Blood samples were collected from fasting participants using tubes without an anticoagulant and centrifuged at 54 g for 20 min at 4°C. The serum was separated, aliquoted and stored at −80°C until analysis. Biochemical assays on the serum were performed at the Department of Biochemistry in the Central Hospital of Army, Algiers. Serum folate and vitamin B12 concentrations were measured by competitive binding assays with electro‐chemiluminescent detection using an automate (Elecsys 2010, Hitachi, Roche or COBASe401), while serum total homocysteine (tHcy) was analyzed through automated fluorescence polarization immunoassay (ARCHITECT system immunoassay, Abbott Laboratories).
Cobase401
Manufactured by Roche
Sourced in Germany
The COBASe401 is a modular analytical system designed for clinical laboratories. It is a fully automated instrument that performs a range of diagnostic tests, including immunoassay and clinical chemistry analyses. The COBASe401 offers high-throughput capabilities and is optimized for efficient and reliable sample processing.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using cobase401
1
Serum Biomarkers for Nutritional Status
2
Cord Blood Vitamin D and Mineral Levels
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Following delivery, three milliliters of cord blood were collected from infants who met the study criteria to determine serum concentrations of 25(OH)D, calcium (Ca), magnesium (Mg), phosphorus (P), albumin, parathormone (PTH), and alkaline phosphatase (ALP). Blood samples were collected and sent to the laboratory in accordance with the cold chain protocol. Calcium, P, Mg, albumin, and ALP levels in serum samples were measured using enzymatic, kinetic, and end-point methods on a Beckman Coulter AU2700 analyser with original reagents (Brea, CA, USA). The electrochemiluminescence method was used on an analyzer to measure serum PTH levels (Beckman Coulter DXI Brea, CA, USA), as well as 25(OH)D (Roche Diagnostics Cobas e401 GMBH Mannheim, Germany). The global consensus defines vitamin D deficiency and insufficiency in children as <12 and 12–20 ng/mL, respectively, in the prevention and treatment of nutrition-related rickets [17 (link)]. Based on this consensus, three groups were formed based on cord 25(OH) D vitamin levels; Group 1: 25(OH)D < 12 ng/mL (Deficient); Group 2: 25(OH)D = 12–19 ng/mL (Insufficient); and Group 3: 25(OH)D = 20–100 ng/mL (Optimum).
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