Antibodies used in this study include rabbit anti-ephrinB2 (PA5-35062; Thermo Fisher Scientific), mouse anti-EphB4 (H-10) (sc-365510; Santa Cruz Biotechnology), mouse anti-flag (F3165; Sigma-Aldrich), goat anti-DsRed (sc-33354; Santa Cruz Biotechnology), mouse monoclonal anti-p-Tyr (sc-508; Santa Cruz Biotechnology), mouse anti-paxillin (610051; BD Transduction Laboratories), and anti-α-tubulin (T6074; Sigma-Aldrich). Mouse anti-HA (12CA5) and rabbit anti-EGFP antibodies were developed in the laboratory. Rabbit anti-HA (923501) and anti-DYKDDDDK Tag (L5) Affinity Gel (651502) antibodies were purchased from BioLegend. Secondary Alexa Fluor–labeled antibodies were from Jackson ImmunoResearch. Protein G (L00209) resin was from GenScript. Recombinant mouse EphB4 (extracellular domain residue 16-539)-Fc protein (446-B4-200) was purchased from R&D Systems, Inc.
Alexa fluor labeled antibodies
Manufactured by Jackson ImmunoResearch
Sourced in Panama
Alexa Fluor–labeled antibodies are fluorescent-labeled antibodies produced by Jackson ImmunoResearch. They are designed for use in various immunoassay and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Mouse anti paxillin, by BD (1 mentions)
Mouse anti flag f3165, by Merck Group (1 mentions)
Goat anti dsred, by Santa Cruz Biotechnology (1 mentions)
Anti α tubulin t6074, by Merck Group (1 mentions)
Efluor 670, by Thermo Fisher Scientific (1 mentions)
Carboxyfluorescein succinimidyl ester (cfse), by Thermo Fisher Scientific (1 mentions)
Dnase 1, by Roche (1 mentions)
Foxp3 transcription factor fixation permeabilization kit, by Thermo Fisher Scientific (1 mentions)
Brefeldin a, by BioLegend (1 mentions)
Mouse cd4 t cell isolation kit, by BioLegend (1 mentions)
Anti madcam 1 meca367, by BioLegend (1 mentions)
Monensin, by BioLegend (1 mentions)
Liberase tl research grade, by Roche (1 mentions)
Ionomycin, by BioLegend (1 mentions)
Mojosort mouse cd3 t cell isolation kit, by BioLegend (1 mentions)
2 protocols using alexa fluor labeled antibodies
1
Antibody Profiling for Ephrin-Eph Signaling
2
Isolation and Characterization of Mouse T Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The following antibodies were from BioLegend (San Diego, CA): CD4 (GK1.5), β7 (FIB504), Foxp3 (MF-14), anti-CD3 (2C11), anti-CD28 (37.51), IL10 (JES5-16E3), TGF-β1 (TW7-16B4), and anti–MAdCAM-1 (MECA367). Secondary AlexaFluor-labeled antibodies were from Jackson ImmunoResearch (West Grove, PA). The Foxp3 transcription factor fixation/permeabilization kit was purchased from eBioscience. CFSE and eFluor670 were purchased from Invitrogen (Carlsbad, CA) and BioLegend, respectively. Piroxicam was from MilliporeSigma (Burlington, MA). Ionomycin, brefeldin A, and monensin were from BioLegend. The MojoSort mouse CD3 T-cell isolation kit and mouse CD4 T-cell isolation kit were from BioLegend. Liberase TL (research grade) and DNAse I were from Roche (Basel, Switzerland). Recombinant mouse MAdCAM-1–Fc was purified by protein A beads as previously described.49 (link)
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