For tumor growth, 1 × 105 transfected cells (FaDu and SCC-15) in 1:1 mixture of PBS and matrigel were injected subcutaneously into athymic, 4-6 week-old male nude mice (Charles River) [6 ]. All experiments were performed in accordance with the Animal Care and Use Committee guidelines. Each group consisted of 6 mice and each experiment was repeated two times. Mice were examined every day and mice showing signs of morbidity were immediately sacrificed according to University guidelines. After 4 weeks, mice were sacrificed and tumor weights were taken. Tumors were immediately snap frozen as well as processed as FFPE for histological and IHC analysis respectively. Data presented as mean ± S.E. of duplicate experiments.
Male nude mice
Manufactured by Charles River Laboratories
Sourced in China, United States
Male nude mice are a strain of laboratory mice that lack fur and have a deficiency in their immune system. These mice are commonly used in research to study various medical conditions and the effects of new treatments.
Automatically generated - may contain errors
Lab products found in correlation
Control 3xlaco inducible shrna, by Merck Group (1 mentions)
Water soluble coelenterazine, by Nanolight Technology (1 mentions)
Ivis lumina 2, by PerkinElmer (1 mentions)
Luciferin, by GoldBio (1 mentions)
Male wistar rats, by Charles River Laboratories (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Ripa buffer, by Beyotime (1 mentions)
Protease inhibitor, by Beyotime (1 mentions)
32 protocols using male nude mice
1
Subcutaneous Tumor Growth Assay
2
Inhibiting miR-200c-3p Suppresses Tumor Growth in Nude Mice
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Male nude mice (6–8 weeks old; Charles River Laboratories, Beijing, China) were acclimated in a specific-pathogen-free facility at the Experimental Animal Center of the Shaanxi Hospital of Traditional Chinese Medicine for at least 1 week. Stable K1 cells expressing the miR-200c-3p inhibitor or NC inhibitor were subcutaneously injected into the right flanks of the randomly selected nude mice at a dose of 1 × 106 cells per mouse. The tumor size was measured every 4 days. The tumor volume (V) was monitored once every 4 days and calculated using the following formula: V = ab2/2, where a is the long diameter, and b is the short diameter. The mice were sacrificed 18 days after the tumor cells were inoculated. Then, the tumors were isolated, weighed, imaged, and analyzed. All animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals (NRC 2011). The animal experimental procedures were approved by the Institutional Animal Care and Use Committee of Shaanxi Hospital of Traditional Chinese Medicine.
3
Mesothelioma Xenograft Model in Nude Mice
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Male nude mice (6–8 weeks old; weight 18–25 g) were obtained from Charles River. Mice were housed in the animal facility of the Regina Elena National Cancer Institute for 2 weeks before each experiment; animals had ad libitum water and food. The Ethics Committee of the Cancer Institute approved all the experimental protocols that were carried out in accordance with Italian regulations and with the Guide for the Care and Use of Laboratory Animals. A mouse xenograft model of mesothelioma was created as described previously [10 (link)]. MSTO cell suspensions (2.5 × 106) in 0, 2 ml of complete medium were injected subcutaneously into the flank of CD1 nude mice (n = 10/treatment group) and growth was measured twice weekly with calipers and calculated by the formula: 4/3 π (large diameter) × (small diameter)2. After the establishment of palpable lesions (average diameter > 5 mm), mice were assigned to one of the following treatment groups: 1) Control, 2) Exemestane (8.25 mg/Kg, intraperitoneal (i.p.) 5 days a week. Experimental groups were treated for 60 days. Mice were followed for tumour size and blood sampling from the tail were performed after 29 and 50 days from the beginning of treatment with exemestane.
4
Subcutaneous Tumor Growth in Nude Mice
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Male nude mice (4 weeks old) were purchased from Charles River and maintained in accordance with the Institutional Animal Care and Use Committee-approved protocol. 1 × 107cells were used for subcutaneous injection with Matrigel ECM (reconstituted basement membrane) to support tumor growth [37 (link)]. Each experimental group contained 10 mice. Tumors were measured with calipers every two days.
