Western blot analysis was performed as described previously36 (link). The primary antibodies were as follows: anti-p27 (ab193379, Abcam), anti-phosphorylated Rb (Ser795) (#9301, Cell Signaling Technology), anti-E2F1 (#3742, Cell Signaling Technology), anti-p21 (#2947, Cell Signaling Technology), anti-caspase-3 antibody (ab32351, Epitomics), anti-FHL2 (ab12327, Abcam), anti-β-catenin (#8480, Cell Signaling Technology), anti-phosphorylated β-catenin (Ser33/37/Thr41) (#9561, Cell Signaling Technology), anti-cyclin D1 (60186-lg, Proteintech), anti-p53 (10442-1-AP, Proteintech), anti-PUMA (ab33906, Epitomics), anti-E-cadherin (20874-1-AP, Proteintech), anti-N-cadherin (22018-1-AP, Proteintech), and anti-GAPDH (#2118, Cell Signaling Technology).
Anti phosphorylated β catenin ser33 37 thr41
Manufactured by Cell Signaling Technology
Anti-phosphorylated β-catenin (Ser33/37/Thr41) is a primary antibody that recognizes β-catenin when phosphorylated at serine 33, serine 37, and threonine 41. This antibody is used to detect the phosphorylated form of β-catenin, which plays a role in the Wnt signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Anti gapdh, by Cell Signaling Technology (3 mentions)
Anti β catenin, by Cell Signaling Technology (2 mentions)
Pvdf membrane, by Bio-Rad (2 mentions)
Ab193379, by Abcam (1 mentions)
Ab32351, by Abcam (1 mentions)
Ab12327, by Abcam (1 mentions)
Anti e2f1, by Cell Signaling Technology (1 mentions)
Anti n cadherin 22018 1 ap, by Proteintech (1 mentions)
Anti phosphorylated rb ser795, by Cell Signaling Technology (1 mentions)
Anti cyclin d1 60186 lg, by Proteintech (1 mentions)
Anti e cadherin 20874 1 ap, by Proteintech (1 mentions)
Anti p21, by Cell Signaling Technology (1 mentions)
Anti p53, by Proteintech (1 mentions)
Anti p53 10442 1 ap, by Proteintech (1 mentions)
Anti ki67, by Cell Signaling Technology (1 mentions)
Anti cyclind1 ab16663, by Abcam (1 mentions)
Anti rspo2 17781 1 ap, by Proteintech (1 mentions)
Ecl plus reagent, by Bio-Rad (1 mentions)
3 protocols using anti phosphorylated β catenin ser33 37 thr41
1
Western Blot Analysis of Cell Signaling Proteins
2
Comprehensive Protein Analysis of GC Cells
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GC cells were lysed by RIPA buffer for 20min at 0° C. The proteins were processed by SDS-PAGE and transferred to PVDF membrane (BioRad). The primary antibodies used in Western blotting were as follows: anti-TIMP1 (ab211926; Abcam), anti-β-catenin (#8480; Cell Signaling Technology), anti-Ki67 (#9449; Cell Signaling Technology), anti-GAPDH (2188, Cell Signaling Technology), anti-p53 (10442-1-AP, Proteintech) and anti-phosphorylated β-catenin (Ser33/37/Thr41) (#9561, Cell Signaling Technology).
3
Protein Expression Analysis in Cells
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The cells were lysed in RIPA buffer for 20 minutes at 0 °C. SDS-PAGE was used to isolate the proteins and transfer them to a PVDF membrane (BioRad). Primary antibodies (1:2001000) were used to incubate the membranes, followed by HRP-conjugated secondary antibodies. ECL plus reagents were used to detect protein bands (Bio-Rad). Primary antibodies used were anti-RSPO2 (17781-1-AP; Proteintech), anti-β-cantenin (8480S; Cell Signaling Technology), anti-phosphorylated β-catenin (Ser33/37/Thr41) (#9561, Cell Signaling Technology), anti-CyclinD1 (ab16663; Abcam) and anti-GAPDH (2188; Cell Signaling Technology).
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