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Anti c myc sc 40

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Anti-c-Myc (sc-40) is a mouse monoclonal antibody that recognizes the c-Myc protein. The c-Myc protein is a transcription factor that plays a role in cell proliferation, growth, and apoptosis. The antibody can be used for the detection of c-Myc in various applications, such as Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Anti gapdh, by Cell Signaling Technology (2 mentions) Anti cleaved caspase 3, by Cell Signaling Technology (2 mentions) Anti flag, by Merck Group (1 mentions) Anti flag m2 affinity gel, by Merck Group (1 mentions) Anti survivin ab469, by Abcam (1 mentions) Anti β catenin sc 7963, by Santa Cruz Biotechnology (1 mentions) Anti gapdh gtx627408, by GeneTex (1 mentions) Las 3000 system, by Fujifilm (1 mentions) Pvdf membrane, by Merck Group (1 mentions) Anti erk, by Cell Signaling Technology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti akt, by Cell Signaling Technology (1 mentions) Anti gsk3β, by Cell Signaling Technology (1 mentions) Anti p stat3, by Cell Signaling Technology (1 mentions) Anti p akt, by Cell Signaling Technology (1 mentions) Anti p β catenin, by Cell Signaling Technology (1 mentions) Anti stat3 sc 8019, by Santa Cruz Biotechnology (1 mentions) Anti stat5, by Cell Signaling Technology (1 mentions) Anti β catenin, by Cell Signaling Technology (1 mentions) Anti p stat5, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

C-Myc (386 citations) Anti-Myc (207 citations) Anti-c-Myc (180 citations) Sc-40 (89 citations) Anti-Myc (sc-40, (32 citations) C-Myc (sc-40) (27 citations) MYC (sc-40, (22 citations) Anti-c-Myc (9E10; sc-40; (8 citations) Mouse anti c-MYC[9E10] (sc-40)( (3 citations) Mouse anti-c-Myc (sc-40; (3 citations)

17 protocols using anti c myc sc 40

1

Immunoprecipitation and Western Blot Assays

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Immunoprecipitation and western blot assays were done as previously described35 using either anti-FLAG M2 Affinity Gel (Sigma), anti-c-Myc (sc-40, Santa Cruz, Dallas, TX, USA) or anti-Gal4DBD (sc-577, Santa Cruz) antibodies. Western blot analyses were performed using anti-FLAG (Sigma) or anti-c-Myc (sc-40, Santa Cruz) antibodies.
Jalaguier S., Teyssier C., Nait Achour T., Lucas A., Bonnet S., Rodriguez C., Elarouci N., Lapierre M, & Cavaillès V. (2017). Complex regulation of LCoR signaling in breast cancer cells. Oncogene, 36(33), 4790-4801.
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2

Immunoblot Analysis of Apoptosis Regulators

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CRC cells (4 × 105) were treated with Obatoclax (0, 100, 200 μM) for 24 h, or treated with Obatoclax for 22 h and then combined with MG132 (20 μM) for an additional 2 h. After drug treatment, cells were lysed, subjected to SDS-PAGE, blot transfer, incubation with primary and secondary antibodies, exposure to enhanced chemiluminescence (ECL) reagent, and final visualization of the antigen-of-interest using the LAS3000 system (Fujifilm; Tokyo, JPN) as stated previously [45 (link),46 (link)]. Densitometry analysis of immunoblot signals was accomplished by ImageJ software (National Institute of Health, Bethesda, MD, USA). Anti-survivin (#ab469) and anti-TCF4 (#ab76151) antibodies were bought from Abcam (Cambridge, GBR). Anti-β-catenin (sc-7963) and anti-c-MYC (sc-40) were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-LEF1 (GTX129186) and anti-GAPDH (GTX627408) were obtained from GeneTex (Hsinchu, TWN), and anti-β-tubulin (#T2200) was bought from Sigma-Aldrich (St. Louis, MO, USA). Primary antibodies against cyclin D1 (#2926), HA-tag (#3724), and cleaved forms of caspases-3 (#9664), -8 (#9496), -9 (#9501), and PARP (#9541) were all purchased from Cell signaling Technology (Boston, MA, USA).
Or C.H., Huang C.W., Chang C.C., Lai Y.C., Chen Y.J, & Chang C.C. (2020). Obatoclax, a Pan-BCL-2 Inhibitor, Downregulates Survivin to Induce Apoptosis in Human Colorectal Carcinoma Cells Via Suppressing WNT/β-catenin Signaling. International Journal of Molecular Sciences, 21(5), 1773.
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3