5
Interrogating CELF1 Function in Nude Mice
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Six week old male nude mice (Charles River Laboratories) were injected subcutaneously in the right flank with 5×106 UMSCC-74B cells stably transduced with control 3XlacO inducible shRNA and left flank of the same mouse with CELF1 3XlacO inducible shRNA (Sigma Aldrich) resuspended in 20% Matrigel. One-day post injection mice were treated with 21mM IPTG (Lab Scientific) and 5% glucose in the drinking water. Tumor volumes were measured twice weekly using digital calipers and mice were sacrificed when tumor volumes exceeded 3000mm3. Tumor volume formula = 0.5*(L*W2) W = shortest side L = longest side. (See supplemental materials and methods for statistical analysis).
6
Murine Model of Lung Metastasis
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The male nude mice aged 4–6 weeks were purchased from Charles River, China. A549 cells were stably transfected with BC or non‐coding vector with G418 resistance. Transfected cells (1 × 106) in 100 μl PBS were injected into the tail veins of BALB/C nude mice. The mice were sacrificed and dissected 4 weeks after injection. Death rates prior to sacrifice and metastatic foci in the lungs and pulmonary vessels were counted. All animal studies were conducted under approved guidelines of the Animal Care and Use Committee of Fudan University.
7
Subcutaneous Xenograft Tumor Model
8
Xenograft Model of Pancreatic Cancer
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Animal experiments were conducted under the approval of the Animal Research Ethics Committee of the First Affiliated Hospital of China Medical University. 4–6 weeks old male nude mice (Charles River, China) were selected, with 5 in each group. After transfection, 200 mL of PANC-1 cells (3 × 106) were injected subcutaneously into the left side of the back of each nude mouse. Tumor size was measured regularly and calculated using formula 0.52 × tumor length × tumor short diameter2. The animals were euthanized 30 d after injection to remove the tumor and measure its volume and weight.
9
Xenograft Tumor Growth Modulation
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106 MDA-MB-231 shNgBR stable cells were subcutaneously implanted to the flank region of 6–8 weeks male nude mice (Charles River Laboratories). When the average tumor volume reached about 200 mm3, mice were separated to two groups randomly and then fed with or without 2mg/ml doxycycline in 1% sucrose drinking water. The diameters of the tumors were measured every other day with a caliper. The tumor volume was calculated as Tumor volume=0.5 × length × width2. After three weeks of doxycycline administration, mice were sacrificed and tumors were isolated. Half of the tumor tissues were fixed in 4%PFA/PBS for histology analysis and another half were frozen in liquid nitrogen for gene expression analysis. For NIH-3T3 cell implantation, 106 NIH-3T3 control cells or highly expressing NgBR-HA cells (NIH-3T3-NgBR-HA) were subcutaneously implanted to the flank region of 6–8 weeks male nude mice. After 6–8 weeks, mice were sacrificed and tumor tissues were isolated as above described. Tumors from animals that died before the endpoint were excluded from the analysis. All of the animal experiments were approved by the Institutional Animal Care Use Committee of the Medical College of Wisconsin.
10
Subcutaneous Xenograft Tumor Model
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10 × 106 cells were suspended in 100 μL PBS, mixed with 100 μL matrigel, and injected subcutaneously into the flanks of 5-week-old male nude mice (Charles River Laboratories) on a normal chow diet. Tumor size measurements were taken over the course of 25–30 days. Tumor volumes were calculated using the ellipsoid formula (Pi/6)*L*W*H. Tumors were surgically removed to take final measurements and images. BrdU incorporation was analyzed by immunohistochemistry (detailed description in Extended Procedures). All procedures have been approved by the Institutional Animal Care and Use Committee of Yale University.
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