The chemicals maltodextrin, colloidal silicon dioxide, (S)-(-)-6-Hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox®), Folin-Ciocalteu, gallic acid, aluminum trichloride (AlCl3), 2, 4, 6 - tripyridyl −1, 3, 5 - triazine (TPTZ), calcium chloride (CaCl2), α-amylase, pepsin, hydrochloric acid (HCl), pancreatin, bile, sodium hydroxide (NaOH), protease, Viscozyme (arabinase, cellulase, β-glucanase, hemicellulase and xylanase) were purchased from Sigma-Aldrich Corp. (St. Louis, MO, USA) and HPLC reagents were purchased from Merck KGaA (Darmstadt, Germany).
Viscozyme
Manufactured by Merck Group
Sourced in United States
Viscozyme is a multi-enzyme complex produced by Merck Group. It contains a wide range of carbohydrases, including cellulases, hemicellulases, pectinases, and other plant cell wall degrading enzymes. The core function of Viscozyme is to hydrolyze non-starch polysaccharides.
Automatically generated - may contain errors
Lab products found in correlation
Maltodextrin, by Merck Group (1 mentions)
Hydrochloric acid (hcl), by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Calcium chloride, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
α amylase, by Merck Group (1 mentions)
Folin ciocalteu, by Merck Group (1 mentions)
Cellulase, by Merck Group (1 mentions)
Xylanase, by Merck Group (1 mentions)
Hemicellulase, by Merck Group (1 mentions)
Gallic acid, by Merck Group (1 mentions)
β glucanase, by Merck Group (1 mentions)
Porcine pepsin, by Merck Group (1 mentions)
Calcium chloride dihydrate, by Merck Group (1 mentions)
Caffeine, by Merck Group (1 mentions)
Potassium persulfate, by Merck Group (1 mentions)
Porcine pancreatin, by Merck Group (1 mentions)
Caffeic, by Merck Group (1 mentions)
Gallic, by Merck Group (1 mentions)
Magnesium chloride hexahydrate, by Merck Group (1 mentions)
5 protocols using viscozyme
1
Antioxidant and Digestibility Assay
2
Phytochemical Standards and Analytical Reagents
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Methanol, sodium hydroxide, formic acid, acetonitrile, hydrochloride acid, sodium carbonate, ferric chloride hexahydrate, potassium chloride, sodium bicarbonate, sodium chloride, and Folin–Ciocalteu reagent were supplied by Panreac Química SLU (Barcelona, Spain). Pure phytochemical standards (≥96%), including gallic, caffeic, chlorogenic, p-coumaric, protocatechuic, and vanillic acids, rutin and quercetin, and caffeine, were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA), and extrasynthese (Genay, France). 6-Hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid (Trolox), 2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), potassium persulfate, 2,4,6-tris(2-pyridyl)-s-triazine (TPTZ), ammonium carbonate, calcium chloride dihydrate, magnesium chloride hexahydrate, potassium phosphate monobasic, porcine pepsin, porcine pancreatin, pronase E, and Viscozyme ® were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA).
3
Supercritical CO2 Extraction of Oregano
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O. vulgare spp. hirtum aerial parts were commercial samples purchased in the supermarket Diellas Markets (Corfu, Greece). The mean particle size (0.26 mm) of the material was determined using vibration sieve sets (CISA, Cedaceria, Spain). The carbon dioxide (Messer, Novi Sad, Serbia) with purity >99.98% (w/w) was used for the lab-scale scCO2 extraction. Viscozyme, a cellulolytic enzyme mixture (which contains a wide range of carbohydrases, including arabanase, cellulase, β-glucanase, hemicellulase, and xylanase), methanol, and 2,2-diphenyl-1-picrylhydrazyl (DPPH) were purchased from Sigma-Aldrich.
4
Viscozyme-Assisted Metabolite Extraction
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One leaf disc (Ø3 cm) was ground in an Eppendorf tube and mixed with 0.6 mL 50 mM sodium acetate, pH 5.3. To this, 20 µL of Viscozyme (Sigma-Aldrich) or water (control) was added prior to shaking for 1 h at room temperature. The Viscozyme treated sample appeared viscous compared to control. Metabolites were extracted in 1 mL ethyl acetate by shaking for 5 min and the upper layer was transferred to new vials. The solvent was evaporated under a stream of nitrogen and the residue re-suspended in 1 mL methanol and analyzed by LC–ESI–MS.
5
Enzymatic Hydrolysis of Pretreated Lignocellulosic Biomass
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Pretreated Corn Stover (PCS) or Pretreated Sugarcane Straw (PSCS) were prepared by steam explosion with diluted sulfuric acid at the Abengoa Bioenergy Biomass Pilot Plant in York (Iowa, USA), following the procedure described by Alcántara et al. (2016 (link)). Hydrolysis of PCS or PSCS (10 g l−1) was performed in 100 mL borosilicate conical flasks containing 1xM9 with pH adjusted to 5.5. The enzymatic cocktail Viscozyme (Sigma‐Merck) was added as indicated in Table S3 . Glucan content had been previously determined according to the standard biomass analytical procedures by NREL (Rocha‐Martín et al., 2018 ). Samples were incubated in an orbital incubator at 50 °C with shaking at 70 rpm for 24 h.
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