IHC was performed on paraffin-embedded sections. The sections were deparaffinized in xylene and hydrated with decreasing concentrations of ethanol (100, 90, 80, 75%) for 3 min each time and microwaved-heated in sodium citrate buffer for antigen retrieval. Then, the sections were blocked in 5% BSA and incubated with anti- IL-7 rabbit polyclonal antibody (1:100, R&D Systems, MN, USA) at 4 °C overnight. Next, the sections were treated with horseradish peroxidase (HRP)‑conjugated rabbit secondary antibody (1:200; ProteinTech Group) for 60 min at room temperature; then, 3,3′‑diaminobenzidine development (DAB Substrate Chromogen System; Dako) and hematoxylin staining were performed. The sections were fixed and images were obtained with inverted microscope (Olympus IX71, Japan).
Horseradish peroxidase hrp conjugated rabbit secondary antibody
Manufactured by Proteintech
Sourced in Denmark
Horseradish peroxidase (HRP)-conjugated rabbit secondary antibody is a laboratory reagent used for the detection and visualization of target proteins in various immunoassays. The HRP enzyme acts as a reporter molecule, catalyzing a colorimetric or chemiluminescent reaction to generate a signal that can be measured. This secondary antibody is specific to rabbit primary antibodies, providing a sensitive and reliable tool for protein analysis.
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Lab products found in correlation
4 protocols using horseradish peroxidase hrp conjugated rabbit secondary antibody
1
Immunohistochemical Detection of IL-7
2
Immunohistochemical Analysis of Tissue Samples
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IHC was performed on paraffin-embedded sections. The sections were deparaffinized in xylene and hydrated with decreasing concentrations of ethanol (100, 90, 80, 75%) for 3 min each time and microwaved-heated in sodium citrate buffer for antigen retrieval. Then, the sections were blocked in 5% BSA and incubated with anti-IGF2BP3, VEGF, CD31, Ki67 rabbit polyclonal antibody (1:200; ProteinTech Group, Inc., Wuhan, China) at 4 °C overnight. Next, the sections were treated with horseradish peroxidase (HRP)-conjugated rabbit secondary antibody (1:200; ProteinTech Group, Inc.) for 60 min at room temperature; then, 3,3′-diaminobenzidine development (DAB Substrate Chromogen System; Dako, Denmark) and hematoxylin staining were performed. The sections were fixed and images were obtained with inverted microscope (Olympus IX71, Japan).
3
Immunohistochemical Evaluation of Cancer Markers
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IHC was performed on para n-embedded sections. The sections were depara nized in xylene and hydrated with decreasing concentrations of ethanol (100, 90, 80, 75%) for 3 min each time and microwaved-heated in sodium citrate buffer for antigen retrieval. Then, the sections were blocked in 5% BSA and incubated with anti-IGF2BP3, VEGF, CD31, Ki67 rabbit polyclonal antibody (1:200; ProteinTech Group, Inc., Wuhan, China) at 4°C overnight. Next, the sections were treated with horseradish peroxidase (HRP)-conjugated rabbit secondary antibody (1:200; ProteinTech Group, Inc.) for 60 min at room temperature; then, 3,3'-diaminobenzidine development (DAB Substrate Chromogen System; Dako, Denmark) and hematoxylin staining were performed. The sections were xed and images were obtained with inverted microscope (Olympus IX71, Japan).
4
Immunohistochemistry Profiling of Tumor Markers
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IHC was performed on para n-embedded sections. The sections were depara nized in xylene and hydrated with decreasing concentrations of ethanol (100, 90, 80, 75%) for 3 min each time and microwaved-heated in sodium citrate buffer for antigen retrieval. Then, the sections were blocked in 5% BSA and incubated with anti-IGF2BP3, VEGF, CD31, Ki67 rabbit polyclonal antibody (1:200; ProteinTech Group, Inc., Wuhan, China) at 4 °C overnight. Next, the sections were treated with horseradish peroxidase (HRP)-conjugated rabbit secondary antibody (1:200; ProteinTech Group, Inc.) for 60 min at room temperature; then, 3,3'-diaminobenzidine development (DAB Substrate Chromogen System; Dako, Denmark) and hematoxylin staining were performed. The sections were xed and images were obtained with inverted microscope (Olympus IX71, Japan).
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