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Bioanalyzer system

Manufactured by Illumina

The Bioanalyzer system is a lab equipment product offered by Illumina. It is a microfluidics-based platform designed for the analysis and characterization of various biomolecules, including DNA, RNA, and proteins. The Bioanalyzer system provides automated, high-resolution electrophoretic separation and detection of these biomolecules within a miniaturized, integrated system.

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3 protocols using bioanalyzer system

1

DNA Sequencing Library Preparation

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Following DNA purifications, libraries were prepared using the TruSeq ChIP Library Prep Kit according to Illumina protocols, multiplexed library sizes were validated on an Agilent Bioanalyzer system and then sequenced (single-read) on an Illumina HiSeq 4000 sequencer.
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2

Transcriptome Analysis of Tumor Samples

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All RNAs used for quantitative gene expression analysis were extracted from fresh–frozen tissues. Quality of input RNA was assessed using the Agilent Bioanalyzer System and transcriptome analysis was performed using Illumina TruSeq strand-specific PolyA + libraries on an Illumina HiSeq4000 or NovaSeq device. Differential gene expression analysis was performed with the R2 Genomics Analysis and Visualization Platform (http://r2.amc.nl) using a reference cohort of embryonal tumors (n = 117), glial tumors (n = 126), and normal fetal and adult brain tissues (n = 36) that had been processed the same way. TPM values of the ET, PLAGL samples from our cohort are provided in the supplementary materials (Supplementary Table S8).
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3

RNA Sequencing Library Preparation

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Following RNA purification with trizol and the Qiagen RNAeasy Minelute kit (Cat.# 74204), libraries were prepared using the TruSeq RNA Library Prep Kit v2 according to Illumina protocols, multiplexed library sizes were validated on an Agilent Bioanalyzer system and then sequenced (single-read) on an Illumina HiSeq 4000 or Illumina NovaSeq 6000 sequencer.
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