Gsk1904529a

Manufactured by Selleck Chemicals

Sourced in United States

GSK1904529A is a lab equipment product used for chemical and biological research applications. It is a high-performance liquid chromatography (HPLC) system, designed to separate, identify, and quantify the components of a mixture. The core function of GSK1904529A is to provide accurate and reliable analytical results for researchers and scientists.

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Lab products found in correlation

5 protocols using gsk1904529a

Functional Regulation of miRNA Targets

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Hsa-miR-483-5p mimic, YM00473215-ADA, mimic control, YM00479902-ADA, inhibitors hsa-miR-675-5p, 427419-00, hsa-miR-675-3p, 427420-00, hsa-miR-483-5p, 427155-00, hsa-miR-483-3p, 427154-00, hsa-miR-204-5p, 426934-00, hsa-miR-135-5p, 426790-00, hsa-miR-10a-5p, 426651-00, hsa-miR-9-5p, 427460-00, hsa-miR-9-3p, 427461-00, Control B, 199021-00 are from Qiagen, Germantown, MD, USA. Ceritinib, S7083, linsitinib, S1091, entrectinib, S7998, GSK1904529A, S1093, picropodophyllin, S7668 were obtained from Selleck Chemicals, Houston, TX, USA; GSK1838705A, SML0995, was purchased from Millipore-Sigma, Burlington, MA, USA. The primers used for miRNA and mRNA quantification were miR-99b-5p, 4,427,975, miR-483-5p, 4,427,975, miR-675-5p, 4,427,975, IGF-2, 4,331,182, GAPDH, 4,331,182, ThermoFisher Scientific, Waltham, MA, USA.

Uhlmann E.J., Mackel C.E., Deforzh E., Rabinovsky R., Brastianos P.K., Varma H., Vega R.A, & Krichevsky A.M. (2023). Inhibition of the epigenetically activated miR-483-5p/IGF-2 pathway results in rapid loss of meningioma tumor cell viability. Journal of Neuro-Oncology, 162(1), 109-118.

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Adipogenic Differentiation of hMSCs

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hMSCs were seeded into four-well plates and exposed to adipogenic induction media composed of DMEM, 10% fetal bovine serum (FBS), 10% horse serum (Gibco, U.S.A.), 100 µM dexamethasone (Sigma, U.K.), 1 µM Rosiglitazone (BRL) (Novo Nordisk Bagsvaerd, Denmark), 3 µg/ml insulin (Sigma, U.K.), 450 µM isobutylmethylxanthine (IBMX) (Sigma, U.K.), and 1% penicillin-streptomycin (Sigma, U.K.) supplemented with FAK inhibitors (PF-573228 and PF-562271) or IGF-1R/InsR inhibitors (NVP-AEW541 and GSK1904529A), which were purchased from Selleckchem Inc. (Selleckchem Inc., Houston, TX, U.S.A.). Inhibitors were used at 5 µM throughout all experiments. Adipocyte induction medium (AIM) was changed every 2 days and for 7 days. Previous published work from our group indicated day 7 as a time point on which adipogenic markers were up-regulated significantly in an enriched population of 70–80% of adipogenic populations [8 (link)].

Ali D., Abuelreich S., Alkeraishan N., Shwish N.B., Hamam R., Kassem M., Alfayez M., Aldahmash A, & Alajez N.M. (2018). Multiple intracellular signaling pathways orchestrate adipocytic differentiation of human bone marrow stromal stem cells. Bioscience Reports, 38(1), BSR20171252.

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DHCR24 knockdown and drug sensitivity

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For the drug sensitivity assay, KLE and HEC-1B cells were transfected with DHCR24 siRNA and followed by treatment with MPA at a concentration of 10 μM for 48 hours. Cell viability was measured using a CCK-8 assay (Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions. Cell survival was also measured after treatment with MPA (10 μM), insulin (100 nmol/l), GSK1904529A (60 nM), and Stattic (10 μM). MPA, GSK1904529A and Stattic were obtained from Selleck.

Dai M., Zhu X.L., Liu F., Xu Q.Y., Ge Q.L., Jiang S.H., Yang X.M., Li J., Wang Y.H., Wu Q.K., Ai Z.H., Teng Y.C, & Zhang Z.G. (2017). Cholesterol Synthetase DHCR24 Induced by Insulin Aggravates Cancer Invasion and Progesterone Resistance in Endometrial Carcinoma. Scientific Reports, 7, 41404.

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Glioma Spheroid Viability Assay

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The following inhibitors were used: dasatinib (Selleck, S1021), IR/IGFR1 inhibitor GSK1904529A (Selleck S1093), Notch/Gamma secretase inhibitor Compound E (Millipore 565790), and MEK/ERK1/2 pathway inhibitor PD98059 (Selleck S1177). Accutase-dissociated gliomaspheres were counted and plated at 1000–5000 cells/well of a 96-well plate in 125 μL Neurobasal with growth factors with either DMSO or dasatinib and/or other targeted inhibitors in at least triplicate. A set of cells of each type were analyzed for total ATP content at day 0 for normalization. Cells were permitted to grow for 7 days before they were analyzed per manufacturer’s protocol by Cell Titer Glo (Promega) alongside ATP standards for end point luminescence on a Synergy HTX Platereader (BioTek). At least 4 separately treated replicates were quantified for each condition.

Eyler C.E., Matsunaga H., Hovestadt V., Vantine S.J., van Galen P, & Bernstein B.E. (2020). Single-cell lineage analysis reveals genetic and epigenetic interplay in glioblastoma drug resistance. Genome Biology, 21, 174.

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D. latiflorus Leaf Extract Effects

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D. latiflorus leaves were purchased from Maoshennongye Co., Ltd. (Shandong, China). HepG2 cell line was obtained from the National Infrastructure of Cell Line Resource, Peking Union Medical College (Beijing, China). Fetal bovine serum (FBS) Uruguay was purchased from Biowest (Nuaillé, France). Dulbecco's modified Eagle's medium (DMEM) with high glucose, liquid and other cell culture reagents were purchased from HyClone (Logan, UT, USA). AKT antibody, phospho-AKT (Ser473) (D9E) XP rabbit mAb, FOXO1 (C29H4) rabbit mAb, phospho-FOXO1 (Ser256) antibody, GSK-3β (D5C5Z) XP rabbit mAb, phospho-GSK-3β (Ser9) (D85E12) XP rabbit mAb, and GAPDH (14C10) rabbit mAb were purchased from Cell Signaling Technology (Beverly, MA, USA). The insulin growth factor 1 receptor (IGF1R) inhibitor GSK1904529A was purchased from Selleck Chemicals (Houston, TX, USA).

Luo K., Huang W., Qiao L., Zhang X., Yan D., Ning Z., Ma C., Dang H., Wang D., Guo H., Xie L, & Cheng J. (2021). Dendrocalamus latiflorus and its component rutin exhibit glucose-lowering activities by inhibiting hepatic glucose production via AKT activation. Acta Pharmaceutica Sinica. B, 12(5), 2239-2251.

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