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293ft cells r70007

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293FT cells are a human embryonic kidney cell line that is commonly used for the production of lentiviral vectors. These cells are engineered to stably express the large T antigen of SV40, which enhances their ability to support high-level expression of recombinant proteins. The 293FT cells are provided frozen and can be cultured using standard cell culture techniques.

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Lab products found in correlation

Cc 2540, by Lonza (2 mentions) Hek293t crl 3216, by American Type Culture Collection (2 mentions) Begm bullet kit, by Lonza (2 mentions) C6 36 cells, by American Type Culture Collection (2 mentions) Hela h1, by American Type Culture Collection (2 mentions) Rd ccl 136, by American Type Culture Collection (2 mentions) Non essential amino acids solution, by Thermo Fisher Scientific (1 mentions) Pei max reagent, by Cosmo Bio (1 mentions)

3 protocols using 293ft cells r70007

1

Cell Culture Protocols for Diverse Cell Lines

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C6/36 cells (CRL-1660), H1-Hela (CRL-1958), HEK293T (CRL-3216), and RD (CCL-136) cells were obtained from American Type Culture Collection (ATCC). 293FT cells (R70007) were purchased from Thermo Fisher. All transformed cell lines were cultured in DMEM/high glucose supplemented with 1× penicillin-streptomycin, 1× L-glutamine, and 10% heat-inactivated fetal bovine serum. Human primary normal bronchial epithelial cells (NHBE, Lonza CC-2540) were purchased from Lonza and cultured according to the manufacturer’s recommendation using BEGM BulletKit (Lonza CC-3171 & CC-4175).
Diep J., Ooi Y.S., Wilkinson A.W., Peters C.E., Foy E., Johnson J.R., Zengel J., Ding S., Weng K.F., Laufman O., Jang G., Xu J., Young T., Verschueren E., Kobluk K.J., Elias J.E., Sarnow P., Greenberg H.B., Hüttenhain R., Nagamine C.M., Andino R., Krogan N.J., Gozani O, & Carette J.E. (2019). Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3. Nature microbiology, 4(12), 2523-2537.
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2

Cell Culture Protocols for Diverse Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
C6/36 cells (CRL-1660), H1-Hela (CRL-1958), HEK293T (CRL-3216), and RD (CCL-136) cells were obtained from American Type Culture Collection (ATCC). 293FT cells (R70007) were purchased from Thermo Fisher. All transformed cell lines were cultured in DMEM/high glucose supplemented with 1× penicillin-streptomycin, 1× L-glutamine, and 10% heat-inactivated fetal bovine serum. Human primary normal bronchial epithelial cells (NHBE, Lonza CC-2540) were purchased from Lonza and cultured according to the manufacturer’s recommendation using BEGM BulletKit (Lonza CC-3171 & CC-4175).
Diep J., Ooi Y.S., Wilkinson A.W., Peters C.E., Foy E., Johnson J.R., Zengel J., Ding S., Weng K.F., Laufman O., Jang G., Xu J., Young T., Verschueren E., Kobluk K.J., Elias J.E., Sarnow P., Greenberg H.B., Hüttenhain R., Nagamine C.M., Andino R., Krogan N.J., Gozani O, & Carette J.E. (2019). Enterovirus Pathogenesis Requires the Host Methyltransferase SETD3. Nature microbiology, 4(12), 2523-2537.
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3

Cell Culture and Transfection Protocol

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HEK293T (RCB2202) cells were provided by the RIKEN BRC through the National Bio-Resource Project of MEXT, Japan. They were grown in Dulbecco’s modified Eagle’s medium (DMEM) with 10% fetal bovine serum (FBS) and penicillin/streptomycin (PS) (100 U/mL). 293FT cells (R700-07) were from Thermo Fisher and were grown in DMEM with 10% FBS, 1x Non-Essential Amino Acids Solution (Thermo Fisher 11140-050), and PS (100 U/mL). Plasmid transfection was performed using the PEI-MAX reagent (Cosmo Bio, Koto, Tokyo, Japan). Used cell lines in this study are listed in Supplementary Table 3.
Narita M., Denk T., Matsuo Y., Sugiyama T., Kikuguchi C., Ito S., Sato N., Suzuki T., Hashimoto S., Machová I., Tesina P., Beckmann R, & Inada T. (2022). A distinct mammalian disome collision interface harbors K63-linked polyubiquitination of uS10 to trigger hRQT-mediated subunit dissociation. Nature Communications, 13, 6411.
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