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C myc transcription factor assay kit

Manufactured by Abcam
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Sourced in United Kingdom

The C-Myc transcription factor assay kit is a tool used to detect and quantify the levels of the C-Myc protein, a transcription factor that plays a key role in regulating cell growth and proliferation. The kit provides a reliable and efficient method for measuring C-Myc activity in cell lysates or nuclear extracts.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (2 mentions) Versamax, by Molecular Devices (1 mentions) C myc, by Abcam (1 mentions)

Close spelling variants of this product

Transcription Factor Assay Kit C-Myc

4 protocols using c myc transcription factor assay kit

1

Quantifying c-MYC Transcriptional Activity

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The transcriptional activity of c-MYC was investigated in protein nuclear extracts from human HCC samples (n = 42) using the c-MYC Transcription Factor Assay Kit (Abcam, Cambridge, United Kingdom), following the manufacturer’s protocol. Nuclear extracts were isolated from frozen specimens using the NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher Scientific). Experiments were repeated three times in triplicate.
Jia J., Che L., Cigliano A., Wang X., Peitta G., Tao J., Zhong S., Ribback S., Evert M., Chen X, & Calvisi D.F. (2020). Pivotal Role of Fatty Acid Synthase in c-MYC Driven Hepatocarcinogenesis. International Journal of Molecular Sciences, 21(22), 8467.
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2

Assessing c-MYC Transcription Factor Inhibition

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The protocol was adapted from the Abcam c-MYC Transcription Factor Assay kit (ab207200). 50 mM DMSO stock solutions of compound were diluted into dPBS then vortexed. 0.2 μg of c-MYC (Abcam, ab169901) was added to each Eppendorf tube then mixed gently. c-MYC was incubated with compound or DMSO control for 30 minutes at 37 °C. After incubation, 0.2 μg of MAX (Origene, TP306812) was added to each Eppendorf tube then mixed gently. Samples were added to 96-well plate containing the consensus sequence for MYC/MAX binding. Experiment proceeded following manufacturer’s protocol for binding, primary antibody, and secondary antibody incubation steps. After addition of developing and stop solution, the OD450 was read on a Molecular Devices VersaMax microplate reader. Data was normalized to DMSO control after subtracting blank wells.
Boike L., Cioffi A.G., Majewski F.C., Co J., Henning N.J., Jones M.D., Liu G., McKenna J.M., Tallarico J.A., Schirle M, & Nomura D.K. (2020). Discovery of a Functional Covalent Ligand Targeting an Intrinsically Disordered Cysteine Within MYC. Cell chemical biology, 28(1), 4-13.e17.
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3

Quantifying c-Myc Activation in Gastric Cancer

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The c-Myc transcription factor assay Kit (Abcam, ab207200) was used to quantify c-Myc activation in nuclear extracts from gastric cancer cells. In brief, HGC-27 and MGC-803 cells were subjected to treatment with 5 µM FTY-720 or 10 µM DT-061 for 24h, followed by detection of c-Myc transcriptional activity in according to the manufacturer's instructions.
Cai Z., Zhang W., Zhou R., Wang Y, & Feng Y. (2023). Protein Phosphatase 2a Inhibits Gastric Cancer Cell Glycolysis by Reducing MYC Signaling. Cell biochemistry and biophysics, 81(1).
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4

Quantifying c-Myc Activation in Gastric Cancer

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The c-Myc transcription factor assay Kit (Abcam, ab207200) was used to quantify c-Myc activation in nuclear extracts from gastric cancer cells. In brief, HGC-27 and MGC-803 cells were subjected to treatment with 5 µM FTY-720 or 10 µM DT-061 for 24h, followed by detection of c-Myc transcriptional activity in according to the manufacturer's instructions.
Zhang W., Cai Z., Zhou R., Liu X., Wang Y., & Feng Y. (2021). Protein Phosphatase 2A Inhibits Gastric Cancer Cell Glycolysis by Reducing MYC Signaling.
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