Cgp 54626 hydrochloride

Manufactured by Bio-Techne

Sourced in United Kingdom

CGP 54626 hydrochloride is a chemical compound commonly used in research applications. It is a selective and potent antagonist of the GABAB receptor. The core function of this product is to inhibit GABAB receptor activity, which can be utilized in various experimental settings to study the physiological and pharmacological effects of GABAB receptor modulation.

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Lab products found in correlation

6 protocols using cgp 54626 hydrochloride

Serotonin Receptor Pharmacology Assay

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Serotonin hydrochloride, MDL 72,222, RS 102221, MDL 100,907, SR95531 and CGP 54626 hydrochloride were purchased from Tocris Bioscience UK. Picrotoxin, kynurenic acid and α-Methylserotonin maleate salt were purchased from Sigma-Aldrich UK.

Bocchio M., Fucsina G., Oikonomidis L., McHugh S.B., Bannerman D.M., Sharp T, & Capogna M. (2015). Increased Serotonin Transporter Expression Reduces Fear and Recruitment of Parvalbumin Interneurons of the Amygdala. Neuropsychopharmacology, 40(13), 3015-3026.

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Pharmacological Tools for Neuroscience Research

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4-[3-[2-(Trifluoromethyl)-9H-thioxanthen-9-ylidene]propyl]-1-piperazineethanol dihydrochloride (flupenthixol dihydrochloride), [S-(R*,R*)]-[3-[[1-(3,4-Dichlorophenyl)ethyl]amino]-2-hydroxypropyl](cyclohexylmethyl) phosphinic acid (CGP 54626 hydrochloride), 8,8’-[Carbonylbis[imino-3,1-phenylenecarbonylimino(4-methyl-3,1-phenylene)carbonylimino]]bis-1,3,5-naphthalenetrisulfonic acid hexasodium salt (suramin hexasodium salt), N-(Piperidin-1-yl)-5-(4-iodophenyl)-1-(2,4-dichlorophenyl)-4-methyl-1H-pyrazole-3-carboxamide (AM 251), D-(−)-2-Amino-5-phosphonopentanoic acid (D-AP5), 6-Cyano-7-nitroquinoxaline-2,3-dione disodium (CNQX disodium salt), (S)-(+)-α-Amino-4-carboxy-2-methylbenzeneacetic acid (LY367385), and 2-Methyl-6-(phenylethynyl)pyridine hydrochloride (MPEP hydrochloride), Octahydro-12-(hydroxymethyl)-2-imino-5,9:7,10a-dimethano-10aH-[1,3]dioxocino[6,5-d] pyrimidine-4,7,10,11,12-pentol (Tetrodotoxin: TTX) were purchased from Tocris Bioscience. Picrotoxin from Indofine Chemical Company (Hillsborough, NJ). Fluo-4-AM from Molecular Probes (Eugene, OR). All other drugs were purchased from Sigma.

Corkrum M., Covelo A., Lines J., Bellocchio L., Pisansky M., Loke K., Quintana R., Rothwell P.E., Lujan R., Marsicano G., Martin E.D., Thomas M.J., Kofuji P, & Araque A. (2020). Dopamine-Evoked Synaptic Regulation in the Nucleus Accumbens Requires Astrocyte Activity. Neuron, 105(6), 1036-1047.e5.

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Neurophysiological Recording Reagents

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4-Aminopyridin (4-AP, 100 mM; Merck), D-AP5 (50 mM; Tocris), and tetrodotoxin (TTX, 1 mM; Alomone labs) were dissolved in water. Picrotoxin was dissolved at 50 mM in ethanol. CGP 54626 hydrochloride (10 mM; Tocris), CNQX (20 mM; Tocris), NBQX (20 mM; Tocris), L-655,708 (1 mM; Tocris), and the α5-NAM RO4938581 (10 mM; F. Hoffmann-La Roche) were dissolved in DMSO.

Schulz J.M., Knoflach F., Hernandez M.C, & Bischofberger J. (2018). Dendrite-targeting interneurons control synaptic NMDA-receptor activation via nonlinear α5-GABAA receptors. Nature Communications, 9, 3576.

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Neurochemical Reagents for Research

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Flupenthixol dihydrochloride, CGP54626 hydrochloride, suramin hexasodium salt, AM 251, D-AP5, CNQX disodium salt, LY367385, MPEP hydrochloride, and Tetrodotoxin (TTX) were purchased from Tocris Bioscience. Picrotoxin was purchased from Indofine Chemical Company (Hillsborough, NJ). Fluo-4-AM from Molecular Probes (Eugene, OR). All other drugs were purchased from Sigma.

Nanclares C., Poynter J., Martell-Martinez H.A., Vermilyea S., Araque A., Kofuji P., Lee M.K, & Covelo A. (2023). Dysregulation of astrocytic Ca2+ signaling and gliotransmitter release in mouse models of α-synucleinopathies. Acta Neuropathologica, 145(5), 597-610.

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Baclofen Dendritic Stimulation Protocol

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Baclofen (50 μM) was pressure ejected from a glass pipette (tip diameter: 1 μm) placed 50–100 μm distal to the dendritic patch pipette (approx. 500–800 μm from the soma). The volume directly affected by the drugs pressure ejected from the puffing pipette was estimated to have a radius of approx. 100 μm, as measured by a test application of the fluorescent indicator Alexa 594 into a brain slice. The GABA_BR antagonist CGP 54626 hydrochloride (Tocris) was dissolved in DMSO (10 mM). Nimodipine (Sigma) was dissolved at 20 mM in DMSO on the day of the experiment. In experiments testing GIRK channel contribution, tertiapin (Sigma) was added to the bath ACSF (0.5 μM) and puff pipette (5.0 μM).

Schulz J.M., Kay J.W., Bischofberger J, & Larkum M.E. (2021). GABAB Receptor-Mediated Regulation of Dendro-Somatic Synergy in Layer 5 Pyramidal Neurons. Frontiers in Cellular Neuroscience, 15, 718413.

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Neurotransmitter Receptor Antagonists in Fly

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Picrotoxin (Sigma), CGP54626 hydrochloride (Tocris Bioscience), mecamylamine hydrochloride (Sigma-Aldrich), (+)-MK-801 hydrogen maleate (Sigma-Aldrich), and (+)-butaclamol hydrochloride (Sigma-Aldrich) were used as receptor antagonists of GABA_A, GABA_B, nicotinic acetylcholine, NMDA, and dopamine, respectively. Picrotoxin, mecamylamine hydrochloride, and (+)-MK-801 hydrogen maleate were dissolved in saline to 500 μM, 200 μM, and 200 μM, respectively. Both CGP54626 hydrochloride and (+)-butaclamol hydrochloride were dissolved in dimethyl sulfoxide (DMSO) to 100 mM and further diluted in saline to 100 μM. These drug solutions were stored at -30°C until use. During drug application, 1 mL of the saline applied on the fixed fly was first discarded and then equivalent amounts of drug solutions or saline as control were applied. Final concentrations of Picrotoxin, CGP54626 hydrochloride, mecamylamine hydrochloride, (+)-MK-801 hydrogen maleate, and (+)-butaclamol hydrochloride were 250 μM, 50 μM, 100 μM, 100 μM, and 50 μM, respectively.

Tachibana S.I., Touhara K, & Ejima A. (2015). Modification of Male Courtship Motivation by Olfactory Habituation via the GABAA Receptor in Drosophila melanogaster. PLoS ONE, 10(8), e0135186.

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