Rac1 Activation and Signaling Pathway Analysis

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Active Rac1 pull-down assay and Western blot analysis were performed as previously described [4 (link)]. MCF7 Cells incubated with polypeptides at 1.0 μM were lysed in a NP-40 lysis buffer (0.15 M NaCl, 1% NP-40, and 0.05 M Tris-HCl, pH 8.0) with a mixture of protease inhibitors (0.25 mM phenylmethylsulfonyl fluoride, 10 mg/mL aprotinin and leupeptin, and 1 mM dithiothreitol). Approximately 20 μg of total protein was separated via 10% SDS-PAGE and transferred to a PVDF membrane (Millipore, MA, USA). The primary antibodies purchased from Cell Signaling Technology were as follows: anti-p-Akt (Thr308, #13038), anti-p-Akt (Ser473, #4060), anti-Akt (#4691), anti-p-ERK (Thr202/Tyr204, #4094), anti-ERK(#4695), anti-p-STAT5 (Tyr694, #4322), anti-p-STAT3 (Tyr705, #4113), anti-STAT5(#94205), anti-p-GSK-3β (Ser9, #9323), anti-GSK-3β (#12456), anti-p-β-catenin (Ser33/37/Thr41, #9561), anti-p-β-catenin (Thr41/Ser45, #9561), anti-p-β-catenin (Ser552, #5651), anti-p-β-catenin (Ser675, #4176), anti-β-catenin (#8480), and anti-GAPDH (#5174). The primary antibodies purchased from Santa Cruz Biotechnology were anti-STAT3 (SC-8019) and anti-c-Myc (SC-40).
Zhang Z., Luo Z., Min W., Zhang L., Wu Y, & Hu X. (2017). An anti-cancer WxxxE-containing azurin polypeptide inhibits Rac1-dependent STAT3 and ERK/GSK-3β signaling in breast cancer cells. Oncotarget, 8(26), 43091-43103.
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4

Delineating the Ubiquitin-Proteasome Pathway

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Anti-Cdc20 (p55 CDC, sc-5296 and sc-13162), anti-Cdh1 (Fzr, sc-56312), anti-p27 (SC-527), anti-HA antibody (SC-805), anti-c-Myc (sc-40), and anti-p27 (SC-527) antibodies were purchased from Santa Cruz. Anti-SPOP antibody (16750-1-AP) was purchased from Proteintech. Mouse monoclonal anti-Myc-Tag (2276), rabbit polyclonal anti-Myc-Tag antibody (2278), anti-Cullin 3 (2759), and anti-GST (2625) antibodies were purchased from Cell Signaling. Polyclonal anti-Flag antibody (F-2425), monoclonal anti-Flag antibody (F-3165, clone M2), anti-vinculin antibody (V-4505), anti-tubulin antibody (T5168), peroxidase-conjugated anti-mouse secondary antibody (A-4416), peroxidase-conjugated anti-rabbit secondary antibody (A-4914), anti-HA agarose beads (A-2095) and anti-Flag agarose beads (A-2220) were purchased from Sigma. All antibodies were used in 1:1000 dilutions in 5% non-fat milk for western blot. MLN4924 was a kind gift from Dr. William Kaelin (Dana-Farber cancer institute). MG132 (BML-PI102-0005) was purchased from Enzo life science. Apcin (I-444) was purchased from BostonBiochem.
Wu F., Dai X., Gan W., Wan L., Li M., Mitsiades N., Wei W., Ding Q, & Zhang J. (2016). Prostate cancer-associated mutation in SPOP impairs its ability to target Cdc20 for poly-ubiquitination and degradation. Cancer letters, 385, 207-214.
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5

