Opti-MEM (31985088) was purchased from Gibco. MitoTracker Red CMXRos (40740ES50) was purchased from YEASEN. DAPI (C1002) and N-acetylcysteine (S0077) were purchased from Beyotime. 3-methyladenine (3-MA) (S2767) and bafilomycin A1 (Baf-A1) (S1413) were purchased from Selleck Chemicals. CCCP (C2759) (SAB5701328) was purchased from Sigma-Aldrich. Anti-Occludin (91131S), Claudin-1 (13255S), GAPDH (5174S), Parkin(2132S), and β-actin (3700S) were purchased from Cell Signaling Technology. Anti-P62 (T55546), ATG5 (T55766), and LC3B primary antibodies were purchased from Abmart.
Cccp c2759
Manufactured by Merck Group
Sourced in India, United States
CCCP (C2759) is a chemical compound that functions as an uncoupling agent in cellular respiration. It disrupts the proton gradient across the inner mitochondrial membrane, which is a key step in the production of ATP. This product is commonly used in biochemical research to investigate mitochondrial function and energy metabolism.
Automatically generated - may contain errors
Lab products found in correlation
N acetylcysteine, by Beyotime (1 mentions)
Dapi c1002, by Beyotime (1 mentions)
Mitotracker red cmxros, by Yeasen (1 mentions)
Bafilomycin a1 baf a1, by Selleck Chemicals (1 mentions)
Opti mem, by Thermo Fisher Scientific (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Ethidium bromide, by Merck Group (1 mentions)
Mueller hinton agar (mha), by HiMedia (1 mentions)
Luria bertani broth, by HiMedia (1 mentions)
Anti srebp 1 sc 13551, by Santa Cruz Biotechnology (1 mentions)
Anti caspase 3 d3r6y, by Cell Signaling Technology (1 mentions)
Baf a1 s1413, by Selleck Chemicals (1 mentions)
Anti β actin 66009 1 ig, by Proteintech (1 mentions)
Anti acaca 21923 1 ap, by Proteintech (1 mentions)
Mitochondrial membrane potential assay kit c2006, by Beyotime (1 mentions)
Cell culture reagents, by Thermo Fisher Scientific (1 mentions)
Qlaamp dna mini kit, by Qiagen (1 mentions)
Cfx96 touch real time pcr detection system, by Bio-Rad (1 mentions)
5 protocols using cccp c2759
1
Autophagy Regulation in Cell Assays
2
Antibiotic Resistance Profiling
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Mueller–Hinton agar and Luria–Bertani broth were obtained from Himedia (Mumbai, India), tigecycline antibiotic powder was obtained from Cipla Ltd., (Mumbai, India), and CCCP (C2759) and ethidium bromide were obtained from Sigma Aldrich, St. Louis, MO, USA).
3
Mitochondrial Regulation and Apoptosis
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Anti-α-tubulin (YM3035) and anti-β-tubulin (YM3139) were purchased from ImmunoWay Biotechnology (TX, USA); anti-FASN (SC-48357), anti-NIX (SC-166332), and anti-SREBP1 (SC-13551) were purchased from Santa Cruz Biotech (TX, USA); anti-BNIP3 (D121876) and anti-ACLY (D221957) were purchased from Sangon Biotech(Shanghai, China); anti-ACACA (21923-1-AP) and anti-β-actin (66009-1-Ig) were purchased from Proteintech Group (IL, USA); anti-LC3 II/I (D3U4C) and anti-caspase-3 (D3R6Y) were purchased from Cell Signaling Technology (MA, USA); anti-VDAC1 (AB14734) was purchased from Abcam (MA, USA); D, L-Sulforaphane (574215) was purchased from Sigma-Aldrich (MO, USA); CCCP (C2759) was purchased from Sigma-Aldrich (MO, USA); Baf-A1 (S1413) and PS-341 (S1013) were purchased from Selleck (Shanghai, China); Cell Mitochondria Isolation Kit (C3601), ROS Assay Kit (S0033), and Mitochondrial Membrane Potential Assay Kit (C2006) were purchased from Beyotime Biotechnology (Shanghai, China).
4
Autophagy-Related Protein Detection Protocols
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The following antibodies were used: monoclonal anti-P62/SQSTM1 (Santa Cruz, sc-28359; dilutions: IF/P-LISA 1/200, WB 1/1000), polyclonal anti-LC3B (Sigma, L8918; dilutions: IF/P-LISA 1/100, WB 1/3000), polyclonal anti-GABARAPL1 (Proteintech, 11010-AP, dilution: IF/P-LISA 1/100), monoclonal anti-NIX (Santa Cruz, sc-166332; dilution: IF/P-LISA 1/100), polyclonal anti-rabbit-HRP conjugate (P.A.R.I.S, BI2407; dilution: 1/10 000), polyclonal anti-mouse-HRP conjugate (P.A.R.I.S.; dilution: 1/10 000), polyclonal anti-rabbit-Alexa 488 (Life Technologies, BI2413C; dilution 1/1 000), and polyclonal anti-mouse-Alexa 555 (Life Technlogies; dilution: 1/1000). Cell culture reagents were purchased from Invitrogen. EBSS (E3024), BafA1 (B1793), Rapa (R8781) and CCCP (C2759) were purchased from Sigma-Aldrich. Rot (AC13237) was purchased from Acros organics.
5
Mitochondrial DNA Copy Number Quantification
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Genomic DNA was purified with a QlAamp® DNA Mini Kit (Qiagen GmbH, Hilden, Germany). qPCR was performed with a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). mtDNA copy number was estimated using ∆Cq of mtDNA(ND1)/nDNA(18 S rRNA), as previously described8 (link). Mitochondria inhibitors, ddC (D5782), rotenone (R8875), and CCCP (C2759), were purchased from Sigma-Aldrich.
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