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Novaseq 6000 lane

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The NovaSeq 6000 lane is a component of the NovaSeq 6000 Sequencing System, a high-throughput DNA sequencing instrument developed by Illumina. The lane is responsible for holding and processing samples during the sequencing process.

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Lab products found in correlation

Nebnext ultra 2 rna library prep with sample purification beads, by New England Biolabs (1 mentions) Qubit dsdna br assay kit, by Thermo Fisher Scientific (1 mentions) Nebnext poly a mrna magnetic isolation module, by New England Biolabs (1 mentions) 2100 bioanalyzer, by Agilent Technologies (1 mentions)

3 protocols using novaseq 6000 lane

1

Ancient DNA Sequencing Protocol

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Samples AL2657, AL2541, AL2741, AL2744, AL3185, AL2350, CH1109, AL2370, AL3272 and AL3284 were processed at the dedicated ancient DNA facility at the PalaeoBARN laboratory at the University of Oxford, following methods described previously8 (link). In brief, double-stranded libraries were constructed following the protocol in ref. 48 . These libraries were sequenced on a HiSeq 2500 (AL2657, AL2541, AL2741, AL2744) or a HiSeq 4000 (AL3185, AL2350, CH1109) instrument at the Danish National Sequencing Center or on a NextSeq 550 instrument (AL2741) at the Natural History Museum of London. For samples AL2370, AL3272 and AL3284, between six and eight separate PCR reactions with unique indexes were carried out on their libraries and they were sequenced alongside samples HOV4, VAL_18A and IN18_016 on an Illumina NovaSeq 6000 lane with an S4 100-bp paired-end set-up at SciLifeLab in Stockholm.
Bergström A., Stanton D.W., Taron U.H., Frantz L., Sinding M.H., Ersmark E., Pfrengle S., Cassatt-Johnstone M., Lebrasseur O., Girdland-Flink L., Fernandes D.M., Ollivier M., Speidel L., Gopalakrishnan S., Westbury M.V., Ramos-Madrigal J., Feuerborn T.R., Reiter E., Gretzinger J., Münzel S.C., Swali P., Conard N.J., Carøe C., Haile J., Linderholm A., Androsov S., Barnes I., Baumann C., Benecke N., Bocherens H., Brace S., Carden R.F., Drucker D.G., Fedorov S., Gasparik M., Germonpré M., Grigoriev S., Groves P., Hertwig S.T., Ivanova V.V., Janssens L., Jennings R.P., Kasparov A.K., Kirillova I.V., Kurmaniyazov I., Kuzmin Y.V., Kosintsev P.A., Lázničková-Galetová M., Leduc C., Nikolskiy P., Nussbaumer M., O’Drisceoil C., Orlando L., Outram A., Pavlova E.Y., Perri A.R., Pilot M., Pitulko V.V., Plotnikov V.V., Protopopov A.V., Rehazek A., Sablin M., Seguin-Orlando A., Storå J., Verjux C., Zaibert V.F., Zazula G., Crombé P., Hansen A.J., Willerslev E., Leonard J.A., Götherström A., Pinhasi R., Schuenemann V.J., Hofreiter M., Gilbert M.T., Shapiro B., Larson G., Krause J., Dalén L, & Skoglund P. (2022). Grey wolf genomic history reveals a dual ancestry of dogs. Nature, 607(7918), 313-320.
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2

Dhole Tooth Sequencing Protocol

Cited 2 times
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Sample HOV4 was processed at the Department of Anthropology, University of Vienna. The sample is a canine tooth, which after sequencing was determined to derive from a dhole (Cuon alpinus). DNA was extracted from its cementum using the methods described in ref. 63 (link) with a modified incubation time of ~18 h. The library was prepared according to the protocol in ref. 48 with the modifications from ref. 64 (link). Five separate PCR reactions with unique indexes were carried out on the library and were sequenced alongside samples VAL_18A, IN18_016, AL2242, AL2370, AL2893, AL3272 and AL3284 on an Illumina NovaSeq 6000 lane with an S4 100-bp paired-end set-up at SciLifeLab in Stockholm.
An overview of all samples and their associated metadata is available in Supplementary Data 1.
Bergström A., Stanton D.W., Taron U.H., Frantz L., Sinding M.H., Ersmark E., Pfrengle S., Cassatt-Johnstone M., Lebrasseur O., Girdland-Flink L., Fernandes D.M., Ollivier M., Speidel L., Gopalakrishnan S., Westbury M.V., Ramos-Madrigal J., Feuerborn T.R., Reiter E., Gretzinger J., Münzel S.C., Swali P., Conard N.J., Carøe C., Haile J., Linderholm A., Androsov S., Barnes I., Baumann C., Benecke N., Bocherens H., Brace S., Carden R.F., Drucker D.G., Fedorov S., Gasparik M., Germonpré M., Grigoriev S., Groves P., Hertwig S.T., Ivanova V.V., Janssens L., Jennings R.P., Kasparov A.K., Kirillova I.V., Kurmaniyazov I., Kuzmin Y.V., Kosintsev P.A., Lázničková-Galetová M., Leduc C., Nikolskiy P., Nussbaumer M., O’Drisceoil C., Orlando L., Outram A., Pavlova E.Y., Perri A.R., Pilot M., Pitulko V.V., Plotnikov V.V., Protopopov A.V., Rehazek A., Sablin M., Seguin-Orlando A., Storå J., Verjux C., Zaibert V.F., Zazula G., Crombé P., Hansen A.J., Willerslev E., Leonard J.A., Götherström A., Pinhasi R., Schuenemann V.J., Hofreiter M., Gilbert M.T., Shapiro B., Larson G., Krause J., Dalén L, & Skoglund P. (2022). Grey wolf genomic history reveals a dual ancestry of dogs. Nature, 607(7918), 313-320.
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3

Multiplexed RNA-seq Library Preparation

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RNAseq libraries for JU1373 and NIC58 were prepared simultaneously from mRNA isolated from 1 µg of pooled total RNA using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). RNA fragmentation, first and second strand cDNA synthesis, and end-repair processing were performed with the NEBNext Ultra II RNA Library Prep with Sample Purification Beads (New England Biolabs). Adapters and unique dual indexes in the NEBNext Multiplex Oligos for Illumina (New England Biolabs) were ligated, and the concentration of each library was determined using Qubit dsDNA BR Assay Kit (Invitrogen). Libraries were pooled and qualified by Bioanalyzer 2100 (Agilent; Novogene, CA, USA), and 150 bp paired-end reads were sequenced on a single Illumina NovaSeq 6000 lane.
Noble L.M., Yuen J., Stevens L., Moya N., Persaud R., Moscatelli M., Jackson J.L., Zhang G., Chitrakar R., Baugh L.R., Braendle C., Andersen E.C., Seidel H.S, & Rockman M.V. (2021). Selfing is the safest sex for Caenorhabditis tropicalis. eLife, 10, e62587.
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