All experiments were performed using a total internal reflection fluorescence (TIRF) microscope, iMIC (Till Photonics, Germany), equipped with a 100x Olympus TIRF NA 1.49 DIC objective. The fluorophores were excited using laser lines at 488, 561 and 640 nm. The emitted fluorescence from the sample was filtered using an Andromeda quad-band bandpass filter (FF01-446-523-600-677). For dual-color experiments, an Andor TuCam beam splitter, equipped with a spectral long pass of 580 and 640nm and band pass filter of 525/50, 560/25, 710/80 (Semrock) was used. Time series were recorded using iXon Ultra 897 EMCCD Andor Cameras (X-8499 & X-8533) operating at 5 Hz frequency for standard acquisition and at 10 Hz for single molecule tracking.
Ixon ultra 897 emccd andor cameras
Manufactured by Oxford Instruments
The IXon Ultra 897 is an EMCCD (Electron Multiplying Charge Coupled Device) camera manufactured by Andor, a division of Oxford Instruments. It is designed for low-light scientific imaging applications that require high-sensitivity and fast frame rates.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ixon ultra 897 emccd andor cameras
1
Single Molecule Imaging with TIRF Microscopy
2
Single Molecule Imaging with TIRF Microscopy
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were performed using a total internal reflection fluorescence (TIRF) microscope, iMIC (Till Photonics, Germany), equipped with a 100x Olympus TIRF NA 1.49 DIC objective. The fluorophores were excited using laser lines at 488, 561 and 640 nm. The emitted fluorescence from the sample was filtered using an Andromeda quad-band bandpass filter (FF01-446-523-600-677). For dual-color experiments, an Andor TuCam beam splitter, equipped with a spectral long pass of 580 and 640nm and band pass filter of 525/50, 560/25, 710/80 (Semrock) was used. Time series were recorded using iXon Ultra 897 EMCCD Andor Cameras (X-8499 & X-8533) operating at 5 Hz frequency for standard acquisition and at 10 Hz for single molecule tracking.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!