Comprehensive Purine Biosynthesis Pathway Examination

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Primary antibodies: anti-IMPDH1 (WH0003614M1, dilution 1:200) antibody (Sigma-Aldrich); anti-IMPDH2 (ab131158, dilution 1:400) antibodies (Abcam); anti-GFP (11814460001, dilution 1:1000) antibody (Roche); anti-c-Myc (sc-789, dilution 1:400), anti-c-Myc (sc-40, dilution 1:400), anti-nm23-H1 (sc-465, dilution 1:400) antibodies (Santa Cruz Biotechnology); and anti-GAPDH (#5174, dilution 1:400) antibody (Cell Signaling Technology). Secondary antibodies: goat anti-mouse IgG Alexa 488 (#A-11029), goat anti-rabbit IgG Alexa 568 (#A-11011), and goat anti-rabbit IgG Alexa 488 (#A-11034) antibodies (Invitrogen); Alexa Fluor 546 phalloidin (#A-12380, dilution 1:200) probe (Invitrogen); and DAPI. Plasmids: PRPS1-Myc-DDK (#RC200698), GUK1-Myc-DDK (#RC202510), Atic-Myc-DDK (#MR219425), ADK-Myc-DDK (#RC200628), APRT-Myc-DDK (#RC216874), HPRT1-Myc-DDK (#RC200462), Pnp-Myc-DDK (#MR203940), Ampd1-Myc-DDK (#MR220907), Nt5c1a-Myc-DDK (#MR217369), GAPDH-Myc-DDK (#MR204932) were purchased from OriGene; FGAMS-EGFP (#99107), PAICS-EGFP (#99108), GFP-ATIC (#99110), PPAT-EGFP (#99105), ADSL-EGFP (#99109), ADSS-V5 (#98316) were purchased from Addgene. Rat GMPS (rGMPS) and mCherry were amplified using standard PCR procedures and combined into the pTRIPZ vector using the Gibson assembly cloning kit (New England Biolabs #E5510S).
Wolfe K., Kofuji S., Yoshino H., Sasaki M., Okumura K, & Sasaki A.T. (2019). Dynamic Compartmentalization of Purine Nucleotide Metabolic Enzymes at Leading Edge in Highly Motile Renal Cell Carcinoma. Biochemical and biophysical research communications, 516(1), 50-56.
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6

Protein Expression Analysis by Western Blotting

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Standard western blotting procedure was used. Primary antibodies (all antibodies diluted at 1/1000, except for β-Actin at 1/20,000): anti-FLAG (F1804; Sigma), anti-β-Actin (GT5512; GeneTex), anti-RPS24 (A303-842A; Bethyl), anti-RPS12 (ab175219; Abcam), anti-RPL5 (ab137617; Abcam), anti-p53 (sc-126; Santa Cruz), and anti-c-Myc (sc-40, Santa Cruz). Secondary antibodies diluted at 1/5000 were HRP-conjugated anti-rabbit and anti-mouse (Promega). Densitometry was performed using Image Lab (BioRad).
Brumwell A., Fell L., Obress L, & Uniacke J. (2020). Hypoxia influences polysome distribution of human ribosomal protein S12 and alternative splicing of ribosomal protein mRNAs. RNA, 26(3), 361-371.
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7

Immunohistochemical and Immunofluorescent Analysis of Rac1, Stem Cell, and Cell Cycle Markers

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Immunohistochemistry and immunofluorescence were performed as previously described (14 ). Antibodies used for immunohistochemistry were anti-Rac1 (BD 610650, BD Transduction Laboratories™), anti-phospho-Rac1 (#44-214G, Life technology), anti-phospho-JNK (#4668, Cell Signaling), anti-Sox2 (#14962, Cell Signaling), anti-PCNA (sc-56, Santa Cruz Biotechnology), and anti-cleaved caspase-3 (#9661, Cell Signaling). To quantify immunohistochemical staining, images were digitally scanned with Panoramic Flash 250 (3DHistech, Budapest, Hungary) using 20×/0.8NA objective. Stained tissues were counted in five microscopic fields. The analysis was performed in Imaris 7.6 (Bitplane). Phospho-Rac1 stain was predominantly cytosol. Phospho-Rac1 scores (0–300) were calculated by multiplying the staining intensity (0, 1, 2, or 3) by the staining extent (0%– 100%).
For immunofluorescence, antibodies used were anti-Rac1 (BD 610650, BD Transduction Laboratories™), anti-CD44 (#5640, Cell Signaling), anti-Sox2 (#3579, Cell Signaling), anti-Oct-4 (#83932, Cell Signaling), anti-Nanog (#8822, Cell Signaling), anti-c-Myc (sc-40, Santa Cruz Biotechnology), anti-N-cadherin (BD 610920, BD Transduction Laboratories™), and anti-Slug (#9585, Cell Signaling).
Yoon C., Cho S.J., Chang K.K., Park D.J., Ryeom S.W, & Yoon S.S. (2017). Role of Rac1 pathway in epithelial-to-mesenchymal transition and cancer stem-like cell phenotypes in gastric adenocarcinoma. Molecular cancer research : MCR, 15(8), 1106-1116.
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8

