780) and mutant (OVCAR-3). The SKOV3 cells were maintained in McCoy's 5A medium (Biological Industries, Beit Haemek, Israel), A2780 cells and OVCAR-3 cells were cultured in RPMI 1640 medium (Biological Industries), both all supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin. Cells were maintained at 37°C with an atmosphere of 5% CO2, following standard protocols. The medium was replaced two-to-three times every week.
3
Ovarian Cancer Cell Culture Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The human ovarian cancer cell lines SKOV3, A2780 and OVCAR3 were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). The SKOV3 and OVCAR3 cells were cultured in RPMI-1640 medium (Biological Industries, Kibbutz Beit-Haemek, Israel), and A2780 cells were cultured with DMEM (Biological Industries, Kibbutz Beit-Haemek, Israel). All the cells were supplemented with 10% fetal bovine serum (FBS, Biological Industries, Kibbutz Beit-Haemek, Israel), 1% penicillin/streptomycin (Beyotime, Jiangsu, China) and incubated at 37 °C with 5% CO2.
4
Ovarian Cancer Cell Line Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The OVCAR3 and OVCAR8 OC cell lines, both widely regarded to originate from HGSC, were obtained from the American Type Culture Collection (ATCC) and cultured according to the manufacturer’s instructions. OVCAR3 cells were cultured in DMEM, OVCAR8 in RPMI (Biological Industries, Beit-Haemek, Israel). The medium was supplemented with 1% L-glutamine, 1% sodium pyruvate, 1% vitamin solution, 1% non-essential amino acids (Biological Industries) and 10% fetal calf serum (Sigma-Aldrich, St. Louis, MO, USA). Cells were grown in a humidified atmosphere of 95% air and 5% CO2. In cells from which exosomes were extracted, the medium used was EXCELL® Advanced™ CHO Fed-batch Medium (Sigma-Aldrich). OVCAR3 and OVCAR8 OC spheroids were formed by constant shaking of 400,000 cells per well in 6-well plates for 48 h on a vertical shaker.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!