Passage-1 cells (1 × 106 cells) were suspended in 100 μl phosphate buffered saline (PBS) containing 10 μg/ml antibodies for APC-conjugated CD90 (Becton Dickinson, Franklin Lakes, NJ, USA), PE-conjugated CD44 (eBioscience, San Diego, CA, USA), FITC-conjugated CD45 (eBioscience), and PerCP-Cyanine5.5-conjugated CD11b (Biolegend, San Diego, CA, USA). As an isotype control, nonspecific anti-mouse IgG coupled with APC, PE, FITC, or PerCP-Cyanine5.5 (Becton Dickinson) was substituted for the primary antibody. After incubation for 30 minutes at 4°C, the cells were washed with PBS and then resuspended in 500 μl of PBS for analysis. Cell fluorescence was evaluated by flow cytometry using a FACSVerse instrument (Becton Dickinson); the data were analyzed using Flowjo software (Tree Star, Ashland, OR, USA). All analyses were performed on samples from all 6 donors. The data were analyzed with the Kruskal Wallis test, followed by Dunn’s multiple comparisons test.
Fitc conjugated cd45
Manufactured by Thermo Fisher Scientific
Sourced in United States
FITC-conjugated CD45 is a fluorescent-labeled antibody that binds to the CD45 antigen, which is expressed on the surface of various leukocyte populations. It is a common marker used in flow cytometry applications for the identification and analysis of different blood cell types.
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Lab products found in correlation
Pe conjugated cd44, by Thermo Fisher Scientific (1 mentions)
Facsverse, by BD (1 mentions)
Fluorescein isothiocyanate (fitc), by BD (1 mentions)
Apc conjugated cd90, by BD (1 mentions)
Cup filcons, by BD (1 mentions)
Facsaria 3, by BD (1 mentions)
Facscanto 2, by BD (1 mentions)
Cd45ra bv605, by BioLegend (1 mentions)
Fluorescein isothiocyanate (fitc), by Thermo Fisher Scientific (1 mentions)
Tryple, by Thermo Fisher Scientific (1 mentions)
2 protocols using fitc conjugated cd45
1
Immunophenotyping of Passage-1 Cells
2
Multiparametric Flow Cytometry Profiling
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Differentiated cells were washed in PBS before being singularized using TrypLE (Thermo Fischer Scientific), passed through a 21G needle, and filtered using 30 μm sterile Cup Filcons (BD Biosciences). Cells were treated with 7AAD to exclude dead cells. Cells were stained using the following anti-human antibodies; fluorescein isothiocyanate (FITC)-conjugated CD45 (eBioscience, 11-0459-42) and CD43 (BD Biosciences, 555475), phycoerythrin-cyanine (PE-Cy7)-conjugated CD34 (BioLegend, 343516), allophycocyanin-conjugated CD38 (BioLegend, 303510), CD33 (BioLegend, 303408), CD14 (BioLegend, 325608) Phycoerythrin (PE)-conjugated CD90 (BioLegend, 328110), CD19 (BioLegend, 302208), CD7 (BD Biosciences, 332774), V450-conjugated CD45RA (BD Biosciences, 560362), PE-Cy5-CD56 (BioLegend, 304608), BV605-CD45RA (BioLegend, 304133), and eFluor450-CD16 (eBioscience, 48-0168-42). Cells were acquired on a FACS Canto II (BD Biosciences) or sorted using a FACS Aria III (BD Biosciences). Analysis was done using FlowJo, version 9.4.10 (BD Biosciences). FACS gates are based on fluorescence minus one (FMO) controls unless stated otherwise.
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