Simoa human nfl assay

Manufactured by Quanterix

The Simoa Human NfL assay is a laboratory tool designed to measure the concentration of Neurofilament Light (NfL) protein in human samples. NfL is a biomarker that can provide insights into neurological health and disease. The assay utilizes Quanterix's Simoa technology to enable highly sensitive and precise quantification of NfL levels.

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Lab products found in correlation

Nf light elisa, by UmanDiagnostics (1 mentions) Simoa hd 1 analyser, by Quanterix (1 mentions) Innotest elisa, by Fujirebio (1 mentions) Simoa hd 1 analyzer, by Quanterix (1 mentions)

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Simoa assay (17 citations) Simoa NfL assay (3 citations)

2 protocols using simoa human nfl assay

Biomarker Assessment in Neurodegenerative Disorders

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Out of 60 patients, 14 had CSF and 30 had plasma collected. CSF amyloid‐beta (1–42), p‐tau and t‐tau levels were determined by INNOTEST ELISAs (Fujirebio); CSF NfL was determined using NF‐Light ELISA (UmanDiagnostics), according to the manufacturer's instructions. Plasma NfL levels were measured using ultrasensitive single molecule array (Simoa) Human NfL assay and Simoa HD‐1 Analyser (Quanterix, MA), according to the manufacturer's protocol. Serum progranulin levels were measured using Progranulin ELISA (Adipogen).

Tan Y.J., Yong A.C., Foo J.N., Lian M.M., Lim W.K., Dominguez J., Fong Z.H., Narasimhalu K., Chiew H.J., Ng K.P., Ting S.K., Kandiah N, & Ng A.S. (2023). C9orf72 expansions are the most common cause of genetic frontotemporal dementia in a Southeast Asian cohort. Annals of Clinical and Translational Neurology, 10(4), 568-578.

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Plasma Neurofilament Light in Parkinson's Disease

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Blood samples were collected at baseline and annually. A total of 199 participants (149 PD and 50 HC) had their plasma NfL levels measured at baseline. Seventy-six PD patients (30 TD and 46 PIGD) had plasma NfL measured at the 2-year mark. EDTA blood was centrifuged at 1500 g for 15 min within 1 h after collection. Plasma was aliquoted and stored at − 80 °C until further analysis. Samples were thawed only once for NfL measurement. NfL levels were measured using ultrasensitive single molecule array (Simoa) Human NfL assay and Simoa HD-1 Analyzer (Quanterix, MA), according to the manufacturer’s protocol. Coefficient of variation of samples measured in duplicates was < 10%. Two quality-control samples with high and low NfL concentrations, provided in the kit, were measured in each run and were in the expected range.

Ng A.S., Tan Y.J., Yong A.C., Saffari S.E., Lu Z., Ng E.Y., Ng S.Y., Chia N.S., Choi X., Heng D., Neo S., Xu Z., Keong N.C., Tay K.Y., Au W.L., Tan L.C, & Tan E.K. (2020). Utility of plasma Neurofilament light as a diagnostic and prognostic biomarker of the postural instability gait disorder motor subtype in early Parkinson’s disease. Molecular Neurodegeneration, 15, 33.

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