Hepatocellular Carcinoma Cell Line Cultivation

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The human HCC cell lines HepG2, BEL-7402, SK-HEP1, SMMC-7721, and normal hepatic cell line LO2 were from China Center for Type Culture Collection and cultured in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum (FBS), penicillin (100 U/ml) and streptomycin (100 ng/ml) in a humidified incubator at 37°C with 5% CO2 atmosphere. U0126, LY294002, and Gefitinib were from ApexBio. Anti-TF (ab17375) and Anti-Ki-67 (2724-1) were from Abcam. Anti-pAKT (4060), Anti-AKT (4691), Anti-pERK (4370), and Anti-ERK (4695) antibodies were from Cell Signaling Technologies. Anti-EGFR (SC-03) and Anti-c-Myc (SC-40) antibodies were from Santa Cruz Biotechnology. Anti-β-actin (LK9001T) and Anti-GAPDH (LK9002T) antibodies were from Tianjin Sungene Biotech.
Huang S.Z., Wei M.N., Huang J.R., Zhang Z.J., Zhang W.J., Jiang Q.W., Yang Y., Wang H.Y., Jin H.L., Wang K., Xing Z.H., Yuan M.L., Li Y., He X.S., Shi Z, & Zhou Q. (2019). Targeting TF-AKT/ERK-EGFR Pathway Suppresses the Growth of Hepatocellular Carcinoma. Frontiers in Oncology, 9, 150.
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9

Immunoblotting and Immunoprecipitation Reagents

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Reagents and their suppliers were as follows: Chelex 100 resin from BioRad (142-2832; Mississauga, ON, Canada), cholera toxin (100) from List Biological (Campbell, CA, USA), leptomycin B (ALX-380-100) from Enzo Life Sciences (Plymouth, PA), PEI (25 kDa linear, Cat. No. 23966) from Polysciences (Warrington, PA). All other reagents were purchased from Sigma (St. Louis, MO, USA). The antibodies used were: anti-V5 (R960-25; Invitrogen, Carlsbad, CA), anti-HA (Y-11) from Santa Cruz (Santa Cruz, CA), anti-glyceraldehyde 3-phosphodehydrogenase (GAPDH; ADI CSA 335) from Enzo Life Sciences (Farmingdale, NY), anti-γ-tubulin (T6557) from Sigma (St. Louis, MO); anti-Cdh1 (DH01-DCS-266, ab3242, Abcam, Cambridge, UK), and anti-c-myc (SC-40, Santa Cruz). Horseradish peroxidase-conjugated goat anti-mouse (115–035-146) and anti-rabbit (111-035-144) antibodies were from Jackson Immuno Research Laboratories (West Grove, PA). Alexa Fluor®-conjugated goat anti-rabbit and goat anti-mouse IgG were from Molecular Probes/Thermo Fisher Scientific (Eugene, OR). λ phosphatase (PO753S) was from New England Biolabs (Ipswich, MI).
Singh R.K, & Dagnino L. (2016). CDH1 regulates E2F1 degradation in response to differentiation signals in keratinocytes. Oncotarget, 8(3), 4977-4993.
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10

Antibody Validation for Western Blotting and IHC

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The antibodies used in this study were as follows: anti-USP28 (17707-1-AP, 1:1000 WB, 1:200 IHC, Proteintech); anti-MYCN (51705S, 1:1000 WB, 1:200 IHC, Cell Signaling Technology); anti-MYCN (sc-53993, 1:200 WB, Santa Cruz Biotechnology); anti-c-MYC (sc-40, 1:200 WB, Santa Cruz Biotechnology); anti-CyclinD1 (2922S, 1:1000 WB, Cell Signaling); anti-PARP (9542P, 1:1000 WB, Cell Signaling); anti-cleaved PARP (9664S, 1:1000 WB, Cell Signaling); anti-Flag (F3165 1:5000 WB, Sigma); anti-Flag (20543-1-AP, 1:2000 WB, Proteintech); anti-HA (901,503, 1:1000 WB, BioLegend); anti-Ub (SC-8017, 1:200 WB, Santa Cruz Biotechnology); anti-LSD1 (20813-1-AP, 1:1000 WB, Proteintech); anti-Ki67 (27309-1-AP, 1:1000 WB, Proteintech); anti-Actin (66009-1-Ig, 1:5000 WB, Proteintech); and anti-GAPDH (60004-1-Ig, 1:5000 WB, Proteintech); the secondary antibodies conjugated to horseradish peroxidase were used for Western blotting. The secondary antibodies of anti-mouse or anti-rabbit containing Alexa Fluor 488 or 594 were used for immunofluorescence staining (Jackson ImmunoResearch Laboratories).
Li J., Peng J., Wu L., Shen X., Zhen X., Zhang Y., Ma H., Xu Y., Xiong Q., Zhu Q, & Zhang P. (2023). The deubiquitinase USP28 maintains the expression of the transcription factor MYCN and is essential in neuroblastoma cells. The Journal of Biological Chemistry, 299(7), 104856.
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